Replacement of fishmeal with Quinoa Husk (Chenopodium quinoa) for mitigating multiple stresses in Pangasianodon Hypophthalmus by Neeraj Kumar, Paritosh Kumar, Aliza Pradhan, Dilip Kumar Singh, Tarkeshwar Kumar, Jagadish Rane, Kotha Sammi Reddy

“…The aspartate amino transferase (AST) and alanine amino transferase (ALT) as well as malate dehydrogenase (MDH) and lactate dehydrogenase (LDH) in gill and liver were significantly reduced by QH at 25% in fish reread under multiple stresses (NH3+As+T). The nitro blue tetrazolium (NBT), blood glucose, albumin, globulin, total protein, A:G ratio, myeloperoxidase (MPO) and total immunoglobulin (Ig) were noticeably improved by supplementation of QH at 25–30% in fish reared under NH3+As+T. …”
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Semen Parameters Can be Used as a Credible Marker with Doppler Ultrasonography in the Diagnosis of Subclinical Varicocele Cases by Tulay Irez, Bahar Sarikaya, Mine Erguven, Emre Salabas

“…Sperm were dyed with aniline blue and chromomycin A3 (CMA3), acridine orange (AO), propidium iodide (PI), and Rhodamine 123 (Rh123) for the determination of sperm maturation, sperm DNA fragmentation, apoptosis, and sperm mitochondrial membrane potential, respectively. …”
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Highly corrosion-resistant and photocatalytic hybrid coating on AZ31 Mg alloy via plasma electrolytic oxidation with organic-inorganic integration by Talitha Tara Thanaa, Mohammad Aadil, Alireza Askari, Arash Fattah-alhosseini, Mohammad Alkaseem, Mosab Kaseem

“…Additionally, the coating achieved complete (100%) degradation of methylene blue (MB) within 30 min under visible light. Density Functional Theory (DFT) calculations provide deeper insights into the bonding mechanisms and interaction stability between PHEN, 2-IMD, and the PEO layer on the Mg alloy and MB dye. …”
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Local Overexpression of Interleukin-11 in the Central Nervous System Limits Demyelination and Enhances Remyelination by Anurag Maheshwari, Kris Janssens, Jeroen Bogie, Chris Van Den Haute, Tom Struys, Ivo Lambrichts, Veerle Baekelandt, Piet Stinissen, Jerome J. A. Hendriks, Helena Slaets, Niels Hellings

“…When IL-11 expressing LV was therapeutically applied in animals with extensive demyelination, a significant enhancement of remyelination was observed as demonstrated by Luxol Fast Blue staining and electron microscopy imaging. Our results indicate that IL-11 promotes maturation of NG2+ OPCs into myelinating CC1+ OLs and may thus explain the enhanced remyelination. …”
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Immunogenicity of recombinant fragment of orthopoxvirus p35 protein in mice by Ya. A. Khlusevich, A. L. Matveev, E. P. Goncharova, I. K. Baykov, N. V. Tikunova

“…In this work, recombinant protein р35Δ12 produced in E. coli cells XL1-blue and purified by chromatography was used for two-time immunization of mice. …”
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A multifactor coupled rarly-warning model for pipeline landslide hazards based on the dynamic evolutionary process of disaster by Sen WU, Dexu QI, Hu LI, Yu LAN, Jiang LI, Mingzhi SUN

“…This early-warning model was applied in a natural gas pipeline landslide in Guizhou Province, and successfully issued blue, yellow, and red early-warning information, providing sufficient time for emergency response and rescue of the landslide, ensuring the safe operation of the pipeline. …”
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Catalytic degradation of dyes using gold nanoparticles supported over chitosan coated cotton cloth composite by Awais, Fahim Ullah, Adnan Khan, Romana Khan, Sher Bahadar Khan, Khalid A Alzahrani, Nauman Ali, Mazhar Ul-Islam, Muhammad Tariq Saeed Chani, Tahseen Kamal

