Cytokine and Chemokine Profile in Patients with Multiple Myeloma Treated with Bortezomib by Paweł Robak, Edyta Węgłowska, Izabela Dróżdż, Damian Mikulski, Dariusz Jarych, Magdalena Ferlińska, Ewa Wawrzyniak, Małgorzata Misiewicz, Piotr Smolewski, Wojciech Fendler, Janusz Szemraj, Tadeusz Robak

“…Serum cytokine levels were assayed with Bio-Rad Bio-Plex Pro Human Cytokine 27-Plex Assay on the MAGPIX Multiplex Reader and the Bio-Plex® 200 System (Bio-Rad). …”
Get full text

Rapid method for identification of transgenic fish zygosity by . Alimuddin, G. Yoshizaki, O. Carman

“…The qr-PCR was performed using iQ SYBR Green Supermix in the iCycler iQ Real-time PCR Detection System (Bio-Rad Laboratories, USA).  Data were analyzed using the comparative cycle threshold method.  …”
Get full text

Composition of High Molecular Weight Glutenin Subunits in Polish Common Wheat Cultivars (Triticum aestivum L.) by Ewa Filip

“…The results were then analyzed using the following computer programs: Finch TV, BLAST, MEGA 4, Molecular Imager® Gel Doc™ XR, and Quantity One software (Bio-Rad). Three alleles (a, b, c) have been identified in the Glu-A1 locus, 6 alleles (a, b, c, d, e, k) in the Glu-B1 locus, and 2 alleles (a, d) in Glu-D1 using the SDS-PAGE method. …”
Get full text

Diagnostic Performance of Three rK39 Rapid Diagnostic Tests and Two Direct Agglutination Tests for the Diagnosis of Visceral Leishmaniasis in Southern Iran by Zahra Rezaei, Bahman Pourabbas, Vera Kühne, Parham Pourabbas, Philippe Büscher

“…The index tests were two direct agglutination tests, DAT-ITM (Institute of Tropical Medicine, Antwerp, Belgium) and DAT-KIT (Royal Tropical Institute, Amsterdam, The Netherlands), and three rapid diagnostic tests (RDTs), Kalazar Detect (InBios International Inc., USA), IT Leish (Bio-Rad, catalog 710124), and Leishmania test (Cypress Diagnostic Company, Belgium). …”
Get full text

SURFACE PLASMON RESONANCE STUDY OF THE INTERACTION BETWEEN THE HUMAN TATA-BOX BINDING PROTEIN AND THE TATA ELEMENT OF THE NOS2A GENE PROMOTER by I. A. Drachkova, S. V. Shekhovtsov, S. E. Peltek, P. M. Ponomarenko, T. V. Arshinova, M. P. Ponomarenko, T. I. Merkulova, L. K. Savinkova, N. A. Kolchanov

“…Thus, we have studied the influence ofSNP (–21 t > c), adjoining to the TATA box ofthe NOS2A promoter, on the interaction by surface plasmon resonance (SPR) on biosensor ProteOn XPR36 (BioRad). The sensograms obtained in the experiment indicate that the constant for the interaction with TATA bearing SNP –21 t > c differ little from the constant with the wild-type box and the dissociation constants differ approximately twofold. …”
Get full text

Comparison of Biomarkers in Blood and Saliva in Healthy Adults by Sarah Williamson, Cindy Munro, Rita Pickler, Mary Jo Grap, R. K. Elswick

“…Cytokines were assayed using Bio-Rad multiplex suspension array technology. Descriptive statistics and pairwise correlations were used for data analysis. …”
Get full text

Colonic growth factors (G-CSF, GM-CSF) and chemokines (MCP-1, MIP-1β) in severe ulcerative colitis by Yevgeny A. Konovich, K. Ye. Shirokikh, M. V. Shapina, Igor L. Khalif

“…Growth factor and chemokine levels in supernatant fluid after centrifugal separation of homogenate of 10 mg biopsy specimen were investigated by the protein analyzer «Bio-Plex» («Bio-Rad», USA). Biopsy specimens of uninvolved colonic mucosa resected in 10 cancer patients were used as a control. …”
Get full text

