Aberrant B cell receptor signaling responses in circulating double-negative 2 B cells from radiographic axial spondyloarthritis patients by Rick Wilbrink, Stefan F.H. Neys, Rudi W. Hendriks, Anneke Spoorenberg, Frans G.M. Kroese, Odilia B.J. Corneth, Gwenny M.P.J. Verstappen

“…Methods: We performed immune gene expression profiling on total peripheral blood B cells from 8 r-axSpA patients and 8 healthy controls (HCs). …”
Get full text

Constitutive expression of Cas9 and rapamycin-inducible Cre recombinase facilitates conditional genome editing in Plasmodium berghei by Samhita Das, Tanaya Unhale, Carine Marinach, Belsy del Carmen Valeriano Alegria, Camille Roux, Hélène Madry, Badreddine Mohand Oumoussa, Rogerio Amino, Shiroh Iwanaga, Sylvie Briquet, Olivier Silvie

“…Conventional reverse genetic tools cannot be used to study essential genes of the asexual blood stages, thereby necessitating the development of conditional strategies. …”
Get full text

Characterization, Quantification, and Molecular Identification of Co-Infection of Canine Parvovirus (CPV-2) Variants in Dogs Affected by Gastroenteritis in Ecuador During 2022–2023... by Anthony Loor-Giler, Silvana Santander-Parra, Sara Castillo-Reyes, Martin Campos, Renán Mena-Pérez, Santiago Prado-Chiriboga, Luis Nuñez

“…Severe signs include vomiting, diarrhea with blood, feeling tired, and not drinking enough water. …”
Get full text

Effect of Remote Home Rehabilitation Mode on Children with Cerebral Palsy and Their Families during Epidemic Isolation by Tingting XU, Yunfei QIAO, Mao HUANG

“…Before and after intervention, Gesell scale was used to evaluate the growth and development of the children with CP; the clinical symptom score of traditional Chinese medicine(TCM)was used to evaluate clinical effect; weight scale was used to measure body mass; color doppler ultrasound was used to detecte cerebral hemodynamics indexes such as the cerebral artery mean arterial blood flow velocity(Vm)and vascular resistance index(RI); Hamilton depression scale(HAMD-17)was used to evaluate the depression of the CP children's family members.Results:Before intervention, there were no significant differences in DQ score, clinical symptom score of TCM, Vm, RI index, body mass and HAMD-17 score between two groups(<italic>P</italic>&gt;0.05).Compare with before intervention, body weight, Vm and HAMD-17 scores of the two groups after intervention were significantly increased, DQ score of the observation group after intervention was significantly increased, the RI was significantly decreased, with statistically significant differences(<italic>P</italic>&lt;0.05).Compared with the control group, DQ score and Vm of the observation group after intervention were significantly higher, while clinical symptom score of TCM, RI and HAMD-17 scores of the observation group after intervention were significantly lower, the difference was statistically significant(<italic>P</italic>&lt;0.05).There was no significant difference in body mass between the two groups after intervention(<italic>P</italic>&gt;0.05).Conclusion:Remote home rehabilitation mode can effectively promote the growth and development of CP children, improve the symptoms of tardiness in standing, walking, speaking, soft and hard hands and feet, and relieve the depression of CP children's family members, which is an effective rehabilitation training mode for CP children during the period of epidemic isolation.…”
Get full text

Construction and evaluation of a prognostic model for cervical cancer based on dynamic hematological and clinical features by Huangrong Ma, Lingyu Ma, Li Yang, Miaoying Cai, Yifu Wang, Yue Li, Chunyu Liang, Zhiyuan Xu

“…The prognostic significance of dynamic changes in blood and inflammatory markers has been confirmed. …”
Get full text

Landscape of the Peripheral Immune Response Induced by Intraoperative Radiotherapy Combined with Surgery in Early Breast Cancer Patients by Danian Dai, Xuerui Li, Hongkai Zhuang, Yun Ling, Lezi Chen, Cheng Long, Jinhui Zhang, Yunjie Wang, Yuehua Li, Hailin Tang, Bo Chen

“…In this study, single‐cell RNA sequencing and single‐cell T cell receptor sequencing are conducted on peripheral blood mononuclear cells (PBMCs) from patient with early‐stage breast cancer before and after IORT. …”
Get full text

Effect of propofol and ciprofol in patients undergoing bronchoscopy: protocol for a double-blind, randomised controlled trial by Fang Chen, Cheng Li, Dongliang Li, Jinxuan Tang, Jiayu Huang

“…The primary outcomes are changes in heart rate and blood pressure before and after induction, laryngeal mask airway (LMA) placement and bronchoscopy placement. …”
Get full text

