EHMT2‐mediated R‐loop formation promotes the malignant progression of prostate cancer via activating Aurora B by Yuyang Zhang, Mingqin Su, Yiming Chen, Li Cui, Wei Xia, Renfang Xu, Dong Xue, Xiansheng Zhang, Xingliang Feng

“…Consequently, understanding how tumours adapt to CIN is critical for identifying novel therapeutic targets. Methods Bioinformatic analyses were conducted to identify genes overexpressed in PCa tissues using The Cancer Genome Atlas (TCGA) and GEO datasets. …”
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Design, synthesis, structural characterization, in vitro anticancer activity, and in silico studies of some new hydroxylated and fluorinated-substituted hydrazone derivatives by Reşit Çakmak, Eyüp Başaran, Ömer Erdoğan, Suraj N. Mali, Semiha Köprü, Haya Yasin, Shailesh S. Gurav, Giray Topal

“…Today, due to improved lifestyles and increased survival, the number of new cancer cases and cancer-related deaths continues to increase. In this study, novel hydroxylated and fluorinated-substituted hydrazone derivatives bearing an aromatic nitro moiety (2a-d and 3a-d) were designed as potential anticancer drug candidates, synthesized for the first time, and evaluated for their anticancer activity against chondrosarcoma (SW1353), a common primary malignant cartilage-forming tumor, neuroblastoma (SH-SY5Y), a type of brain cancer, and healthy (L929) cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method for 24 h. …”
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Genetic Analysis and Follow-Up of 25 Neonatal Diabetes Mellitus Patients in China by Bingyan Cao, Chunxiu Gong, Di Wu, Chaoxia Lu, Fang Liu, Xiaojing Liu, Yingxian Zhang, Yi Gu, Zhan Qi, Xiaoqiao Li, Min Liu, Wenjing Li, Chang Su, Xuejun Liang, Mei Feng

“…There were six non-KATP mutations, five novel mutations, including INS, EIF2AK3 (n=2), GLIS3, and SLC19A2, one known EIF2AK3 mutation. …”
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Electrocardiography-based artificial intelligence predicts the upcoming future of heart failure with mildly reduced ejection fraction by Dae-Young Kim, Sang-Won Lee, Dong-Ho Lee, Sang-Chul Lee, Sang-Chul Lee, Ji-Hun Jang, Sung-Hee Shin, Dae-Hyeok Kim, Dae-Hyeok Kim, Wonik Choi, Wonik Choi, Yong-Soo Baek, Yong-Soo Baek, Yong-Soo Baek

“…The AI-ECG encompasses a novel model that combines an automatic labelling preprocessing method with a transformer architecture incorporating a triplet loss for HFmrEF analysis.ResultsThe receiver operating characteristic analyses revealed that the area under the curve of AI-ECG for identifying all types of HF was acceptable [0.873, 95% confidence interval (CI): 0.864–0.893], while that for identifying patients with HFmrEF was relatively lower (0.824, 95% CI: 0.794–0.863) than that for those with HF with reduced ejection fraction (EF) (0.875, 95% CI: 0.844–0.912) and those with normal EF (0.870, 95% CI: 0.842–0.894). …”
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Cytotoxicity Potential of Endophytic Fungi Extracts from Terminalia catappa against Human Cervical Cancer Cells by Michèle Stella Majoumouo, Marius Belmondo Tincho, Rufin Marie Kouipou Toghueo, Thureyah Morris, Donavon Charles Hiss, Fabrice Fekam Boyom, Chitra Mandal

“…Endophytic fungi are potential sources of novel bioactive metabolites from a natural product drug discovery perspective. …”
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circASAP1 induces renal clear cell carcinoma ferroptosis by binding to HNRNPC and thereby regulating GPX4 by Yunfei Wang, Taowei Yang, Qihao Li, Zhousan Zheng, Lican Liao, Junjie Cen, Wei Chen, Junhang Luo, Yi Xu, Mi Zhou, Jiaxing Zhang

“…The circASAP1/HNRNPC/GPX4 axis provides novel directions and potential targets for RCC treatment.…”
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Integrating bulk and single-cell RNA sequencing data: unveiling RNA methylation and autophagy-related signatures in chronic obstructive pulmonary disease patients by Shi-Xia Liao, Lan-Ying Zhang, Ling-Mei Shi, Huai-Yu Hu, Yan-Hui Gu, Ting-Hua Wang, Yao Ouyang, Peng-Peng Sun

