Low-Intensity Pulsed Ultrasound Modulates RhoA/ROCK Signaling of Rat Mandibular Bone Marrow Mesenchymal Stem Cells to Rescue Their Damaged Cytoskeletal Organization and Cell Biolog... by Rong Zhang, Zhaoling Wang, Guoxiong Zhu, Gaoyi Wu, Qingyuan Guo, Hongchen Liu, Lei Chen

“…In this study, the effects of LIPUS on cytoskeletal reorganization, cell viability, and osteogenic differentiation capacity of rat mandible-derived bone marrow mesenchymal stem cells (M-BMMSCs) induced by radiation were determined by immunofluorescence staining, CCK-8 cell proliferation assay, quantification of alkaline phosphatase (ALP) activity, alizarin red staining, and real-time RT-PCR, respectively. …”
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Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells by Weili Xu, Pan He, Shenghua He, Pengju Cui, Yaqing Mi, Yang Yang, Yang Li, Shaobo Zhou

“…Our results showed that γ-tocotrienol (2–8 μmol/L) significantly improved the cell proliferation (p<0.05), but it did not affect cell cycle progression. γ-Tocotrienol significantly increased alkaline phosphatase (ALP) activity (p<0.05), secretion levels of osteocalcin (OC) and osteonectin (ON), and mRNA levels of collagen type I (Col I) of MC3T3-E1 cells. …”
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The molecular prevalence of Theileria equi in the Arabian stallion in Egypt with special reference to biochemical parameters by Noora F. Thabet, Omaima M. Kandil, Omnia M. Kandil, Khalid M. Fararh

“…Total bilirubin, direct bilirubin, indirect bilirubin, GGT, GOT (AST), urea, and creatinine were significantly increased in the serum of the infected group compared with the healthy group; total protein, albumin, and globulin were found to be decreased in the serum of the infected group when compared to the healthy group. The ALP and albumin: globulin (A: G) ratio changes weren&rsquo;t significant in this study. …”
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CADD based designing and biological evaluation of novel triazole based thiazolidinedione coumarin hybrids as antidiabetic agent by Anchal Sharma, Anmol Narang, Nitish Kumar, Rupali Rana, Megha, Pooja, Muskan Dhir, Harmandeep Kaur Gulati, Jyoti, Aanchal Khanna, Jatinder Vir Singh, Sukhraj Kaur, Preet Mohinder Singh Bedi

“…Furthermore, Compound I-1, like Acarbose, resulted in significant reductions in ALT, AST, ALP, urea, LDH, and creatinine levels, suggesting improved liver and kidney function.…”
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Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling by Wei Qin, Qi-Ting Huang, Michael D. Weir, Zhi Song, Ashraf F. Fouad, Zheng-Mei Lin, Liang Zhao, Hockin H. K. Xu

“…In addition, EtOH downregulated the alkaline phosphatase activity, attenuated the mineralized nodule formation, and suppressed the expression of odontoblastic markers including ALP, DSPP, DMP-1, Runx2, and OCN. Moreover, the pretreatment with rapamycin, a specific mTOR inhibitor, markedly reversed the EtOH-induced odontoblastic differentiation and cell mineralization. …”
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Mitigative Effect of Erythromycin on PMMA Challenged Preosteoblastic MC3T3-E1 Cells by Yi Shen, Weili Wang, Xiaomiao Li, David C. Markel, Weiping Ren

“…Cell differentiation was determined by activity of alkaline phosphatase (ALP). Gene expression was measured via real-time quantitative RT-PCR. …”
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Treatment of Radiation Bone Injury with Transplanted hUCB-MSCs via Wnt/β-Catenin by Yufeng Zhang, Huaxin Deng, Zhiqiang Yang, Zhe Chen, Sheng Zhang, Yufan Zhu, Min Yang, Houcheng Zhong, Fuling Zhou, Yuanlong Xie, Lin Cai

“…The differentiation of osteoblasts was measured by ALP and Alizarin red staining and transmission electron microscopy. …”
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Effect of precise health management combined with physical rehabilitation on bone biomarkers in senile osteoporosis patients by Litao Chen, Xiaojing Yan, Yongjian Liu, Yongbin Pei, Jin Zhou, Lei Zhang, Zhixing Du

“…Additionally, scores on the Osteoporosis Knowledge Scale, Osteoporosis Self-Efficacy Scale, bone mineral density (BMD), ALP levels, and calcium levels were significantly higher in the observation group compared to the control group (P < 0.05). …”
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Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis by Xueqin Huang, Yingqi Yang, Hanlin Zhou, Liping Hu, Annan Yang, Hua Jin, Biying Zheng, Jiang Pi, Jun Xu, Pinghua Sun, Huai-Hong Cai, Xujing Liang, Bin Pan, Junxia Zheng, Haibo Zhou

“…In the presence of S. aureus, the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads (MBs) to release alkaline phosphatase (ALP), which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away. …”
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Biomedical application of gelatin-PVA-silk fibre composite reinforced with copper and manganese substituted hydroxyapatite by Sabareeswari Kalidas, Shanmugam Sumathi

“…The cell-viability study showed that MG-63 cells had successfully attached and grown on the scaffold surface. From the ALP activity it was found that osteoblast mineralization on the scaffolds increased as increasing the time interval. …”
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ET-1 Promotes Differentiation of Periodontal Ligament Stem Cells into Osteoblasts through ETR, MAPK, and Wnt/β-Catenin Signaling Pathways under Inflammatory Microenvironment by Li Liang, Wei Zhou, Nan Yang, Jifeng Yu, Hongchen Liu

