State of the literature discussing smoke-free policies globally: A narrative review by Jacqueline A. Teed, Meagan O. Robichaud, Michelle Duren, Hebe N. Gouda, Ryan David Kennedy

“…We classified articles based on the location of the policy discussed (WHO region, World Bank income classification) and the environment that was being made smoke-free. …”
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人血管内皮生长因子165慢病毒载体的构建及其抗放射所致细胞凋亡的作用

“…【目的】 构建人血管内皮生长因子165(hVEGF165)慢病毒表达载体pCDH-CMV-MCS-EF1-copGFPhVEGF165,并转染至HT22 细胞,并进一步观察其对放射线损伤所致的细胞凋亡的保护作用【方法】 PCR扩增hVEGF165基因,克隆到pCDH-CMV-MCS-EF1-copGFP载体;通过酶切电泳,菌落PCR初步筛选和测序鉴定构建的pCDH-CMV-MCS-EF-GFP1-hVEGF165重组载体;采用磷酸钙共转染法转染293T细胞包装制备慢病毒液,并感染HT22细胞,采用实时RT-PCR及Western Blot检测hVEGF165基因在HT22细胞中的表达情况进一步对单纯HT22细胞空质粒转染组以及hVEGF165转染组HT22细胞进行0510 Gy的X线照射,流式细胞仪检测细胞凋亡情况【结果】 hVEGF165基因片段重组到pCDH-CMV-MCS-EF1-copGFP载体,酶切后电泳结果显示均能得到与理论大小相符的片段,测序结果与GenBank序列完全一致,转染HT22后hVEGF mRNA及蛋白表达水平明显增高而且同各对照组相比,hVEGF165可以降低细胞放疗后的凋亡率【结论】 pCDH-CMV-MCS-EF1-copGFP-hVEGF165 慢病毒表达载体构建成功,其转染HT22细胞后可获得高水平hVEGF165 mRNA和蛋白的表达,hVEGF165具有抗放射所致细胞凋亡的作用…”
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A scoping review on the impact of women’s global leadership: evidence to inform health leadership by Anna Kalbarczyk, Rosemary Morgan, Kelly E Perry, Katherine Banchoff, Charlotte Pram Nielsen, Anju Malhotra

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Prevalence of and factors associated with tobacco smoking in the Gambia: a national cross-sectional study by Abu Naser Zafar Ullah, Md Shariful Islam, Md Golam Rabbani, Haifaa AlWajeah, Md Ferdous, Nusrat Sharmin Mahfuza, Daniel Konka, Eva Silenga

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Rational design of novel phenol ether derivatives as non-covalent proteasome inhibitors through 3D-QSAR, molecular docking and ADMET prediction by Miao Yuan, Hanwen Ji, Fengxin Sun, Qiang Chen, Ping Cheng

“…While the binding models between proteasome [protein data bank (PDB) code: 3MG6] and three representative compounds (15, 20, and D24) were also analyzed by using the molecular docking method. …”
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Validation of In-field Calibration for Low-Cost Sensors Measuring Ambient Particulate Matter in Kolkata, India by Siddharth Nobell, Arnab Majumdar, Shovon Mukherjee, Sukumar Chakraborty, Sanjoy Chatterjee, Soumitra Bose, Anindita Dutta, Sandhya Sethuraman, Daniel M. Westervelt, Shairik Sengupta, Rakhi Basu, V. Faye McNeill

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Effect of NAM-1 genes on the protein content in grain and productivity indices in common wheat lines with foreign genetic material introgressions in the conditions of Belarus by O. A. Orlovskaya, S. I. Vakula, K. K. Yatsevich, L. V. Khotyleva, A. V. Kilchevsky

“…Full-length NAM-A1 nucleotide sequences of T. dicoccoides k-5199, T. dicoccum k-45926, T. kiharae, and T. spelta k-1731 accessions were established and registered with the international molecular database GenBank. Six combinations of NAM-A1/B1 alleles were identified in the accessions studied and their frequency of occurrence varied from 40 to 3 %. …”
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Structural Characterization of B-DNA d(CGTGAATTCACG)₂ in Complex with the Specific Minor Groove Binding Fluorescent Marker Hoechst 33342 by Hristina Sbirkova-Dimitrova, Rusi Rusew, Hristo Gerginov, Annie Heroux, Boris L. Shivachev

“…The coordinates and structure factors are deposited in the RCSB Protein Data Bank (PDB) under entry 9FT8. The structure is nearly isomorphous with that of previously reported crystal structures of the oligonucleotide d(CGTGAATTCACG)₂ alone (PDB ID: 5JU4) and with that in complexes with DAPI (5T4W). …”
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Trauma Volume and Performance of a regional Trauma Center in Korea: Initial 5-year analysis by Byungchul Yu, Giljae Lee, Min A Lee, Kangkook Choi, Sungyoul Hyun, Yangbin Jeon, Yong-Cheol Yoon, Jungnam Lee

“…Methods We analyzed prospectively collected data from the Korean Trauma Data Bank for a single regional trauma center between January 2014 and December 2018. …”
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ENERGY AND ECONOMIC EFFICIENCY OF BIOETHANOL PRODUCTION DEPENDING ON THE QUALITY OF CORN GRAIN by Vitalii Palamarchuk, Roman Lohosha, Vadim Krychkovskyi

