Next-generation sequencing and immuno-informatics for designing a multi-epitope vaccine against HSV-1-induced uveitis by He Cao, Zhi Cao, Yue Han, Jing Shan

“…This study aims to design a novel multi-epitope vaccine targeting HSV-1 glycoproteins B, C, D, H, and L using an immuno-informatics approach, which are essential for viral entry and pathogenesis.MethodsThe study identified epitopes for CD8+ T cells, CD4+ T cells, and B cells within the target glycoproteins. These epitopes were systematically evaluated for conservancy, immunogenicity, non-allergenicity, non-glycosylated regions, and binding affinities. …”
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Enrichment of Cancer‐Associated Fibroblasts, Macrophages, and Up‐Regulated TNF‐α Signaling in the Tumor Microenvironment of CMS4 Colorectal Peritoneal Metastasis by Eirik Høye, Chakravarthi Kanduri, Annette Torgunrud, Susanne Lorenz, Bjørn Edwin, Stein G. Larsen, Åsmund A. Fretland, Vegar J. Dagenborg, Kjersti Flatmark, Christin Lund‐Andersen

“…Results The cell type fractions in the TME were relatively similar between the metastatic sites, except for cancer‐associated fibroblasts (CAFs), B cells, endothelial cells, and CD4+ T cells. Notably, PM showed enrichment for CAFs and endothelial cells, consistent with distinct pathways associated with metastatic growth and progression in the peritoneal cavity. …”
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The Effect of Supplementation of Fish Protein Hydrolysate to the BSF-Based Aquafeed on the Growth, Survival, Fatty Acids, and Histopathology of Juvenile Lobster (Panulirus ornatus) by Ishaaq Saputra, Yih Nin Lee, Ravi Fotedar

“…However, the hepatopancreas histopathology indicated the presence of B-cells and R-cells in the juvenile lobsters fed with FPH-supplemented feeds. …”
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Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization by Qi Sun, Xin-Yu Nan, Hui Wang, Shuo Pan, Gang Ji, Ya-Feng Guo, Ya-Heng Zhao, Gao-Cen Li, Shao-Shi Guo, Lu-Feng Lin, Yu-Jie Jin, Xue Li Zhang, Chang-Cheng Liu, Guo-Bin Liu

“…MicroCT analysis was conducted to assess changes in the microstructure of subchondral bone trabeculae, and Western blot was performed to measure the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway and markers of M1 polarization in macrophages.ResultsPD significantly delays the progression of osteoarthritis induced by ACLT, effectively inhibits IL-1β-induced joint inflammation, bone metabolic remodeling and extracellular matrix degradation. …”
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Palmitoylation-related gene ZDHHC22 as a potential diagnostic and immunomodulatory target in Alzheimer’s disease: insights from machine learning analyses and WGCNA by Sanying Mao, Xiyao Zhao, Lei Wang, Yilong Man, Kaiyuan Li

“…Immune cell infiltration analysis revealed significant associations between ZDHHC22 expression and the infiltration of several immune cell types, including naïve B cells, CD8 + T cells, and M1 macrophages. In addition, 25 miRNAs and 55 lncRNAs were predicted to potentially target ZDHHC22, forming the basis for a lncRNA–miRNA–mRNA ceRNA network. …”
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Therapeutic Role of Protein Tyrosine Phosphatase 1B in Parkinson’s Disease via Antineuroinflammation and Neuroprotection In Vitro and In Vivo by Chien-Wei Feng, Nan-Fu Chen, Te-Fu Chan, Wu-Fu Chen

“…It could significantly attenuate the interferon-gamma-induced upregulation of proinflammatory cytokines, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). It enhanced M2 type microglia markers, such as arginase-1 and Ym-1 in BV2 murine microglial cells. …”
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Multiple machine learning-based integrations of multi-omics data to identify molecular subtypes and construct a prognostic model for HNSCC by Xiaoqin Luo, Chao Li, Gang Qin