“…The osteogenic potential of PDLSCs was tested using ALP staining. TNF-α, IL-1β, and IL-6 levels were evaluated by ELISA and western blot. …”
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Competitive and collaborative relationship between Al–3P and TiB2 nanoparticles in the microstructure manipulation of eutectic Al–Si alloys by Yi-Lu Li, Bai-Xin Dong, Hong-Yu Yang, Dan Luo, Shi-Li Shu, Zhi-Fa Wang, Jie Kang, Jia Meng, Chang-Jie Luo, Cheng-Gang Wang, Kuang Cao, Jian Qiao, Ming Zhu, Feng Qiu, Qi-Chuan Jiang

“…In this work, the synergistic effects of the modifier Al–3P master alloy and nano-TiB2 ceramic particles on the microstructure and mechanical performance of cast eutectic Al–Si alloys were investigated. Results showed that AlP and nano-TiB2 particles served as efficient heterogeneous nucleation sites of Si and α-Al, respectively. …”
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The effect of local propolis irrigation as an adjunct to scaling and root planning on alveolar bone loss in experimental periodontitis in rats (A biochemical and histological stu... by Khadeeja Mohammed Ali, Zewar Ahmad Saleh, Jalal Ali Jalal

“…Results: Rats that received local treatment by SRP with propolis irrigation in gp3 revealed features of acceleration of the periodontal tissue-repair process, alveolar bone formation, and significantly higher alkaline phosphatase serum levels (ALP) than gp2 were treated by SRP with vehicle irrigation. …”
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MEK1/2 Inhibitor (GDC0623) Promotes Osteogenic Differentiation of Primary Osteoblasts Inhibited by IL-1β through the MEK-Erk1/2 and Jak/Stat3 Pathways by Zeng-Qiao Zhang, Xiao-Shen Hu, Ye-Chen Lu, Jun-Peng Zhang, Wen-Yao Li, Wei-Yang Zhang, Wei Feng, Dao-Fang Ding, Jian-Guang Xu

“…Osteoblasts were cultured in an osteogenic induction medium for 1–3 weeks after which their differentiations were assessed by alkaline phosphatase (ALP) staining, Alizarin Red staining, calcium concentration, immunocytochemistry staining, real-time quantitative PCR (RT-qPCR), and immunofluorescence staining. …”
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Histological, physiological and transcriptomic analysis in hepatopancreas of Procambarus clarkii under heat stress by Yongfeng Zou, Panhui Cao, Zhiming Bao, Yu Xu, Zhiqiang Xu, Hui Guo

“…Significant fluctuations were observed in the malondialdehyde (MDA) content, reactive oxygen species (ROS) production, total antioxidant capacity (T-AOC), and activities of pyruvate kinase (PK), hexokinase (HK), alkaline phosphatase (ALP), lysozyme (LYS), acid phosphatase (ACP), fatty acid synthase (FAS), as well as lipoprotein lipase (LPL) in response to different stress conditions (P < 0.05). …”
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Semaphorin3B Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in a High-Glucose Microenvironment by Quan Xing, Jingyi Feng, Xiaolei Zhang

“…Furthermore, HG inhibited osteogenic differentiation in BMSCs by decreasing the expression of bone formation markers, alkaline phosphatase (ALP) activity, and mineralization. However, the administration of recombinant Sema3B reversed all of these effects. …”
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Perfluorooctane sulfonate (PFOS) induced bone loss by inhibiting FoxO1-mediated defense against oxidative stress in osteoblast by Liming Xue, Jiale Xu, Ping Xiao, Yiping Jiang, Yuanjie Lin, Chao Feng, Yu’e Jin, Zhijun Zhou, Guoquan Wang, Dasheng Lu

“…Our research is aimed at examining the effects of PFOS on osteoblastic activity and investigating the toxicological mechanisms of PFOS-induced bone loss. Cell proliferation, ALP activity, bone nodule formation, ROS levels, and cell apoptosis were assessed after treating osteoblasts with different concentrations of PFOS. …”
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Slowly Delivered Icariin/Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells to Promote the Healing of Calvarial Critical-Size Bone Defects by Tianlin Liu, Xin Zhang, Yuan Luo, Yuanliang Huang, Gang Wu

“…In in vitro cellular experiments, icariin at optimal concentration (10−6 mol/L) could significantly upregulate the osteogenesis- and angiogenesis-related genes and proteins, such as Runx2, ALP, osteocalcin, vascular endothelial growth factors, and fibroblast growth factors, as well as the mineralization of BMSCs. …”
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Automatic approach-avoidance tendency toward physical activity, sedentary, and neutral stimuli as a function of age, explicit affective attitude, and intention to be active by Farajzadeh, Ata, Goubran, Miriam, Beehler, Alexa, Cherkaoui, Noura, Morrison, Paula, de Chanaleilles, Margaux, Maltagliati, Silvio, Cheval, Boris, Miller, Matthew W., Sheehy, Lisa, Bilodeau, Martin, Orsholits, Dan, Boisgontier, Matthieu P.

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Pancreatic Metastasis of High-Grade Papillary Serous Ovarian Carcinoma Mimicking Primary Pancreas Cancer: A Case Report by Yusuf Gunay, Ebru Demiralay, Alp Demirag

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