Extracellular Vesicles of Mesenchymal Stem Cells Are More Effectively Accessed through Polyethylene Glycol-Based Precipitation than by Ultracentrifugation by Lei Jia, Bo Li, Cong Fang, Xiaoyan Liang, Yingjun Xie, Xiaofang Sun, Wen Wang, Lei Zheng, Ding Wang

“…We harvested EVs from amniotic fluid MSCs (AF-MSCs) by UC- or PEG-based precipitation methods and conducted a comparison study of those EVs derived by the two methods: output, RNA, and protein expression of EVs and EV biological reaction in a THP-1-cell model of LPS induction, which was considered an infection model. …”
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Choosing between GLP-1 Receptor Agonists and DPP-4 Inhibitors: A Pharmacological Perspective by Dominique Xavier Brown, Marc Evans

“…GLP-1 receptor agonists such as liraglutide and exenatide exert an intrinsic biological effect on GLP-1 receptors directly stimulating the release of insulin from pancreatic beta cells. …”
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Indoor air quality at the Arab governmental girls’ schools [version 2; peer review: 2 approved, 1 approved with reservations] by Abdulmalik Salman Alsaif, Mohammed Tawfiq Aljassim, Sana Abdullah Alsulaiman, Mahmoud Fathy Elsharkawy

“…The physical school environment includes the physical structures; presence of chemicals and biological agents; and the surrounding environment, including air, water, and materials. …”
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Whole-Genome Expression Analysis and Signal Pathway Screening of Synovium-Derived Mesenchymal Stromal Cells in Rheumatoid Arthritis by Jingyi Hou, Yi Ouyang, Haiquan Deng, Zhong Chen, Bin Song, Zhongyu Xie, Peng Wang, Jinteng Li, Weiping Li, Rui Yang

“…A Gene Ontology analysis showed significantly enriched terms of differentially expressed genes in the biological process, cellular component, and molecular function domains. …”
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Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit Fungi by Maxwell Mewa Ngongang, Heinrich Wilbur Du Plessis, Chidi Silas Boredi, Ucrecia Faith Hutchinson, Karabo Seteno Obed Ntwampe, Vincent Ifeanyi Okudoh, Neil Paul Jolly

“…Therefore, the preventive use of P. kluyveri against post-harvest fruit-fungal infections proved to be an effective method for biological control of grapes, apples, and pears against fungal spoilage organisms Botrytis cinerea and Monilinia laxa .…”
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Enhancing human ACE2 expression in mouse models to improve COVID‐19 research by Sun Jiaoyang, Cheng Shaofei, Hong Guangliang, Quan Xiongzhi, Lin Haofeng, Mao Rui, Johannes Grillari, Shi Zheng‐Li, Chen Jiekai, Liu Meiqin, Wu Haoyu, Wu Guangming

“…Mice are one of the most common biological models for laboratory use. However, wild‐type mice are not susceptible to COVID‐19 infection due to the low affinity of mouse ACE2, the entry protein for SARS‐CoV‐2. …”
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Psychologic Resilience and Related Factors in the Euthymic Bipolar Patients by Ersin Uygun, Aylin Koseoglu, Suat Kucukgoncu, Sahap N. Erkoc

“…Bipolar I disorder is characterized by full blown manic episodes and is not only affected by biological factors but also by psychological and cultural factors. …”
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Integrated Bioinformatics-Based Identification of Potential Diagnostic Biomarkers Associated with Diabetic Foot Ulcer Development by Long Qian, Zhipeng Xia, Ming Zhang, Qiong Han, Die Hu, Sha Qi, Danmou Xing, Yan Chen, Xin Zhao

“…GO analyses revealed these DEGs to be significantly enriched in biological processes including sarcomere organization, muscle filament sliding, and the regulation of cardiac conduction, molecular functions including structural constituent of muscle, protein binding, and calcium ion binding, and cellular components including Z disc, myosin filament, and M band. …”
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T Cell Histiocyte Rich Large B Cell Lymphoma Presenting as Hemophagocytic Lymphohistiocytosis: An Uncommon Presentation of a Rare Disease by Uroosa Ibrahim, Gwenalyn Garcia, Amina Saqib, Shafinaz Hussein, Qun Dai

“…An excisional biopsy of the right inguinal lymph node was consistent with a diagnosis of THRLBCL and the patient subsequently received six cycles of chemotherapy with R-CHOP (Rituximab, Cyclophosphamide, Doxorubicin, Vincristine, and Prednisone) after which a PET-CT scan showed no evidence of biologically active disease and ferritin was down to 822 ng/mL. …”
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A Machine Learning-Based Classification of Immunogenic Cell Death Regulators and Characterisation of Immune Microenvironment in Acute Ischemic Stroke by Mengying Wang, Guolian Wei, Shaorui Gu, Zhengyuan Huo, Xue Han

