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2781
In silico analysis of mitochondrial DNA genes: implication for conservation of Tor putitora (Hamilton, 1822)
Published 2025-01-01“…The present study aimed to examine the haplotypic diversity, genetic variation and population structure of T. putitora isolates using COI and Cyt b gene sequences submitted in GenBank. Bioinformatic analysis was carried out using 106 COI and 183 Cyt b gene sequences as well as 2 reference genome sequences. …”
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A deep learning method for differentiating safflower germplasm using optimal leaf structure features
Published 2025-03-01“…The OLSF, calculated as the average response of a multidirectional Gabor filter bank and optimized with the structural similarity index measure (SSIM), enhances complex leaf features, such as veins, texture, and frequency variations, to enhance the classification performance of deep learning models. …”
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2786
Risk factors affecting COVID-19 case fatality rate: A quantitative analysis of top 50 affected countries
Published 2020-11-01“…Datasets for indicators that are prevailing or postulated factors of COVID-19 mortality were extracted from the World Bank database. Out of 185 affected countries, the top 50 countries were selected for analysis in this study. …”
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2787
Molecular Identification of Fusarium proliferatum from toenails: A case report
Published 2019-08-01“…For final identification sequencing of DNA has been carried out by [Macrogen Korea] using forward primer ITS1, The obtained consensus of query sequence was compared with the ITS DNA database on the BLAST homepage, aligned and diagnosed strain deposited to the GenBank and we received an accession number (MK112619). …”
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2788
Knowledge and awareness of Palestinian health care professionals towards probiotics, prebiotics and synbiotics: a cross-sectional study from Palestine
Published 2025-02-01“…A cross-sectional study was conducted across the West Bank using a structured questionnaire, and descriptive analysis was performed using SPSS. …”
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A novel approach for detecting Salmonella enterica strains frequently attributed to human illness—development and validation of the highly pathogenic Salmonella (HPS) multiplex PCR...
Published 2025-01-01“…The utility of the resulting HPS assay for identifying SoC was examined in silico, using BLAST to determine the distribution of gene targets among closed Salmonella genome sequences in GenBank (n = 2,192 representing 148 serotypes) and by assaying 1,303 Salmonella (69 serotypes), isolated from FSIS regulatory samples.Results and discussionComparison of serotypes identified by the assay as HPS, with those identified as SoC, produced an Area Under the Curve (AUC) of 92.2% with a specificity of 96% and a positive predictive value of 97.4%, indicating the HPS assay has strong ability to identify SoC. …”
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2790
Investigation of the validity, reliability and psychometric properties of the Turkish version of the Ottawa sitting scale in patients with Parkinson’s disease
Published 2025-01-01“…The study included 56 patients diagnosed with PD based on the UK Brain Bank Criteria. Construct validity of the OSS-TR was established through the evaluation of structural and convergent validity. …”
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2791
Molecular Detection and Phylogenetic Analysis of the alkB Gene in Klebsiella oxytoca Strains Isolated from the Gut of Tenebrio molitor
Published 2024-01-01“…To detect the presence of the alkB gene in the isolated bacterial strains, a set of primers used in the amplification of this gene was manually designed from the conserved regions of the alkB nucleotide sequences of eleven bacterial species from GenBank. TCOFFE online tool was used to align the alkB sequences of the bacteria, while Jalview and ConSurf were used to view the alignment. …”
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2792
Seeing the Sound: Multilingual Lip Sync for Real-Time Face-to-Face Translation
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Numerical Simulation of Soil Displacement and Settlement in Deep Foundation Pit Excavations Near Water
Published 2021-01-01“…This study uses a numerical simulation method to study the stability of the deep foundation pit near water based on the Biot three-dimensional seepage-stress coupling model, with the open-cut section on the south bank of the Jinan Yellow River Tunnel Project as the engineering field test. …”
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人类Foxm1b基因的原核表达、抗体制备及其对肝癌细胞中Foxm1b蛋白的识别
Published 2007-01-01“…【目的】构建人类Foxm1b基因的原核表达载体,表达并纯化蛋白,制备多克隆抗体,并用此抗体检测肝癌细胞中Foxm1b基因的表达,为进一步研究Foxm1b基因的功能奠定基础。【方法】从GenBank中下载人类Foxm1b基因全长cDNA序列并用Pegene软件分析其可能的抗原表位,设计引物.应用RT—PCR获得含抗原表位的Foxm1b基因片段,重组人原核表达载体pET-28a,在大肠杆菌BL-21(DE31中诱导表达,应用亲和层析法获得纯度较高的原核表达蛋白并免疫大鼠制备多克隆抗体,用ELISA检测抗体滴度并用免疫细胞化学的方法检测此抗体对肝癌细胞中天然Foxm1b蛋白的识别。…”
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环尾狐猴源弓形虫的检测与鉴定
Published 2016-01-01“…用一步法PCR自6份样本中均扩增出529 bp目的片段,测序和Blast分析显示,该目的片段与GenBank中登录号为KC607824和DQ779189的刚地弓形虫(<i>Toxoplasma gondii</i>)核苷酸序列相似性最高,均为98.7%; 与国际标准强毒虫株RH的核苷酸相似性为98.5%。…”
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2798
Factor VIII Intron 22 Inversion in Severe Hemophilia A Patients in Palestine
Published 2020-01-01“…A cohort of 77 HA individuals including 5 carrier females from 52 unrelated families registered at governmental hemophilia centers in the West Bank area of Palestine was investigated. The demographic data and the clinical history were retrieved from medical files. …”
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Exploring the path to optimal diabetes care by unravelling the contextual factors affecting access, utilisation, and quality of primary health care in West Africa: A scoping review...
Published 2024-01-01“…Over 90% of cases are Type 2 diabetes (IDF, World Bank). As diabetes is ambulatory care sensitive, good primary care is crucial to reduce complications and mortality. …”
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