“…The results of studies of the influence of foliar fertilisation with a bacterial preparation based on beneficial symbiotic and associative microorganisms Biomag, microfertilisers "ROSTOK" corn, Ecolist Mono Zinc, carried out in the phase of 5-7 and 10-12 leaves of corn, on the level of pre-harvest grain moisture, the number of rows of grains are presented, number of grains in a row, weight of 1000 grains, starch content in grain, productivity and bioethanol yield in hybrids of early maturing group Kharkiv 195 MV (FAO 190) and DKS 2971 (FAO 200), medium early group DKS 3795 (FAO 250) and DKS 3871 (FAO 2480) and medium maturing group DK 315 (FAO 310) and DK 440 (FAO 350) in agro-ecological conditions of the Forest-Steppe of Right-Bank Ukraine. The research is grounded in an evaluation of the efficacy of optimising the supply of plant nutrients through foliar fertilisation in the formation of grain yield and quality. …”
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Randomised trials in maternal and perinatal health in low and middle-income countries from 2010 to 2019: a systematic scoping review by Joshua P Vogel, Jack Williams, Tari Turner, Steve McDonald, Alexander John Eggleston, Annabel Richards, Elise Farrington, Wai Chung Tse, Ayeshini Sella Hewage

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The impact of gestational age limits on abortion-related outcomes: a synthesis of legal and health evidence by Fiona de Londras, Amanda Cleeve, Maria I. Rodriguez, Alana Farrell, Magdalena Furgalska, Antonella F. Lavelanet

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Molecular Detection of Bartonella spp. in China and St. Kitts by Ke Huang, Patrick John Kelly, Jilei Zhang, Yi Yang, Weiguo Liu, Anwar Kalalah, Chengming Wang

“…By comparing highly conserved 16S rRNA sequences of the better-recognized Bartonella spp. on GenBank, we selected primers and probes for a genus-specific pan-Bartonella FRET-qPCR. …”
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Analysis of health expenditures in China from 2000 to 2019 compared with the world and upper-middle-income countries by Xindian Zeng, Xindian Zeng, Lijie Chen, Lu Chen

“…The goal is to provide a theoretical basis for the Chinese government to adjust and formulate health-related policies.MethodsAggregate time-series data were collected from the World Health Organization (WHO) and the World Bank (WB) Open Data sources from 2000 to 2019. These data were compared and some of it analyzed using cluster analysis methods.ResultsThe financing structure level of THE in China is currently above average among upper-middle-income countries. …”
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Assessment of Physicochemical Characteristics of Produced Water from Terminals of Some Oil Industry Facilities in Nigeria by CC Obunwo, C Chukwudi

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Species classification of Tabanus (Diptera: Tabanidae) in Western Thailand: Integrating DNA barcoding and modern morphometrics by Tanasak Changbunjong, Thekhawet Weluwanarak, Sedthapong Laojun, Tanawat Chaiphongpachara

“…To enhance the reliability of species identification, DNA barcoding was employed to analyze 30 cytochrome c oxidase subunit 1 (cox1) gene sequences from 15 horse fly species, which were then compared with sequences in the GenBank and BOLD databases. Most cox1 sequences aligned with existing data, with similarity percentages ranging from 96% to 100%. …”
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Complex bacterial diversity of Guaymas Basin hydrothermal sediments revealed by synthetic long-read sequencing (LoopSeq) by John E. Hinkle, John E. Hinkle, Jeffrey P. Chanton, Molly A. Moynihan, Molly A. Moynihan, S. Emil Ruff, S. Emil Ruff, Andreas Teske

“…In general, LoopSeq-derived 16S rRNA gene sequences aligned consistently with reference sequences in GenBank; however, chimeras were prevalent in sequences as affiliated with the thermophilic Candidatus Desulfofervidus and Thermodesulfobacterium, and in smaller numbers within the sulfur-oxidizing family Beggiatoaceae. …”
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Gender differences in the price elasticity of demand for waterpipe and cigarette smoking in Lebanon, Jordan and Palestine: a volumetric choice experiment by Ramzi G Salloum, Mohammed Jawad, Aya Mostafa, Yousef Khader, Rima Nakkash, Niveen ME Abu-Rmeileh, Ali Chalak, Ruba Abla, Sameera Awawda

“…We also explored the levels of substitution and complementarity in tobacco products among men and women.Setting The study examines tobacco elasticities in three Arab countries: Lebanon, Jordan and the West Bank of Palestine.Participants We used data from nationally representative surveys of adults aged ≥18 years in Lebanon (n=1680), Jordan (n=1925) and Palestine (n=1679). …”
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Catfishes from the North-Western Part of Lake Tanganyika: Contribution to a Reference Library of DNA Barcodes by Archimède Mushagalusa Mulega, Arthur F. Boom, Imane Rahmouni, Hocein Bazairi, Abdelaziz Benhoussa, Maarten P. M. Vanhove, Maarten Van Steenberge

“…We identified 11 species using morphology, compared DNA sequences with those available on GenBank, and employed Automatic Barcode Gap Discovery (ABGD) and phylogenetic approaches. …”
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11 Key Strategies in Using AI Chat GPT to Develop HOTS-Based Entrepreneurship Questionnaires by Almaida Vebibina, Rusijono Rusijono, Andi Mariono, Ahsan Muafa, Dydik Kurniawan, Ragil Sugeng Dewantoro, Dian Noer Asyari, Sussi Widiastuti, Jarot Suseno

“…This finding can be utilized by teachers as the latest generative model to increase the bank of diverse multiple-choice questions without replacing human expertise. …”
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