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Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro
Published 2025-01-01“…In the presence of 1 mM glycine, we adjusted the osmolality of the culture medium from low to high (290 mOsM for the first 22 h and 320 mOsM thereafter) by adding raffinose, which cannot be used by animal cells, in a culture stage-dependent manner.ResultsStage-dependent adjustment of simplified medium PZM-3 osmolarity (290 mOsM for the first 22 h and 320 mOsM thereafter) in the presence of 1 mM glycine significantly improved the quality of porcine oocyte maturation in vitro, manifested by the oocyte maturation rate, functional mitochondrial distribution and activity, the transcript levels of glycolysis genes in granulosa cells, and subsequent embryonic developmental ability and ROS levels.ConclusionOur study demonstrates that optimizing cell volume regulation can further enhance the developmental potential of oocytes cultured in vitro.…”
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2203
L’activité communicationnelle enseignante lors des discussions à visée philosophique
Published 2016-01-01“…The paper deals with animation in Collaborative philosophical Inquiry. Like psychologists ergonomists, we develop that to design training on communicational activity (animation in CPI in this case), it’s necessary to understand this activity through the study of its accomplishment. …”
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Effects of reproductive technologies and SPF status on some physiological and behavioral characteristics in rats with arterial hypertension (ISIAH strain)
Published 2015-12-01“…Modern standards of Laboratory Animal Science include working with laboratory animals of high quality, in particular, with specific pathogen free (SPF) mice and rats. …”
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2206
Deciphering Pectin: A Comprehensive Overview of Its Origins, Processing, and Promising Utility
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When is synthetic sufficient? Ethical considerations and alternatives in simulation-based ultrasound education
Published 2025-01-01“…Although research indicates that animal models yield comparable educational outcomes to synthetic models, animal models continue to be preferred in surgical and medical training. …”
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Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens
Published 2025-01-01“…At week 14 after prime vaccination of the animals, skin tests, IFN-γ release assay, and antibody detection assays were performed. …”
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Corrigendum: Specific detection of duck adeno-associated virus using a TaqMan-based real-time PCR assay
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Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats
Published 2025-06-01“…Gene expression and post-transcriptional regulation are key mechanisms affecting lactation performance in dairy animals. However, the difficulty of obtaining mammary gland tissue samples from lactating animals has significantly impeded lactation research. …”
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2219
Effect of Praziquantel on Preventing Delayed Infection of <i>Schistosoma japonicum</i> in Buffaloes and Goats
Published 2024-12-01“…Schistosomiasis, caused by <i>Schistosoma japonicum</i>, continues to pose a major public health threat in East Asia, with an estimated 71 million people at risk of infection. Domestic animals, especially buffaloes and goats, serve as important reservoir hosts, facilitating the transmission of the parasite to humans. …”
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