Expression of lipid metabolism- related genes, body composition and inflammation: Effects of sodium butyrate supplementation in subjects with obesity by Parichehr Amiri, Seyed Ahmad Hosseini, Maryam Saghafi-Asl, Amin Akbari-Naserkiadeh, Seyedeh Asal Jenani Zavieh, Sara Arefhosseini, Samira Asghari, Neda Roshanravan

“…The present triple-blind, randomized clinical trial examined the effects of NaBu supplementation on the expression of lipid metabolism- related genes in peripheral blood mononuclear cells, body composition compartments and serum high-sensitivity C-reactive protein (hs-CRP) in subjects with obesity. …”
Get full text

Study of the neuroprotective properties of metformin in rats with type 2 diabetes mellitus and brain injury induced by intracerebral hemorrhage by V.L. Holubiev, M.H. Oberemok, V.A. Tkachenko, Yu.V. Kharchenko, O.O. Bondarenko, A.E. Lievykh, V.I. Zhyliuk

“…Considering the increase in VE-cadherin expression by the drug, it is possible to predict its positive effect on the function of blood-brain barrier. This study may serve as a reference for the feasibility of studying the clinical efficacy of metformin under these conditions.…”
Get full text

A pilot study examining Toronto-area family physician perspectives on thyroid neoplasm evaluation by Arjun Kundra, David P. Goldstein, Kimberly Wintemute, Sangeet Ghai, Richard W. Tsang, Karuna Gupta, Donatus R. Mutasingwa, Jeff Weissberger, Ella Huszti, Patrick Brown, Huan Jiang, Anna M. Sawka

“…Although appropriate indications for TUS were endorsed by most respondents (e.g. palpable TN, incidental TN on other imaging), inappropriate recommendations were observed in a third of FPs (19/57) who recommended TUS for abnormal thyroid blood tests about half of FPs (30/56) who endorsed biopsy of sub-centimeter nodules. …”
Get full text

Plasma circulating cell–free DNA integrity and relative telomere length as diagnostic biomarkers for Parkinson’s disease and multiple system atrophy: a cross-sectional study by Chao Ying, Chao Han, Yuan Li, Mingkai Zhang, Shuying Xiao, Lifang Zhao, Hui Zhang, Qian Yu, Jing An, Wei Mao, Yanning Cai

“…This study investigated the total concentration, nonapoptotic level, integrity, and cell-free DNA relative telomere length of cell-free DNA in the peripheral blood of 171 participants, comprising 76 normal controls, 62 patients with Parkinson’s disease, and 33 patients with multiple system atrophy. …”
Get full text

Effect of Simple Reaction Speed Training on Executive Function of the Elderly with Mild Cognitive Impairment by Hong ZHANG, Xiaofei WEI, Congyong LIU, Kaili QIAN, Chun LI, Rang LI, Yuan JIANG

“…Moreover, the serum brain-derived neurotrophic factor in the blood before and after intervention was detected by using enzyme linked immunosorbent assay (ELISA), respectively.Results:Compared with before intervention, the scores of conversion, working memory, planning, task monitoring, MI, total standard scores, TMT-B and interference in the observation group after intervention for three months were significantly lower, KST, TJT, ZMT, total standard scores and level of serum BDNF of the observation group after intervention were significantly higher (<italic>P</italic>&lt; 0.05); compared with the control group, the scores of conversion, task initiation, working memory, planning, BRI, MI, total standard scores, TMT-B and interferences of the observation group were significantly lower, and RSCT, KST, TJT, ZMT, total standard scores and level of serum brain-derived neurotrophic factor (BDNF) of the observation group were significantly high (<italic>P</italic>&lt; 0.05).Conclusion:Simple reaction speed training can improve the executive function, brain nerve function, and effectively delay cognitive decline of elderly with MCI.…”
Get full text

Centrosome amplification and aneuploidy driven by the HIV-1-induced Vpr•VprBP•Plk4 complex in CD4+ T cells by Jung-Eun Park, Tae-Sung Kim, Yan Zeng, Melissa Mikolaj, Jong Il Ahn, Muhammad S. Alam, Christina M. Monnie, Victoria Shi, Ming Zhou, Tae-Wook Chun, Frank Maldarelli, Kedar Narayan, Jinwoo Ahn, Jonathan D. Ashwell, Klaus Strebel, Kyung S. Lee

“…We observe that approximately 1–5% of CD4+ T cells from the blood of people living with HIV-1 exhibit over-duplicated centrioles, suggesting that centrosome amplification underlies the development of HIV-1-associated cancers by driving aneuploidy. …”
Get full text

