Allele-specific PCR with fluorescently labeled probes: criteria for selecting primers for genotyping by V. A. Devyatkin, A. A. Shklyar, A. Zh. Fursova, Yu. V. Rumyantseva, O. S. Kozhevnikova

“…Whole-genome sequencing and genotyping by sequencing can detect SNPs with high specificity and identify novel variants. Nonetheless, in situations where the interest of researchers is individual specific loci, these methods become redundant, and their cost, the proportion of false positive and false negative results, and labor costs for sample preparation and analysis do not justify their use. …”
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Normalization of Cystic Fibrosis Immune System Reverses Intestinal Neutrophilic Inflammation and Significantly Improves the Survival of Cystic Fibrosis MiceSummary by Callie E. Scull, Yawen Hu, Scott Jennings, Guoshun Wang

“…Thus, normalization of the CF immune system may offer a novel therapy to treat CF intestinal disease.…”
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Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells by Corinne Kai Leng Chew, Hannah O'Toole, Daniela M Zisterer, Seema M. Nathwani, Jade K. Pollock

“…However, many cancer cells are resistant to TRAIL monotherapy. A novel microtubule-targeting agent, PBOX-15 is known to exhibit anti-tumour activity in cancer cell lines including those with multi-drug resistance. …”
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Applying YOLOv6 as an ensemble federated learning framework to classify breast cancer pathology images by Chhaya Gupta, Nasib Singh Gill, Preeti Gulia, Noha Alduaiji, J. Shreyas, Piyush Kumar Shukla

“…A new homomorphic encryption and decryption algorithm is also proposed to retain data privacy. A novel pruned YOLOv6 model with FedL is introduced in this study to differentiate benign and malignant tissues. …”
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Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway by Yuting Zhang, Mingliang Jiang, Shurui Sun, Zongxiang Zhan, Xiaonan Li, Zhongyun Piao

“…This study provides new theoretical insights for addressing premature bolting in Chinese cabbage and offers novel approaches for breeding bolting-resistant varieties.…”
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Thermally Conductive Shape-Stabilized Phase Change Materials Enabled by Paraffin Wax and Nanoporous Structural Expanded Graphite by Yilin Zhao, Shuhui Huang, Zhaoguo Jin, Zhongnan Xie, Hong Guo, Haofeng Xie

“…In addition, due to the excellent thermal management capability of PW/EG, the operating temperature of electronic components can be stably maintained at 68–71 °C for about 15 min and the peak temperature can be reduced by at least 23 °C when the heating power of the electronic components is 10 w. These provide novel and cost-effective methods to further improve the management capability of spacecraft thermal control systems.…”
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Lunasin protease inhibitor concentrate decreases pro-inflammatory cytokines and improves histopathological markers in dextran sodium sulfate-induced ulcerative colitis by Andrea Nieto-Veloza, Zhihong Wang, Qixin Zhong, Doris D’Souza, Hari B. Krishnan, Vermont P. Dia

“…Lunasin protease inhibitor concentrate (LPIC) is a novel combination of soy bioactive peptide lunasin, Kunitz and Bowman-Birk protease inhibitors. …”
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Additively manufactured stochastic and gyroid scaffold design towards osseointegration and bone regeneration in a rabbit femur model by Susheem Kanwar, Oraib Al-Ketan, Gopinathan Janarthanan, Sanjairaj Vijayavenkataraman

“…This evolution brought along better response to implants in terms of compatibility and promotion of cell ingrowth. The use of novel designs like stochastic designs, impart the user with the ability to locally control the porosity of the scaffold and thus fine tune its functional and structural properties like stiffness and porosity gradient. …”
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Assessing Mitigation Strategies to Reduce Potential Exposures to Indoor Particle Release Events by Katie Van Valkinburgh, Ali Mohammadi Nafchi, Ehsan Mousavi, Vincent Blouin, Nigel Kaye, Andrew R. Metcalf

“…Abstract Airborne transmission is a major concern for many infectious pathogens, including the novel coronavirus. Ventilation is the principal engineering method used to control airborne health hazards in buildings. …”
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Distinguishing benign and malignant myxoid soft tissue tumors: Performance of radiomics vs. radiologists. by Joshua M Lawrenz, Can Cui, Samuel R Johnson, Katherine S Hajdu, Stephen W Chenard, Akhil Rekulapelli, Cullen P Moran, John J Block, Nicholson S Chadwick, Joanna L Shechtel, Brian Bingham, Leo Y Luo, Jennifer L Halpern, Herbert S Schwartz, Ginger E Holt, David S Smith, Benoit Dawant, Hakmook Kang

