Extracellular viral microRNAs as biomarkers of virus infection in human cells by Cheryl Chan, Joanne Xin Yi Loh, Wei-Xiang Sin, Denise Bei Lin Teo, Nicholas Kwan Zen Tan, Chandramouli Nagarajan, Yunxin Chen, Francesca Lorraine Wei Inng Lim, Michael E. Birnbaum, Rohan B.H. Williams, Stacy L. Springs

“…Viral exmiR profiling of spent media from EBV- and HHV-8-reactivated B cell models revealed specific signatures of elevated EBV miR-BHRF1-2-3p and HHV-8 miR-K12-10a-3p, miR-K12-10b, and miR-K12-12-3p, respectively, during virus reactivation. …”
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Genetic characteristics involving the PD-1/PD-L1/L2 and CD73/A2aR axes and the immunosuppressive microenvironment in DLBCL by Tingting Zhang, Zhenzhen Zhang, Xiubao Ren, Jin He, Zhengzi Qian, Bin Meng, Lei Jiao, Hengqi Liu, Lanfang Li, Lihua Qiu, Shiyong Zhou, Wenchen Gong, Huilai Zhang, Xianhuo Wang

“…The genetic characteristics of these immune checkpoints need to be further investigated in diffuse large B-cell lymphoma (DLBCL).Methods We performed whole-exome sequencing/targeted deep sequencing to investigate the genetic characteristics of PD-1/PD-L1/L2 and CD73/A2aR. …”
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Characteristics and clinical significance of immune cells in omental milky spots of patients with gastric cancer by Yasunobu Mano, Yasunobu Mano, Yuka Igarashi, Yuka Igarashi, Keisuke Komori, Keisuke Komori, Itaru Hashimoto, Itaru Hashimoto, Hayato Watanabe, Kosuke Takahashi, Kazuki Kano, Hirohito Fujikawa, Takanobu Yamada, Hidetomo Himuro, Hidetomo Himuro, Taku Kouro, Taku Kouro, Feifei Wei, Feifei Wei, Kayoko Tsuji, Kayoko Tsuji, Shun Horaguchi, Shun Horaguchi, Shun Horaguchi, Mitsuru Komahashi, Mitsuru Komahashi, Mitsuru Komahashi, Takashi Oshima, Tetsuro Sasada, Tetsuro Sasada

“…Cytometric analysis revealed significantly more T and B cells and fewer neutrophils in G3. Accordingly, the immune microenvironment in omental milky spots may vary depending on the stage of gastric cancer progression. …”
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CD40-TRAF6 inhibition suppresses cardiovascular inflammation, oxidative stress and functional complications in a mouse model of arterial hypertension by Lea Strohm, Henning Ubbens, Dominika Mihalikova, Alexander Czarnowski, Paul Stamm, Michael Molitor, Stefanie Finger, Matthias Oelze, Dorothee Atzler, Philip Wenzel, Philipp Lurz, Thomas Münzel, Christian Weber, Esther Lutgens, Andreas Daiber, Steffen Daub

“…Therefore, we conclude that targeting CD40 directly on adipocytes, B-cells, T-cells, or CD40L on platelets is not a promising target to prevent hypertension complications.In summary, TRAF6 inhibition but not adipocyte, B-cell, or T-cell-specific CD40 or platelet-specific CD40L deficiency reduces pathophysiological vascular inflammation in hypertensive mice, suggesting TRAF6 inhibition as a potential therapeutic target in hypertensive patients.…”
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Effect of the Iridoid-rich Fraction from <italic>Valeriana jatamansi</italic> Jones on Oxidative Stress in Rats with Spinal Cord Injury by Jiaojuan HUANG, Wenchun WANG, Deqi XIONG, Hao LU, Wu XIANG, Xiaohua ZHAO, Ruihan ZHOU, Anren ZHANG

“…The rats were sacrificed on third days after administration, and the contents of oxidative stress indexes superoxide dismutase(SOD)and malondialdehyde(MDA)in serum were detected by enzyme linked immunosorbent assay(ELISA); the related biochemical kit was used to test glutathione peroxidase(GSH-PX)and catalase(CAT)viability of the spinal cord tissue; the expression levels of apoptosis-related proteins of Caspase-3, B cell lymphoma(Bcl-2)associated X protein(Bax), and Bcl-2 were detected by Western blot; and the Hematoxylin/eosin(HE)staining was used to observe the pathological changes of the spinal cord tissue at the perilesional area; and Terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL)was used to observe the apoptosis of nerve cells of the spinal cord tissue at the perilesional area.Results:The results of ELISA showed that compared with the sham-operated group, the SOD content in the serum of the model group decreased, and the MDA content increased; compared with the model group, the SOD content in the serum of the treatment group with iridoid-rich fraction from <italic>Valeriana jatamansi</italic> Jones increased significantly(<italic>P</italic>&lt; 0.05), and the MDA content decreased significantly(<italic>P</italic>&lt; 0.01). …”
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Beyond Dermatological Findings: Multisystem Involvement in Prolidase Deficiency by Ezgi Yalcin Gungoren, Zeynep Meric, Asena Pinar Sefer, Asuman Deveci Ozkan, Salim Can, Royala Babayeva, Nurhan Kasap, Ercan Nain, Esra Ozek Yucel, Ayca Kiykim, Sevgi Bilgic-Eltan, Ayse Deniz Yucelten, Elif Karakoc-Aydiner, Ahmet Ozen, Safa Baris