Benefits of physical exercise through multivariate analysis in sedentary adults and elderly: An analysis of physical fitness, health and anthropometrics by Guilherme da Silva Rodrigues, Andressa Crystine da Silva Sobrinho, Gabriel Peinado Costa, Leonardo Santos Lopes da Silva, João Gabriel Ribeiro de Lima, Leonardo da Silva Gonçalves, Larissa Chacon Finzeto, Carlos Roberto Bueno Júnior

“…Results showed that participants aged 71 and above had lower systolic blood pressure, height, body mass, elbow flexion and extension, sitting and standing up from a chair, agility, 6-Minute Walk Test (6MWT), and handgrip strength values compared to younger age groups (p < 0.05). …”
Get full text

C1q/TNF-Related Protein5 (CTRP5) as a Biomarker to Predict Metabolic Syndrome and Each of Its Components by Feiyu Jiang, Min Yang, Xili Zhao, Rui Liu, Gangyi Yang, Dongfang Liu, Hua Liu, Hongting Zheng, Zhiming Zhu, Ling Li

“…Circulating CTRP5 was found to be correlated negatively with BMI, FAT%, FBG, WHR, SBP, HbA1c, TG, 2-hour blood glucose after glucose overload (2-hOGTT), FIns, and HOMA-IR and positively with HDL-C (P<0.05 or P<0.01). …”
Get full text

Jiawei Suanzaoren decoction for the treatment of perimenopausal insomnia: clinical observation and experimental study by Li-Qiong Chen, Yong-Can Zhou, Fang Ning, Ming-Jin Zhu

“…The sleep condition, the levels of blood hormone levels and the expression levels of 5-Hydroxytryptamine (5-HT) and Gamma-Aminobutyric Acid receptor A (GABAA) receptor in the hypothalamus were compared between groups.ResultsIn clinical observation, the sleep condition and the changes of hormone levels between the treatment group and the control group were statistically significant (P &lt; 0.05). …”
Get full text

Unraveling the pathogenesis of Barrett’s esophagus and esophageal adenocarcinoma: the “omics” era by Alberto Barchi, Giuseppe Dell’Anna, Giuseppe Dell’Anna, Luca Massimino, Luca Massimino, Francesco Vito Mandarino, Edoardo Vespa, Edi Viale, Sandro Passaretti, Vito Annese, Vito Annese, Alberto Malesci, Alberto Malesci, Silvio Danese, Silvio Danese, Federica Ungaro

“…Increasing interest has been gathered around small circulating genetic traces (circulating-free DNA and micro-RNAs) with a potential role as blood biomarkers. Epigenetic alterations (such as hyper or hypo-methylation) play a meaningful role in esophageal carcinogenesis as well as the study of the tumor micro-environment, which has led to the development of novel immunological therapeutic options. …”
Get full text

Clinical characteristics of EGFR-ctDNA shedders in EGFR-mutant NSCLC patients by Martina Ruglioni, Iacopo Petrini, Stefania Crucitta, Andrea Sbrana, Giovanna Irene Luculli, Leila Sadeghi Gol, Carola Forte, Antonio Chella, Christian Rolfo, Romano Danesi, Marzia Del Re

“…This study aims to understand the determinants for ctDNA shedding based on clinical characteristics of lung cancer patients, for a better interpretation of false negative results to be considered when ordering ctDNA analysis for clinical practice. Methods: Blood samples were collected from patients with stage IV EGFR-mutated (mEGFR) NSCLC before treatment and monitored until disease progression. …”
Get full text

Alopecia Management Potential of Rosemary-Based Nanoemulgel Loaded with Metformin: Approach Combining Active Essential Oil and Repurposed Drug by Abla KK, Alamoudi MK, Soliman GA, Abdel-Kader MS, Aldawsari MF, Mehanna MM

“…Hence, the current study aims to fabricate a safe and effective nanoemulsion to improve metformin efficacy in targeting AGA.Methods: Rosemary oil was selected as the oily phase due to its ability to increase blood flow and hair growth. Rosemary-based nanoemulsions were statistically optimized by Box-Behnken experimental design, loaded with metformin, and incorporated into a hydrogel to form a nanoemulgel. …”
Get full text

Nonlinear association between glycated hemoglobin levels and mortality in elderly patients with non-diabetic chronic kidney disease: a national health and nutrition examination sur... by Lihua Huang, Liuliu He, Qingfeng Zeng, Jinjing Huang, Xiaoyan Luo, Qiuming Zhong

“…This study aimed to investigate the correlation between HbA1c levels and all-cause and cardiovascular disease (CVD) mortality in elderly individuals with non-diabetic CKD.MethodsData from the NHANES (1999-2018) were analyzed to measure HbA1c levels in whole blood using high-performance liquid chromatography (HPLC). …”
Get full text