“…The PRG-based signature stratified patients into low- and high-risk groups, with the low-risk group showing prolonged survival, enhanced immune cell infiltration (B cells, T cells, monocytes), and activated immune functions (cytolytic activity, T cell co-stimulation). …”
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Effects of B-Blockers on the Sympathetic and Cytokines Storms in Covid-19. by Al-kuraishy, Hayder M., Al-Gareeb, Ali Ismail, Mostafa-Hedeab, Gomaa, Keneth Iceland, Kasozi, Zirintunda, Gerald, Aslam, Akhmed, Allahyani, Mamdouh, Welburn, Susan Christina, Batiha, Gaber El-Saber

“…The potential mechanisms of SS in Covid-19 are through different pathways such as hypoxia, which activate the central sympathetic center through carotid bodies chemosensory input and induced proinflammatory cytokines, which cross the blood-brain barrier and activation of the sympathetic center. b2-receptors signaling pathway play a crucial role in the production of pro-inflammatory cytokines, macrophage activation, and B-cells for the production of antibodies with inflammation exacerbation. b-blockers have anti-inflammatory effects through reduction release of pro-inflammatory cytokines with inhibition of NF-kB. …”
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Increased Fas and Bcl-2 Expression on Peripheral Blood T and B Lymphocytes from Juvenile-Onset Systemic Lupus Erythematosus, but not from Juvenile Rheumatoid Arthritis and Juvenile... by Bernadete L. Liphaus, Maria H. B. Kiss, Solange Carrasco, Claudia Goldenstein-Schainberg

“…Otherwise, Fas expression on T and B cells from JRA and JDM patients were similar to healthy controls. …”
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HTR1D regulates the PI3K/Akt signaling pathway to impact hepatocellular carcinoma development and resistance to sorafenib by Yingai Zhang, Yuting Zhang, Shuai Zhou, Mujeeb Ur Rehman, Fankai Lin, Jianquan Zhang, Hailong Zhou

“…Then the siRNA was transfected into Huh-7 and Hep3B cells, and the cell proliferation ability, colony formation ability, migration and invasion ability were detected with or without sorafenib. …”
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Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis by Wei Shi, Dong Li, Qian Xu, Kai Zhang, Xinyu Liang, Hui Li, Zhijun Li, Huafeng Zhang

“…Results After integrating the 9 samples, 14 cell types were identified: B cells, Mesenchymal stem cells, Osteoblasts, Endothelial cells, Monocytes, T cells, NK cells, Fibroblasts, Macrophages, Common myeloid progenitors, Chondrocytes, Myelocytes, Osteoclasts, and Pericytes. …”
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Proliferating CLL cells express high levels of CXCR4 and CD5 by Daniel Friedman, Drshika P. Mehtani, Jennifer B. Vidler, Piers E. M. Patten, Robbert Hoogeboom

“…Abstract Chronic lymphocytic leukemia (CLL) is an incurable progressive malignancy of CD5+ B cells with a birth rate between 0.1% and 1% of the entire clone per day. …”
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The Efficacy of Processing Strategies on the Gastroprotective Potentiality of Chenopodium quinoa Seeds by Abdalbasit Adam Mariod, Suzy Munir Salama

“…Gene expression was performed for gastric tumor necrosis factor alpha (TNF-α) and nuclear factor kappa of B cells (NF-kB). QB and QG recorded the highest DPPH scavengers compared to QF and QP. …”
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Lung-delivered IL-10 therapy elicits beneficial effects via immune modulation in organic dust exposure-induced lung inflammation by Aaron D. Schwab, Todd A. Wyatt, Amy J. Nelson, Angela Gleason, Rohit Gaurav, Debra J. Romberger, Jill A. Poole

“…IL-10 therapy reduced ODE-associated expression of antigen presentation (MHC Class II, CD80, CD86) and inflammatory (Ly6C) markers and increased anti-inflammatory CD206 expression on CD11cintCD11b+ cells. ODE (12.5%, 25%)-induced lung pathology was also reduced with IL-10 therapy. …”
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Suppression of Proinflammatory Cytokines and Mediators in LPS-Induced RAW 264.7 Macrophages by Stem Extract of Alternanthera sessilis via the Inhibition of the NF-κB Pathway by Katyakyini Muniandy, Sivapragasam Gothai, Khaleel M. H. Badran, S. Suresh Kumar, Norhaizan Mohd Esa, Palanisamy Arulselvan