“…P2 had diffuse large B-cell lymphoma, resulting in early death. Interestingly, P1 and P2 experienced opportunistic infections such as cytomegalovirus, Epstein-Barr virus, and Pneumocystis jirovecii. …”
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Study on the protective effect of mitochonic acid-5 on septic acute kidney injury and the underlying mechanism by Li Peng-yan, Yan miao, Yang Ding-ping

“…方法细胞实验：将肾小管细胞分为正常组、MA-5（10 µmol/L）组、LPS（10 mg/L）刺激组和LPS+MA-5治疗组，通过细胞计数试剂盒8法评估细胞活力、流式细胞术评估细胞凋亡率；活性氧（reactive oxygen species，ROS）检测试剂盒检测ROS含量；三磷酸腺苷（adenosine triphosphate，ATP）检测试剂盒检测ATP水平；蛋白质印迹法（western blot，Wb）检测沉默信息调节因子3（silence information regulator 3，SIRT3）蛋白、线粒体动力学相关蛋白［动力相关蛋白1（dynamin-related protein 1，DRP1）、视神经萎缩因子1（optic atrophy 1，OPA1）］和细胞凋亡相关蛋白［B细胞淋巴瘤2（B-cell lymphoma-2，Bcl-2）、Bcl-2相关X蛋白（Bcl-2 associated X protein，Bax）］的表达水平。…”
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Trivalent recombinant protein vaccine induces cross-neutralization against XBB lineage and JN.1 subvariants: preclinical and phase 1 clinical trials by Jingyun Yang, Weiqi Hong, Huashan Shi, Cai He, Hong Lei, Yanan Zhou, Hao Yang, Aqu Alu, Zimin Chen, Yun Yang, Wenhai Yu, Cong Tang, Junbin Wang, Bai Li, Qing Huang, Jiong Li, Li Yang, Wei Wang, Guobo Shen, Jinliang Yang, Zhiwei Zhao, Xiangrong Song, Zhaoming Su, Yuquan Wei, Qiangming Sun, Shuaiyao Lu, Zhenling Wang, Youchun Wang, Guangwen Lu, Weimin Li, Xiawei Wei

“…It elicits measurable antigen-specific T cell responses, germinal center B cell responses, and T follicular helper responses, effectively protecting against live Omicron XBB.1.16 challenges. …”
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Development of a recombinant infectious bronchitis virus vaccine expressing infectious laryngotracheitis virus multiple epitopes by Guanming Shao, Jun Fu, Yun Pan, Shiying Gong, Chaoyi Song, Sheng Chen, Keyu Feng, Xinheng Zhang, Qingmei Xie

“…In this study, we designed a multi-epitope cassette (gD-T/B) containing multiple T and B cell epitopes of ILTV gD protein. The genetic stability of the full-length gD gene and the gD-T/B multi-epitope cassette replacing non-essential genes in IBV was systematically analyzed. …”
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The age-adjusted international prognostic index 2 (A-FLIPI2) for elderly patients with follicular lymphoma by Jiesong Wang, Junlei Jia, Jingwei Yu, Jing Liu, Meng Gao, Hengqi Liu, Lanfang Li, Lihua Qiu, Shiyong Zhou, Bin Meng, Wenchen Gong, Zhengzi Qian, Xianhuo Wang, Huilai Zhang

“…Follicular lymphoma (FL) is a common B-cell lymphoma and typically affects the elderly population. …”
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Clinical impact of [18F]FDG-PET/CT in ARI0002h treatment, a CAR-T against BCMA for relapsed/refractory multiple myeloma by Inés Zugasti, Marta Tormo-Ratera, Aina Oliver-Caldés, Juan Carlos Soler-Perromat, Verónica González-Calle, David F. Moreno, Valentín Cabañas, Nieves López-Muñoz, Álvaro Bartolomé-Solanas, Marta Español-Rego, Juan Luis Reguera-Ortega, Laura Rosiñol, Lucía López-Corral, Natalia Tovar, Luis Gerardo Rodríguez-Lobato, Rosa Maria Alvarez Perez, Sara Varea, Eulàlia Olesti, Adolfo Gomez-Grande, Laura Frutos, Pilar Tamayo, Manel Juan, José M. Moraleda, Álvaro Urbano-Ispizua, E. Azucena González-Navarro, Joaquín Martínez-López, Maria-Victoria Mateos, Xavier Tomás, Xavier Setoain, Carlos Fernández de Larrea

