Ames Assay Transferred from the Microtiter Plate to the Planar Assay Format by Katharina Schmidtmann, Johanna Lemme, Gertrud E. Morlock

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How Controls Improve Diagnostic Assay Performance: Hitchhiker's Guide to Diagnostic Assay Controls by Frank N. Martin, Ozgur Batuman, Douglas G. Luster, Timothy D. Miles, Yazmín Rivera, Poonam Sharma, David Geiser, Kitty Cardwell

Subjects: “…diagnostic assay…”
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Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays by Alexei V. Timofeev, Ksenia A. Gorst, Valentin Y. Uvarov, Ekaterina A. Pronina, Alisa V. Vitebskaya, Anastasiya V. Popovich, Anatoliy N. Tiulpakov, Natalia A. Zubkova, Olesya A. Gioeva, Yulia V. Tikhonovich, Alexander V. Ilin, Oleg Y. Latyshev, Goar F. Okminjan, Ksenia L. Kabolova, Julianna A. Kanokova, Leokadia Y. Zhuleva, Igor E. Koltunov, Elena E. Petriaikina, Irina G. Rybkina, Irina V. Garyaeva, Salome L. Guguchia, Angelina B. Shimarova, Artiom V. Bullikh, Irina G. Kolomina, Evgenia A. Evsyukova, Sergei S. Bukin, Natalia N. Egarmina, Natalia Y. Arbatskaya, Elina Y. Yanovskaya, Vladimir I. Popenko, Alexander A. Arkhipkin

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Guideline for anticancer assays in cells by Fei‐Fei Ma

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Partitioning Indicator Displacement Assay by Vincent Tocci, Emily Shiel, Huanhuan Zhou, Shu Liu, Tracey Bell, Steven Lenhert

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Microtiter Immunocytochemical ELISA Assay by Suzanne M. de la Monte, Neema Ganju, Jack R. Wands

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Methodology for Wetland Seedbank Assays by Carrie Reinhardt Adams, Nancy M. Steigerwalt

“…ENH1090/EP354: Methodology for Wetland Seedbank Assays (ufl.edu) …”
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Methodology for Wetland Seedbank Assays by Carrie Reinhardt Adams, Nancy M. Steigerwalt

“…ENH1090/EP354: Methodology for Wetland Seedbank Assays (ufl.edu) …”
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Mitigation of the effect of variability in digital PCR assays through use of duplexed reference assays for normalization by Sherry J Coulter

“…The initial method of representing data as copies per microliter produced inconsistent results and made inter-assay comparisons difficult. Normalizing copies to amount of nucleic acid gives more uniform results, but factors influencing the effective concentration of nucleic acid in the final digital PCR assay must be considered. …”
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AssayBLAST: A Bioinformatic Tool for <i>In Silico</i> Analysis of Molecular Multiparameter Assays by Maximilian Collatz, Sascha D. Braun, Martin Reinicke, Elke Müller, Stefan Monecke, Ralf Ehricht

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Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool by Bing Wang, Jincui Gu, Boyi Chen, Chengfeng Xu, Hailing Zheng, Zhiqin Peng, Yang Zhou, Zhiwen Hu

“…Then, an enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-labelled immunochromatographic strip (ICS) were developed to qualitatively identify the corresponding protein in ancient wool samples unearthed from Kazakhstan and China. …”
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Atezolizumab neutralizing assay development: a novel way of evaluating assay cutpoint after sample pretreatment by Rachel Melendez, Jane Ruppel, Cecilia Leddy, Jenny Yang, Ann Brady, Yenny Webb-Vargas, Jochen Brumm, Lynn Kamen, Yuan Song

“…The resulting assay was successfully validated and applied to sample analysis from multiple clinical trials.…”
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An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK) by Trezze P. Nguyen, Shangze Lyu, Yang Liu

“…Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity. …”
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Development and Characterization of the NanoOrange® Protein Quantitation Assay: A Fluorescence-Based Assay of Proteins in Solution by Laurie J. Jones, Richard P. Haugland, Victoria L. Singer

“…The protein-to-protein variability of the NanoOrange assay was comparable to those of standard assays, including Lowry, bicinchoninic acid, and Bradford procedures. …”
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Clinical validation and evaluation of the EasyNAT Malaria assay and the Alethia Malaria assay in a non-endemic setting: rapid and sensitive assays for detecting Plasmodium spp. in returning travellers by Charlotte van der Veer, Julian Apako, Anja Sonneveld-Hendriks, Annemarie Kaak, Cindy Arias-Claro Handgraaf, Erik Schaftenaar, Guido J.H. Bastiaens, Jacky Flipse

“…This study assessed the analytical and clinical performance of the EasyNAT Malaria cross-priming amplification assay and the Alethia Malaria loop-mediated isothermal amplification assay in screening for Plasmodium spp. in febrile patients with recent travel history to malaria endemic regions.Using the composite microbial reference, the overall sensitivity and specificity were: 100 % and 97.5 % for the EasyNAT Malaria assay and 97.8 % and 98.3 % for the Alethia Malaria assay, respectively. …”
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Quantitative assay for ciprofloxacin and enrofloxacin formulations by Saundria Moed, Mackenzie Hall, Norman Lee, Clarissa Prazeres da Costa, Ernst K Rødland, Atena I Shemirani, Katie Clifford, Darash Desai, Muhammad H Zaman

“…In this assay, cipro is dissolved in deionized water and mixed with palladium(II) and methyl cellulose. …”
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Rapid Bioluminescent Antibiotic Susceptibility Assay by V. G. Frundzhyan, N. N. Ugarova, L. A. Blatun, R. P. Terekhova, E. V. Rusanova

“…Satisfactory correlation (R2≥88%) of the results of the bioluminescent assay and the assay with the disk diffusion method and the method of serial dilutions was observed.…”
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An agarose spot assay for chemotactic invasion by Helen L. Wiggins, Joshua Z. Rappoport

“…Here we describe a novel chemotaxis assay based upon the invasion of cells into agarose spots into which chemoattractants are suspended. …”
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Pitfalls and other issues with the MTT assay by David Kessel

“…While the colorimetric assay commonly referred to as ‘MTT’ does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay. …”
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Optimization of Fluorometric Assay for Ozone in Solution by Hiromi Nagano, Gotaro Shiota, Hidetoshi Arakawa

“…We conclude that this assay will be useful for quantifying ozone in solution.…”
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