“…This research work investigates the synthesis of gold nanoparticles (Au NPs) on chitosan-coated cotton cloth (CH-CC) as a versatile catalyst for the reduction of hazardous dyes, specifically methylene blue (MB), rhodamine B (RB), and congo red (CR). …”
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Anticancer activity of mixed Graphene/Carbone nanotubes reinforced Sulfur-Containing polyketoamine nanocomposites by Khalid A. Khan, Gadeer R. Ashour, Mahmoud A. Hussein

“…The nanocomposites also showed pronounced anticancer effects, with PKA/GNS/MWCNTsa (IC50 = 92.89 μg/mL) being the most effective, followed by PKA/GNS/MWCNTsd (IC50 = 104.0 μg/mL), PKA/GNS/MWCNTsc (IC50 = 109.5 μg/mL), PKA/GNS/MWCNTsb (IC50 = 110.1 μg/mL), and pure PKA (IC50 = 149.4 μg/mL). Methylene blue staining confirmed the suppression of cancer cell growth, particularly with PKA/GNS/MWCNTsd, highlighting their potential for clinical applications.…”
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Knowledge, attitudes, and practices of Native Americans in northern California regarding ticks and tick-borne diseases by Patricia Torres, Elizabeth F. Pienaar, Michelle A. Ritchie, Mourad W. Gabriel, Michael J. Yabsley

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Soil organic carbon contents and their major influencing factors in mangrove tidal flats: a comparison between estuarine and non-estuarine areas by Ting Wu, Jia Guo, Gang Li, Yu Jin, Wei Zhao, Guangxuan Lin, Fang-Li Luo, Yaojun Zhu, Yifei Jia, Li Wen

“…Abstract Background Unvegetated tidal flats of mangrove ecosystems in estuarine and non-estuarine areas play a crucial role in the coastal blue carbon sinks and biogeochemical cycle. However, there is still a lack of comprehensive understanding of the differences in soil organic carbon (SOC) contents in mangrove tidal flats between estuarine and non-estuarine areas, as well as their influencing factors. …”
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Integrating Remote Sensing and Soil Features for Enhanced Machine Learning-Based Corn Yield Prediction in the Southern US by Sayantan Sarkar, Javier M. Osorio Leyton, Efrain Noa-Yarasca, Kabindra Adhikari, Chad B. Hajda, Douglas R. Smith

“…Sixty-two different predictors, including soil properties (sand, silt, and clay percentages), slope, spectral bands (red, green, blue, red-edge, NIR), vegetation indices (GNDRE, NDRE, TGI), color-space indices, and wavelengths were derived from the multispectral data collected at the seven (V4, V5, V6, V7, V9, V12, and V14/VT) growth stages of corn. …”
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Ecological risks in downstream lakes exacerbated by the three Gorges reservoir by Xinxin Chen, Yaxin Guo, Junhong Zhang, Xiao Han, Weiya Huang, Yao Yue

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Establishment and validation of HPLC methods for the determination of folic acid and parabens antimicrobial agents on folic acid oral solution by Wenhong Wu, Ying Liang, Renbang Zhao, Yude Shi, Jiahui Hou, Jiumei Peng, Jiadi Pan, Xiaoyi Li, Jingjing Zhou

“…The flow rate used was 1.2 mL/min, the injection volume of folic acid was 20 µL, and the injection volume of bacteriostatic agent was 50 µL. In addition, the blue applicability grade index (BAGI) and analytical greenness (AGREE) metric tools were used to evaluate the greenness and environmental friendliness of the developed methods. …”
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Mechanism of Spica Prunellae in Suppressing Proliferation and Stem Cell Properties of Colorectal Cancer Cells Based on Notch Signaling Pathway by TIAN Haoyuan, XIE Yi, FANG Yi, CHENG Ying, LIN Yan, SU Daifeng, WEI Lihui

“…The number of viable cells was detected by 0.4% Trypan blue staining and cell survival rate was calculated. …”
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A comparative study of mitoxantrone hydrochloride injection and technetium-99 m for tracing axillary sentinel nodes in patients with breast cancer by Dechuang Jiao, Xiuchun Chen, Xianfu Sun, Zhenduo Lu, Hao Dai, Chengzheng Wang, Chongjian Zhang, Lianfang Li, Jianghua Qiao, Zhenzhen Liu

