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    Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology by Bowen Chu, Yao Shi, Zhimin Li, Hao Tian, Wanyi Li, Yuanzhong Wang

    Published 2015-01-01
    “…The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33°C, and ratio of solvent to raw material of 10.21 : 1 mL/g. …”
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    黑颈鹤源大肠杆菌的快速鉴定及致病特性的初步研究 by 余绍华, 陈武, 莫卓东

    Published 2011-01-01
    “…采用16S rRNA序列分析法鉴定黑颈鹤病原菌,其毒力基因分别用大肠杆菌的irp2、papC、iucD、tsh、iss毒力基因的特异性引物进行PCR检测,并将扩增出来的irp2基因序列与Genbank<sup>TM</sup>相应序列进行同源性分析。…”
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