Organoids and spheroids: advanced in vitro models for liver cancer research by Mirella Pastore, Alessia Giachi, Elena Spínola-Lasso, Fabio Marra, Chiara Raggi

“…While traditional in vivo animal models have played a vital role in liver cancer research, ethical concerns and the demand for more human-relevant systems have driven the development of advanced in vitro models. …”
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Breakfast Protein Source Does Not Influence Postprandial Appetite Response and Food Intake in Normal Weight and Overweight Young Women by Christina M. Crowder, Brianna L. Neumann, Jamie I. Baum

“…Our objective was to determine if protein source (animal protein (AP) versus plant protein (PP)) influences postprandial metabolic response in participants consuming a high protein breakfast (~30% energy from protein). …”
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Modeled sustainability impacts of increasing pork consumption among adults in the United States by Zach Conrad, Zach Conrad, Vincent Repoulis, Catherine Zavela

“…IntroductionLittle is known about the role of pork in sustainable diet patterns, given that it is often aggregated with other animal proteins or not evaluated at all. To address this gap, this study modeled the sustainability impacts of replacing different protein foods with pork in a nationally representative sample of adults in the United States (US).MethodsData on dietary intake, greenhouse gas emissions (GHGE), cumulative energy demand, water scarcity footprint, land, pesticides, fertilizer nutrients, food prices, and diet quality were obtained from publicly available repositories. …”
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Gender-Specific Hippocampal Dysrhythmia and Aberrant Hippocampal and Cortical Excitability in the APPswePS1dE9 Model of Alzheimer’s Disease by Anna Papazoglou, Julien Soos, Andreas Lundt, Carola Wormuth, Varun Raj Ginde, Ralf Müller, Christina Henseler, Karl Broich, Kan Xie, Dan Ehninger, Britta Haenisch, Marco Weiergräber

“…In females, theta (θ) and gamma (γ) power alterations predominated in the inactive state suggesting a reduction in atropine-sensitive type II theta in APPswePS1dE9 animals. Gender-specific central dysrhythmia and network alterations in APPswePS1dE9 point to a functional role in behavioral and cognitive deficits and might serve as early biomarkers for AD in the future.…”
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Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne by Guang Zuo, Yidan Gao, Guangtong Lu, Ming Bu, Jun Liu, Juncha Zhang, Xisheng Fan, Hao Chen, Xuesong Wang, Yanfen She

“…Propionibacterium acnes was injected subcutaneously into the ears of rats to establish an animal model of acne. The auriculotherapy intervention in rats consisted of auricular bloodletting therapy (ABT), auricular point sticking (APS), or a combination of both (ABPS). …”
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Functional Metaplasticity of Hippocampal Schaffer Collateral-CA1 Synapses Is Reversed in Chronically Epileptic Rats by Mirko Rehberg, Timo Kirschstein, Xiati Guli, Steffen Müller, Marco Rohde, Denise Franz, Tursonjan Tokay, Rüdiger Köhling

“…As expected, memory acquisition in the behavioral task was significantly impaired both in pilocarpine-treated animals and in adult controls. Accordingly, these groups, without being tested in the behavioral training task, showed reduced CA1-LTP levels compared to untrained young controls. …”
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Effect of grape seed extract on β -catenin gene expression and hyperglycemia in rats induced by streptozotocin by Elif Gulbahce-Mutlu, Emine Arslan, Hilal Arikoglu

“…The water-soluble extracts were administered by gavage for four weeks and liver, pancreas and blood samples taken from animals at the end of treatment were used in the study.…”
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Experimental Evidence of Rapidly Decaying Environmental DNA Highlights Infection Risk from Two Major Amphibian Pathogens by Joseph D. Trafford, Trenton W. J. Garner, David J. Murrell, Julia J. Day

“…These pathogens spread internationally through live‐animal trade networks and have driven population declines, mass mortalities, and community collapse for a broad range of amphibian species. …”
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Renal Effects of Hyperbaric Oxygen Therapy in Patients with Diabetes Mellitus: A Retrospective Study by Martin Sedlacek, Nicole P. Harlan, Jay C. Buckey

“…The prolonged high oxygen level used in HBOT can produce oxidative stress, which may be harmful to the kidney. Animal experiments suggest HBOT does not harm renal function and may have an antiproteinuric effect, but little is known on the effect of HBOT in humans. …”
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Analysis of the cardiotoxic and myorelaxant effects of camphor on fish of the Nile tilapia species (Oreochromis niloticus) (Linnaeus 1758) by Clarissa Araújo da Paz, Suzane Maia de Oliveira, Maria Klara Otake Hamoy, Murilo Farias dos Santos, Anthony Lucas Gurgel do Amaral, Anara de Sousa Barbosa, Luciana Eiró-Quirino, Daércio José de Macedo Ribeiro Paixão, Luis André Luz Barbas, Dielly Catrina Favacho Lopes, Marcelo Ferreira Torres, Moisés Hamoy

“…However, the use of a concentration of 300 mg L−1 makes the cardiac and respiratory effects more evident, with changes in the tracing, which can harm the animal’s hemodynamics. However, lower concentrations (150 mg L−1) showed tachycardia and opercular hypermotility, demonstrating changes not compatible with anesthesia. …”
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Zearalenone exacerbates lipid metabolism disorders by promoting liver lipid droplet formation and disrupting gut microbiota by Xiao Han, Bingxin Huangfu, Tongxiao Xu, Kunlun Huang, Xiaoyun He