“…The SLN detection rate did not significantly differ between MHI and 99mTc. MHI blue staining was an independent predictor of pathological positivity (OR 3.58, 95% CI: 1.21–10.60, p = 0.02), while the 99mTc signal was not significant in the same model (p = 0.39). …”
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In Vitro Effect of Estradiol and Progesterone on Ovine Amniotic Epithelial Cells by Annunziata Mauro, Hashimita Sanyal, Angelo Canciello, Paolo Berardinelli, Valentina Russo, Nicola Bernabò, Luca Valbonetti, Barbara Barboni

“…Conversely, P4 exposure inhibited oAEC chondrogenic differentiation, which was induced in E2-treated cells as confirmed by the upregulation of chondrogenesis-related genes (SOX9, ACAN, and COL2A1) and by the accumulation of Alcian blue-positive extracellular matrix. Simultaneously, E2-treated cells remained unresponsive to osteogenic inductive stimuli. …”
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Unveiling the UNESCO Biosphere Reserve of the Berlengas Archipelago in Portugal as a Hotspot of Fish Species Using eDNA Metabarcoding and the Collaboration of Fishing Crews by Marco Simões, Cátia Costa, Maria da Luz Calado, Nuno Vasco-Rodrigues, Maria Jorge Campos, Sérgio Miguel Leandro, Agostinho Antunes

“…Our study also highlighted eDNA’s role in monitoring fish species by detecting unexpected species for the region, such as Atlantic salmon (<i>Salmo salar</i>) and Atlantic cod (<i>Gadus morhua</i>), while cautioning against false positives like orange clownfish (<i>Amphiprion percula</i>) and blue tilapia (<i>Oreochromis aureus</i>). Future optimisation of our eDNA sampling methodology could better refine marine ecosystem dynamics around the UNESCO Biosphere Reserve of the Berlengas Archipelago, Portugal.…”
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Eco-Friendly Synthesis of Zirconium Dioxide Nanoparticles from <i>Toddalia asiatica</i>: Applications in Dye Degradation, Antioxidant and Antibacterial Activity by Arumugam Kathirvel, Ramalingam Srinivasan, Sathasivam Harini, Natarajan Ranjith, Govindan Suresh Kumar, Kesavan Lalithambigai, Raji Atchudan, Mohamed A. Habila, Ahmed M. Aljuwayid, Hae Keun Yun

“…The ZrO₂ NPs demonstrated remarkable photocatalytic activity, degrading over 95% of methylene blue dye after 3 h of sunlight exposure. Additionally, the ZrO₂ NPs exhibited strong antibacterial effects, particularly against Gram-negative bacteria such as <i>E</i>. …”
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Benzothiazole (BTH) Induced Resistance of Navel Orange Fruit and Maintained Fruit Quality during Storage by Huaying Du, Ying Sun, Rui Yang, Wei Zhang, Chunpeng Wan, Jinyin Chen, İbrahim Kahramanoğlu, Liqin Zhu

“…Our results suggested that BTH treatment may be a promising treatment for maintaining the quality and inhibiting blue mold of postharvest navel orange in the future.…”
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Using Recombinase-Aid Amplification Combined with <i>Pyrococcus furiosus</i> Argonaute for Rapid Sex Identification in Flamingo (<i>Phoenicopteridae</i>) by Shenluan Tan, Fanwen Zeng, Wanhuan Zhong, Tanzipeng Chen, Xuanjiao Chen, Li Li, Hengxi Wei, Shouquan Zhang

“…The greater flamingo-RAA-<i>Pf</i>Ago system can identify unknown-sex greater flamingos in less than 1 h and can be visualized using a fluorescent detector or blue light. The results showed that optimal RAA-<i>Pf</i>Ago conditions could detect 0.6 ng of genomic DNA and effectively differentiate between males and females. …”
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