Identification of Biomarkers of Arrhythmogenic Cardiomyopathy (ACM) by Plasma Proteomics by Sinda Zarrouk, Houda Ben-Miled, Nadia Rahali, Josef Finsterer, Fatma Ouarda

“…Differential protein expressions were assessed using PDQuest software, Bio-Rad US version 8.0.1. <i>Results:</i> The analysis revealed several proteins with altered expressions between ACM patients and controls, including plakophilin-2, junctional plakoglobin, desmoplakin, desmin, transmembrane protein 43, and lamin A/C. …”
Get full text

The Role of Phosphatidylethanolamine-N-methyltransferase (PEMT) Gene rs12449964 Polymorphism in the Development of Acute Pancreatitis and its Complications by T. A. Samgina, Yu. E. Azarova, Yu. V. Kanishchev, V. A. Lazarenko, P. M. Nazarenko, A. V. Polonikov

“…Genotyping of rs12449964 polymorphism was performed using real-time PCR by allelic discrimination using a CFX96 Bio-Rad Laboratories amplifier (USA) with TaqMan probes and commercial TaqMan SNP Genotyping Assays reagents purchased from Applied Biosystems (USA).Results. …”
Get full text

Effectiveness of ISSR markers for detecting genomic variability in <I>Panicum miliaceum</I> L. accessions by D. Kh. Arkhestova, A. A. Yakhutlova, A. D. Khaudov, L. Kh. Sokurova, T. V. Kulemina

“…The DNA of each accession was isolated by the CTAB method, and ISSR analysis was performed in a ­BioRad T100 Thermal Cycler. Statistical processing was made in the DARwin program (version 6.0.21).Results and conclusion. …”
Get full text

Colonic cytokines for severe ulcerative colitis by Ye. A. Konovich, K. Ye. Shirokikh, I. L. Khalif, M. V. Shapina

“…Cytokine concentration was evaluated in supernatant fluid after centrifugal separation of 10 mg biopsy specimen homogenate by the protein analyzer «Bio-Plex» («Bio-Rad», USA). Colonic mucosa biopsy specimens of unaffected parts of the large intestine resected in 10 patients with colorectal cancer were used as controls. …”
Get full text

Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease by Justyna Lorenc-Góra, Dariusz Waniczek, Zenon P. Czuba, Mariusz Kryj, Zbigniew Lorenc, Małgorzata Muc-Wierzgoń

“…We performed the analysis of cytokine concentrations in blood serum using the Bio-Plex Multiplex system (Bio-Rad), and their correlations with radiological parameters were assessed by magnetic resonance enterography (MRE), and fecal calprotectin levels were measured quantitatively by ELISA and clinical evaluation according to the Crohn’s Disease Activity Index (CDAI). …”
Get full text

Serum cytokines in ulcerative colitis of various clinical activity by Ye. A. Konovich, I. L. Halif, M. V. Shapina, K. E. Shirokikh

“…Investigation was carried out by «Bio-Plex» protein analyzer («Bio-Rad», USA). Moderately severe and severe forms of UC were diagnosed in 15 and 18 patients respectively, mild — in 4, resistance and sensitivity for conservative treatment were detected in 15 and 22 patients. …”
Get full text

Surfaces environmental monitoring of SARS-CoV-2: Loop mediated isothermal amplification (LAMP) and droplet digital PCR (ddPCR) in comparison with standard Reverse-Transcription qua... by Simona Spiteri, Irene Salamon, Luna Girolamini, Maria Rosaria Pascale, Federica Marino, Carlo Derelitto, Laura Caligaris, Simone Paghera, Manuela Ferracin, Sandra Cristino

“…Three molecular techniques were compared: Reverse Transcription Loop mediated isothermal AMPlification (RT-LAMP, Enbiotech), Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) (RT-qPCR, Seegene) and droplet digital PCR (ddPCR, Bio-Rad). For ddPCR, two RNA extraction methods were compared: TRIzol LS (Invitrogen) versus QIAmp Viral Mini kit (QIAGEN), showing how the latter is more suitable for surfaces. …”
Get full text