“…This study included 63 patients with relapsed/refractory MM treated either in the CARTBCMA-HCB-01 clinical trial (ARI0002h; academic B-cell maturation antigen [BCMA]–targeted CAR T-cell therapy) or in compassionate use. …”
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Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy? by Agnieszka Pozdzik, Ingrid Beukinga, Chunyan Gu-Trantien, Karen Willard-Gallo, Joëlle Nortier, Olivier Pradier

“…RTX induced complete disappearance of CD19+ B cells, plasmablasts, and memory B cells as soon as day 15. …”
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Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes by Jue Lin, Joshua Cheon, Rashida Brown, Michael Coccia, Eli Puterman, Kirstin Aschbacher, Elizabeth Sinclair, Elissa Epel, Elizabeth H. Blackburn

“…Additionally, PBMC TL change was also correlated with those of all three T cell types, and B cells. The rate of shortening for B cells was significantly greater than for the three T cell types. …”
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The Increase of Circulating PD-1- and PD-L1-Expressing Lymphocytes in Endometriosis: Correlation with Clinical and Laboratory Parameters by Monika Walankiewicz, Ewelina Grywalska, Grzegorz Polak, Izabela Korona-Glowniak, Elzbieta Witt, Agata Surdacka, Jan Kotarski, Jacek Rolinski

“…Alteration of the PD-1/PD-L1 axis may contribute to the pathogenesis of endometriosis, as patients with advanced disease are characterized by higher frequencies of PD-1-positive T and B cells. Expression of PD-1 and PD-L1 on T and B cells could represent the hallmark of immune system reaction to chronic antigenic exposition in patients with endometriosis.…”
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Impact on CD4+ CD25High-CD127low regulatory T (Treg) cells of neoadjuvant therapy for rectal cancer patients by Liu Maoxi, Liu Haiyi

“…Treg, CD4+ T, CD8+ T, NK, B cells, and CD4+/CD8+ ratio in peripheral blood before and after neoadjuvant therapy were measured by flow cytometry. …”
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Single-cell profiling transcriptomic reveals cellular heterogeneity and cellular crosstalk in breast cancer lymphatic node, bone, and brain metastases by Longyu Zhu, Miaomiao Liu, Yuguang Shang, Jingge Cheng, Hongye Zhao, Jun Zhang, Dongxing Shen

“…We identified five T cells (T memory, CD8 + T cells, regulatory T cells, natural killer cells, CD4 + T cells), three B cells (naïve B cells, memory B cells, plasma B cells), and five cancer-associated fibroblasts (CAFs) subpopulations (Smooth muscle cells (SMC), pericyte, antigen-presenting CAFs (apCAFs), proliferative CAFs (pCAFs), and matrix CAFs (mCAFs)). …”
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Effect of Tumor Necrosis Factor Family Member LIGHT (TNFSF14) on the Activation of Basophils and Eosinophils Interacting with Bronchial Epithelial Cells by Huai Na Qiu, Chun Kwok Wong, Jie Dong, Christopher Wai-Kei Lam, Zhe Cai

“…Results showed that LIGHT could significantly promote intercellular adhesion, cell surface expression of intercellular adhesion molecule-1, release of airway remodeling-related IL-6, CXCL8, and MMP-9 from BEAS-2B cells upon interaction with basophils/eosinophils, probably via the intercellular interaction, cell surface receptors HVEM and LTβR on BEAS-2B cells, and extracellular signal-regulated kinase, p38 mitogen activated protein kinase, and NF-κB signaling pathways. …”
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BLINATUMOMAB IN THE THERAPY OF ACUTE B-LYMPHOID LEUKEMIA by Ugo Testa, Elvira Pelosi, Germana Castelli, Patrizia Chiusolo

“… Blinatumomab, a CD19-CD3 bispecific T cell engager (BiTE), has two recombinant single-chain variable fragments that temporarily link CD3+ T cells and CD19+ B cells, leading to the T cell-mediated lysis of neoplastic B cells. …”
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From fibrosis to granuloma: drug induced systemic sarcoidosis-like reaction after rituximab in a patient with primary Sjögren’s syndrome by Rui Rua Coelho, Sara Xavier Pires, José Ricardo Brandão, Inês Furtado

“…There has been much speculation about the potential role of B cells in sarcoidosis. Findings show a decrease of B memory cells and an increase in naïve and active subsets of regulatory B cells in sarcoidosis patients, which resembles the repopulation with naïve B cells after treatment with rituximab. …”
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New approach to generating of human monoclonal antibodies specific to the proteolytic domain of botulinum neurotoxin A by Marina Vladimirovna Silkina, Alena Sergeevna Kartseva, Alena Konstantinovna Riabko, Mariia Aleksandrovna Makarova, Metkhun Madibronovich Rogozin, Yana Olegovna Romanenko, Igor Georgievich Shemyakin, Ivan Alekseevich Dyatlov, Victoria Valerievna Firstova

“…Methods: Plasmablasts and activated memory B-cells were isolated from whole blood collected 7 days after BoNT/A injection and sorted by flow cytometry. …”
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