“…Immunogenic cell death (ICD) regulators exert a crucial part in quite a few in numerous biological processes. This study aimed to determine the function and diagnostic value of ICD regulators in acute ischemic stroke (AIS). 31 significant ICD regulators were identified from the gene expression omnibus (GEO) database in this work (the combination of the GSE16561 dataset and the GSE37587 dataset in the comparison of non-AIS and AIS patients). …”
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COEX-Seq: Convert a Variety of Measurements of Gene Expression in RNA-Seq by Sang Cheol Kim, Donghyeon Yu, Seong Beom Cho

“…Next generation sequencing (NGS), a high-throughput DNA sequencing technology, is widely used for molecular biological studies. In NGS, RNA-sequencing (RNA-Seq), which is a short-read massively parallel sequencing, is a major quantitative transcriptome tool for different transcriptome studies. …”
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Romidepsin Promotes Osteogenic and Adipocytic Differentiation of Human Mesenchymal Stem Cells through Inhibition of Histondeacetylase Activity by Dalia Ali, Elna P. Chalisserry, Muthurangan Manikandan, Rimi Hamam, Musaad Alfayez, Moustapha Kassem, Abdullah Aldahmash, Nehad M. Alajez

“…Pharmacological inhibition of FAK signaling during adipogenesis or inhibition of FAK or TGFβ signaling during osteogenesis diminished the biological effects of romidepsin on BMSCs. The results of chromatin immunoprecipitation combined with quantitative polymerase chain reaction indicated a significant increase in H3K9Ac epigenetic markers in the promoter regions of peroxisome proliferator-activated receptor gamma (PPARγ) and KLF15 (related to adipogenesis) or SP7 (Osterix) and alkaline phosphatase (ALP) (related to osteogenesis) in romidepsin-treated BMSCs. …”
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Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics by Jun-ming Zhang, Huan Han, Bing Fu, Yi-chao Li, Kang Li, Jing-wei Liu, Er-meng Yu, Li-ping Liu

“…This study provides a robust protocol integrating in vivo, in vitro, and in silico approaches to elucidate geosmin's target proteins in grass carp gill tissue, advancing our understanding of pollutant-biological interactions and enhancing environmental risk assessment accuracy.…”
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ESSENTIAL OILS PHARMACOPEIALIDENTIFICATION TESTS AND USES by Mobeen Islam

“… Essential oils are recognized for their medicinal importance since ancient time and they continue to be of paramount significance, proved by the number of studies conducted in biological sciences in recent years. This review deals with the chromatographic techniques for the identification of essential oils, their components, and medicinal uses. …”
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Influence of Solvent on the Component Composition and Antioxidant Properties of Apricot Cake (Prunus armeniaca L.) Extracts by A. S. Shakun, V. I. Vorobyova, O. E. Chygyrynets, M. I. Skiba

“…They have complicated structure and contain a complex of biologically active compounds. This is why research of the above is particularly relevant in current competitive market. …”
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A Promotion Role of MIR31 in the Process of Vocal Fold Wound Healing by Haizhou Wang, Wen Xu

“…Overall, our findings suggest that MIR31 plays a critical role in VFWH, particularly in collagen synthesis and other biological processes, which warrant further investigation.…”
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Analytical Method Development for Sodium Valproate through Chemical Derivatization by Murad Abualhasan, Nihaya Wasif Odeh, Ghadeer Naser Younis, Oyoun Fadel Zeidan

“…The introduction of benzoyl and phenyl groups to the molecule is a useful derivatisation, which enables the creation of detectable chromophores for HPLC analysis for pharmaceutical dosages as well as biological systems. Methodology. Sodium valproate was derivatised by the addition of a chromophore to its structure by introducing a methyl benzoyl or a phenyl group. …”
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Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves by Onoja Samuel O., Nnadi Charles O., Udem Samuel C., Anaga Aruh O.

“…Heliangolide is a naturally occurring sesquiterpene lactone and its derivatives are biologically active compounds present in most medicinal plants. …”
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Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating Hemostasis by Hui-Fang Lin, Yue-Yue Wang, Feng-Zhen Liu, Zi-Wei Yang, Hao Cui, Si-Yu Hu, Feng-He Li, Pei Pan

“…Most of the existing hydrogel dressings have inadequacies in mechanical performance, biological activities, compatibility, or versatility, which results in the development of rapid, green, and cost-effective approaches for hydrogels in biochemical and biomedical applications becoming a top-priority task. …”
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Exploring TNFR1: from discovery to targeted therapy development by Yingying Li, Ruiwei Ye, Haorui Dai, Jiayi Lin, Yue Cheng, Yonghong Zhou, Yiming Lu

“…In conclusion, this study contributes novel insights into the biological functions of TNFR1 and presents potential therapeutic strategies for clinical application, thereby having substantial scientific and clinical significance.…”
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