“…Inflammation is a natural process of the innate immune system, accompanied with the increase in the level of proinflammatory mediators, for example, nitric oxide (NO) and prostaglandin (PGE2); cytokines such as interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor alpha (TNFα); and enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) via the activation and nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit p65 due to the phosphorylation of inhibitory protein, IκBα. …”
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Neuroprotective Effects of Collagen-Glycosaminoglycan Matrix Implantation following Surgical Brain Injury by Jia-Hui Chen, Wei-Cherng Hsu, Kuo-Feng Huang, Chih-Huang Hung

“…Proinflammatory (tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells)) and anti-inflammatory (IL-10 and granulocyte-macrophage colony-stimulating factor (GMCSF)) cytokine expression was evaluated by enzyme-linked immunosorbent assays. …”
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MARCHF8-mediated ubiquitination via TGFBI regulates NF-κB dependent inflammatory responses and ECM degradation in intervertebral disc degeneration. by Xingpeng Zhang, Guang Li, Fang Tan, Tao Yu, Chengping Xu, Kai Li, Feng Zhang, Meiyan Zhang, Jian Wang

“…<h4>Objective</h4>Identify key modules associated with IDD and understand the impact of TGFBI on nucleus pulposus (NP) cell behavior, extracellular matrix (ECM)-related proteins, and the Nuclear Factor kappa-light-chain-enhancer of Activated B cells (NF-κB) signaling pathway.<h4>Methods</h4>The GSE146904 dataset underwent Weighted Gene Co-Expression Network Analysis (WGCNA) for key module identification and Differentially Expressed Genes (DEGs) screening. …”
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The transcription factor HMGB2 indirectly regulates APRIL expression and Gd-IgA1 production in patients with IgA nephropathy by Huijuan Tian, Yaling Zhai, Shuaigang Sun, Wenhui Zhang, Zhanzheng Zhao

“…RNA interference and coimmunoprecipitation (Co-IP) were used to verify the relationships among HMGB2, high mobility group AT-hook protein 1 (HMGA1), and APRIL.Results HMGB2 expression was greater in IgAN patients than in HCs and was positively associated with APRIL expression in B cells. DNA pull-down and protein profiling revealed that HMGB2 and HMGA1 bound to the promoter region of the APRIL gene. …”
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Immune microenvironment spatial landscapes of tertiary lymphoid structures in gastric cancer by Yi Xie, Haoxin Peng, Yajie Hu, Keren Jia, Jiajia Yuan, Dan Liu, Yanyan Li, Xujiao Feng, Jian Li, Xiaotian Zhang, Yu Sun, Lin Shen, Yang Chen

“…ST analysis confirmed that T and B cells co-localise within TLS, enhancing immune response activation compared to cancer nests and exerting a strong anti-tumour effect. …”
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IgG4-mediated M2 macrophage polarization in tertiary lymphoid structures of esophageal cancer: implications for immunosuppression by Hui Wang, Hui Wang, Jirui Li, Yinghai Wang, Yang Chen, Yang Chen, Weifeng Zhang, Xinyan Pan, Xinyan Pan, Chanjuan Su, Ziteng Li, Li Wang, Li Wang, Jiang Gu, Jiang Gu

“…This study aims to analyze the distribution characteristics of IgG4-expressing cells, regulatory T cells (Tregs), and M2-type macrophages as well as to elucidate the relationship between IgG4 and the polarization of M2 macrophages within TLS in esophageal cancer.ObjectTo elucidate the distribution of IgG4, Treg cells, and M2 macrophages in TLS and to assess the impact of IgG4 on macrophage polarization.MethodsEsophageal cancer tissue were analyzed with multiplex immunofluorescence to determine the spatial distribution and density of B cells, T cells, and their subtypes. The relationship between IgG4 and CD8+ T cells in TLS, along with interleukin-10 (IL-10) expression and Treg presence, was studied. …”
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