“…ZEA has the ability to bind to estrogen receptors of humans and animals and is an environmental endocrine disruptor that may interfere with glucose homeostasis and lipid metabolism. …”
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<i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model by Francisco Allysson Assis Ferreira Gadelha, Raquel Fragoso Pereira Cavalcanti, Cosmo Isaias Duvirgens Vieira, Joao Batista De Oliveira, Louíse Mangueira De Lima, Adriano Francisco Alves, Matheus Marley Bezerra Pessoa, Leônia Maria Batista, Naiara Naiana Dejani, Marcia Regina Piuvezam

“…<b>Methods</b>: Swiss mice were challenged with lipopolysaccharide and treated with HEM after 1, 24, and 48 h (five animals/group, three times). <b>Results</b>: The HEM-treated ALI mice presented a decrease in neutrophil migration in the bronchoalveolar lavage fluid (BALF), in the alveolar region, and in the blood, correlating to downregulation of CD18 expression. …”
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Structures of Astaxanthin and Their Consequences for Therapeutic Application by Tatas Hardo Panintingjati Brotosudarmo, Leenawaty Limantara, Edi Setiyono, Heriyanto

“…Astaxanthin, a xanthophyll carotenoid, is a secondary metabolite responsible for the red-orange color of a number of marine animals and microorganisms. There is mounting evidence that astaxanthin has powerful antioxidant, anti-inflammatory, and antiapoptotic activities. …”
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Toll-Like Receptor-4 Mediated Inflammation Is Involved in the Cardiometabolic Alterations Induced by Intermittent Hypoxia by Laureline Poulain, Vincent Richard, Patrick Lévy, Maurice Dematteis, Claire Arnaud

“…Lean adult male TLR4-deficient (TLR4−/−) mice and their controls (C57BL/6 mice) were exposed to either IH (FiO2 21-5%, 1 min cycle, 8 h/day) or air (normoxic mice) for 4 weeks. Animals were assessed at 1-week exposure for insulin tolerance test and after 4-week exposure for morphological and inflammatory changes of the epididymal fat and thoracic aorta. …”
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Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus by Tenggen Hu, Yuanshan Yu, Jijun Wu, Yujuan Xu, Gengsheng Xiao, Kejing An, Erna Li, Sentai Liao, Yuxiao Zou

“…Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus, a commensal bacterium presented a reduced level in T2DM mice. Animal experiments indicated that MLO 2-1 and L. murinus exhibited hypoglycemic activities. …”
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Detection of Francisellaceae and the differentiation of main European F. tularensis ssp. holarctica strains (Clades) by new designed qPCR assays by Kristin Köppen, Kerstin Rydzewski, Julia Zajac, Marwah Al-Senwi, Sema Evcimen, Darius Schulze, Daniela Jacob, Klaus Heuner

“…Conclusion Since the developed qPCRs are able to detect all genera of Francisellaceae tested, an easy and fast identification of opportunistic Francisella strains causing tularemia-like symptoms in humans or animals is possible now. The application of these qPCR assays will thus improve the capability for clinical diagnostics and molecular typing during epidemiological investigations.…”
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Selective Glucocorticoid Receptor (GR-II) Antagonist Reduces Body Weight Gain in Mice by Tomoko Asagami, Joseph K. Belanoff, Junya Azuma, Christine M. Blasey, Robin D. Clark, Philip S. Tsao

“…Previous research has shown that mifepristone can prevent and reverse weight gain in animals and human subjects taking antipsychotic medications. …”
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Personalized allele-specific antisense oligonucleotides for GNAO1-neurodevelopmental disorder by Inna Shomer, Nofar Mor, Shaul Raviv, Noga Budick-Harmelin, Tanya Matchevich, Sharon Avkin-Nachum, Yoach Rais, Rebecca Haffner-Krausz, Ariela Haimovich, Aviv Ziv, Reut Fluss, Bruria Ben-Ze’ev, Gali Heimer, Denis N. Silachev, Vladimir L. Katanaev, Dan Dominissini

“…Due to sequence similarity, the mouse can be treated with the selected ASO to test treatment efficacy in animal models, as shown in vitro using murine neural progenitor cells. …”
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Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role for CTGF in Human Diabetic Foot Ulcer Healing by F. R. Henshaw, P. Boughton, L. Lo, S. V. McLennan, S. M. Twigg

“…Granulation tissue from CTGF treatment in diabetic wounds showed collagen IV accumulation compared with nondiabetic animals. Wound α-smooth muscle actin was increased in CTGF treated diabetic wounds compared with untreated diabetic wounds, as was macrophage infiltration. …”
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Protective Effect of Opuntia dillenii (Ker Gawl.) Haw. Seed Oil on Gentamicin-Induced Nephrotoxicity: A Biochemical and Histological Analysis by Mohamed Bouhrim, Noureddine Bencheikh, Hamada Imtara, Nour Elhouda Daoudi, Hamza Mechchate, Hayat Ouassou, Loubna Kharchoufa, Mostafa Elachouri, Hassane Mekhfi, Abderrahim Ziyyat, Abdelkhaleq Legssyer, Mohammed Aziz, Mohamed Bnouham

“…The present study aims to investigate the protective effect of Opuntia dillenii seed oil against gentamicin-induced nephrotoxicity in rats. The animals (rats) were randomly divided into three groups (i) the normal control group treated only with distilled water (10 mL/kg), (ii) the gentamicin group treated with distilled water (10 mL/kg) and received an intraperitoneal injection of gentamicin (80 mg/kg), and (iii) the group treated with the Opuntia dillenii seed oil (2 mL/kg) and also received an intraperitoneal injection of gentamicin (80 mg/kg). …”
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