“…These findings provide novel genetic insights into the epigenetic mechanisms of COPD and suggest potential avenues for developing diagnostic and therapeutic strategies.…”
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Development of a horizontal transmission model to assess the effects of exposure to pathogenic E. cecorum during the hatching phase in broiler chickens by James Higuita, Marcela Arango, Aaron Forga, Mitch Rowland, Jundi Liu, Ross Wolfenden, Danielle Graham

“…These results suggest that horizontal transmission of EC in commercial hatch cabinets could be possible, and that persistent EC colonization and organ involvement may affect performance and disease progression in young broiler chickens depending on the EC strain. Novel strategies to control EC infections pre- and post-hatch could be evaluated using the model developed herein.…”
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Recovery trajectories of mental health symptoms among Chinese adolescents following the end of COVID-19 by Jun Peng, Meihui He, Yongxing Guo, Yongxing Guo, Yongxing Guo, Jiangdong Diao, Jiangdong Diao, Kun Chen, Ziyi Deng, Lei Mo, Ruixiang Gao

“…This study aims to examine the recovery trajectories of ten mental health problems and suicidal ideation among Chinese adolescents post-pandemic, with a focus on identifying factors that influence different recovery patterns.MethodsA total of 2,534 adolescents participated in a three-wave survey, conducted in June 2022, November 2022, and March 2023, using the Mental Health Scale for Chinese Middle School Students. A novel index was developed to account for both the number and severity of mental health risk factors, allowing for the classification of psychological symptoms into three subgroups: no, moderate, and severe. …”
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Post‐Polymerization Strategy via Dual Site Clicking for Synthesizing Intrinsically Cross‐Linkable Semiconducting Polymers by Jaehoon Lee, Seungju Kang, Eunsoo Lee, Jiyun Lee, Tae Woong Yoon, Min‐Jae Kim, Yongjoon Cho, Mingfei Xiao, Yorrick Boeije, Wenjin Zhu, Changduk Yang, Jin‐Wook Lee, Sungjoo Lee, Guobing Zhang, Henning Sirringhaus, Boseok Kang

“…In this study, this limitation is overcome by a novel post‐polymerization strategy. A reagent with a cross‐linkable functional group, (3‐mercaptopropyl)trimethoxysilane, is attached to a diketopyrrolopyrrole‐based donor–acceptor copolymer (DPPTT) via thioesterification and para‐fluoro‐thiol reaction, modifying two sites simultaneously. …”
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CaCO3-coated indoxacarb deep eutectic solvent complexed with diatomaceous earth improves insecticidal activity against the red imported fire ants by Jiantao Fu, Junfang Wang, Zewen Ma, Di Yuan, Yunfei Zhang, Lanying Wang, Yanping Luo

“…Therefore, our research provides a simple and feasible approach for designing and constructing novel nanopesticides for RIFAs control.…”
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Feasibility of an indocyanine green-hyaluronic acid mixture (LuminoMark™) for targeting suspicious axillary lymph nodes in patients with breast cancer by Jeeyeon Lee, Byeongju Kang, Jin Hyang Jung, Hye Jung Kim, Won Hwa Kim, Jung Dug Yang, Joon Seok Lee, Yee Soo Chae, Soo Jung Lee, In Hee Lee, Ji-Young Park, Nora Jee-Young Park, Ho Yong Park

“…Abstract Purpose During breast cancer surgery, the use of dyes such as indigo carmine, methylene blue, or indocyanine green (ICG) for targeting axillary lymph nodes (ALNs) under ultrasound guidance can result in rapid diffusion, complicated tissue differentiation, and disruption of staining. LuminoMark™, a novel ICG-hyaluronic acid mixture, can provide real-time visualization and minimize dye spread, thereby ensuring a clear surgical field. …”
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Characterization of the genomic landscape of canine diffuse large B-cell lymphoma reveals recurrent H3K27M mutations linked to progression-free survival by Anna Darlene van der Heiden, Raphaela Pensch, Sophie Agger, Heather L. Gardner, William Hendricks, Victoria Zismann, Shukmei Wong, Natalia Briones, Bryce Turner, Karin Forsberg-Nilsson, Cheryl London, Kerstin Lindblad-Toh, Maja Louise Arendt

“…These findings underscore the critical role of epigenetic dysregulation in cDLBCL and affirm the dog as a relevant large animal model for interrogating the biological activity of novel histone-modifying treatment strategies.…”
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Genetic and peripheral biomarkers of comorbid posttraumatic stress disorder and traumatic brain injury: a systematic review by Kiriana Cowansage, Reshmi Nair, Jose M. Lara-Ruiz, Daniel E. Berman, Courtney C. Boyd, Tiffany L. Milligan, Daniel Kotzab, Dawn M. Bellanti, Lisa M. Shank, Maria A. Morgan, Derek J. Smolenski, Ida Babakhanyan, Nancy A. Skopp, Daniel P. Evatt, Marija S. Kelber

“…Additional research is needed to determine how such factors indicate, predict, and contribute to comorbidity and to what extent they represent viable targets for the development of novel diagnostic tools and therapeutic interventions.…”
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Effect of cisplatin/gold chitosan nanocomposite on oral squamous cell carcinoma and oral epithelial cells by Basma Abdelrahman Ahmed, Rania Osama M. Mohsen, Marwa Sharaky, Marwa A. Ramadan, Amna H. Faid, Mai Hafez Mohamed

“…Thus, we synthesized a novel agent of cisplatin coupled to gold chitosan nanoparticles (Cis/AuCh nanocomposite) and examined its effect in addition to the effect of chitosan-reduced gold nanoparticles (AuCh Nps) on (HNO97) OSCC cell line and normal oral epithelial cells (OEC). …”
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Cardiac manifestations of Fabry disease in G3Stg/GlaKO and GlaKO mouse models-Translation to Fabry disease patients. by Abirami Kugadas, Pietro Artoni, Wanida Ruangsiriluk, Meng Zhao, Natalia Boukharov, Rizwana Islam, Dmitri Volfson, Katayoun Derakhchan

“…In conclusion, GlaKO mouse model presents mild cardiomegaly, mild cardiac dysfunction, but significant cardiac volume overload and functional changes in GLS that can be used as translational biomarkers to determine cardiac efficacy of novel treatment modalities. The level of tissue Gb3 accumulation in G3Stg/GlaKO mouse more closely recapitulates the level of substrate accumulation in FD patients and may provide better translatability of the efficacy of new therapeutics in clearing pathological substrates from cardiac tissues. …”
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Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology by Ji X, Li Y, Wang J, Wang G, Ma B, Shi J, Cui C, Wang R

“…Given the limited production and restricted properties of natural silk proteins, molecular biotechnology has been extensively applied in silk protein genetic engineering to produce novel silk proteins with specific properties. This review outlines the roles of major model organisms, such as silkworms and spiders, in silk protein production, and provides a detailed introduction to the applications of gene editing technologies (eg, CRISPR-Cas9), transgenic expression technologies, and synthetic biology techniques in silk protein genetic engineering. …”
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Deep Learning‐Assisted Label‐Free Parallel Cell Sorting with Digital Microfluidics by Zongliang Guo, Fenggang Li, Hang Li, Menglei Zhao, Haobing Liu, Haopu Wang, Hanqi Hu, Rongxin Fu, Yao Lu, Siyi Hu, Huikai Xie, Hanbin Ma, Shuailong Zhang

“…Abstract Sorting specific cells from heterogeneous samples is important for research and clinical applications. In this work, a novel label‐free cell sorting method is presented that integrates deep learning image recognition with microfluidic manipulation to differentiate cells based on morphology. …”
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Large-scale evidence generation and evaluation across a network of databases for type 2 diabetes mellitus (LEGEND-T2DM): a protocol for a series of multinational, real-world compar... by Rohan Khera, Yuan Lu, Harlan M Krumholz, Martijn J Schuemie, Ruijun Chen, George Hripcsak, Marc A Suchard, Patrick B Ryan, Anna Ostropolets

“…Introduction Therapeutic options for type 2 diabetes mellitus (T2DM) have expanded over the last decade with the emergence of cardioprotective novel agents, but without such data for older drugs, leaving a critical gap in our understanding of the relative effects of T2DM agents on cardiovascular risk.Methods and analysis The large-scale evidence generations across a network of databases for T2DM (LEGEND-T2DM) initiative is a series of systematic, large-scale, multinational, real-world comparative cardiovascular effectiveness and safety studies of all four major second-line anti-hyperglycaemic agents, including sodium–glucose co-transporter-2 inhibitor, glucagon-like peptide-1 receptor agonist, dipeptidyl peptidase-4 inhibitor and sulfonylureas. …”
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The p110α and p110β Isoforms of Class I Phosphatidylinositol 3-Kinase Are Involved in Toll-Like Receptor 5 Signaling in Epithelial Cells by Sabine M. Ivison, Mohammed A. S. Khan, Nicholas R. Graham, Leila A. Shobab, Yu Yao, Arnawaz Kifayet, Laura M. Sly, Theodore S. Steiner

“…Using a plasmid-based shRNA delivery system and novel p110 isoform-specific inhibitors, we found that flagellin-induced IL-8 production was dependent on both p110α and p110β. …”
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