“…Though radiomics and radiologists perform similarly overall, radiomics may provide novel diagnostic utility when radiologist confidence is low, or when radiologists disagree.…”
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Skin Lesion Classification Through Test Time Augmentation and Explainable Artificial Intelligence by Loris Cino, Cosimo Distante, Alessandro Martella, Pier Luigi Mazzeo

“…The primary objective of this study is to develop a highly accurate AI-based algorithm for skin lesion classification that also provides visual explanations to foster trust and confidence in these novel diagnostic tools. By improving transparency, the study seeks to contribute to earlier and more reliable diagnoses. …”
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The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 in c-kit Detected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells by A. Sailasuta, D. Ketpun, P. Piyaviriyakul, S. Theerawatanasirikul, P. Theewasutrakul, A. Rungsipipat

“…In this study, our biopsy specimens were classified as low- and high-grade MCT based on the novel 2-tier histopathologic grading system. We have demonstrated the usage of fine-needle aspirated MCT cells (FNA-MCT cells) from these specimens as a primary cell source to study the distribution of CD117-immunocytochemistry (CD117-ICC) staining patterns and the frequency of internal tandem duplication- (ITD-) mutant exon-11 of c-kit. …”
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Explore Alteration of Lung and Gut Microbiota in a Murine Model of OVA-Induced Asthma Treated by CpG Oligodeoxynucleotides by Wang Q, Ji J, Xiao S, Wang J, Yan X, Fang L

“…After CpG-ODN administration, bacterial dysbiosis was improved, and a notable enrichment of beneficial bacteria, indicating a novel microecology. Meanwhile Oscillospira and Clostridium were identified as two biomarkers of the CpG-ODN treatment. (3) Heatmap analysis revealed significant correlations among lung, small intestine, and colon microbiota.Conclusion: CpG-ODN treatment can ameliorate OVA-induced asthma in mice. …”
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Fault diagnosis of a CNC hobbing cutter through machine learning using three axis vibration data by Nagesh Tambake, Bhagyesh Deshmukh, Sujit Pardeshi, Sachin Salunkhe, Robert Cep, Emad Abouel Nasr

“…This research presents a novel approach to fault diagnosis for CNC hobbing cutters using machine learning techniques, leveraging three-axis vibration data to ensure machining precision and tool reliability. …”
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Securing fog computing in healthcare with a zero-trust approach and blockchain by Navjeet Kaur, Ayush Mittal, Umesh Kumar Lilhore, Sarita Simaiya, Surjeet Dalal, Kashif Saleem, Ehab Seif Ghith

“…Therefore, this paper proposes a novel security framework that integrates blockchain (BC) and software-defined network (SDN) technologies, underpinned by zero-trust (ZT) principles, to address these challenges in latency-sensitive healthcare environments. …”
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Cumulative Energy Demand Analysis of Commercial and Hybrid Metal-Composite Gears at Different End-of-Life Strategies by Francesco Borda, Rocco Adduci, Domenico Mundo, Francesco Gagliardi

“…Traditionally, material removal from gear bodies results in weight reduction, but at the cost of increased noise and vibration. A novel approach using hybrid gears, which combine a metal rim and hub with a composite material web, offers a promising solution. …”
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Microglia Play a Major Role in Direct Viral-Induced Demyelination by Dhriti Chatterjee, Kaushiki Biswas, Soma Nag, S. G. Ramachandra, Jayasri Das Sarma

“…Current studies revealed that MHV infection is associated with the pronounced activation of microglia during acute inflammation, as evidenced by characteristic changes in cellular morphology and increased expression of microglia-specific proteins, Iba1 (ionized calcium-binding adaptor molecule 1), which is a macrophage/microglia-specific novel calcium-binding protein and involved in membrane ruffling and phagocytosis. …”
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Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom by Daniel Moreira dos Santos, Livia Ramos Santiago, Nayara Araújo dos Santos, Wanderson Romão, Jarbas Magalhães Resende, Maria Elena de Lima, Márcia Helena Borges, Rosy Iara Maciel de Azambuja Ribeiro

“…The discovery of novel cytotoxic drugs is of paramount importance in contemporary medical research, particularly in the search for treatments with fewer side effects and higher specificity. …”
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Using β-Elemene to reduce stemness and drug resistance in osteosarcoma: A focus on the AKT/FOXO1 signaling pathway and immune modulation by Shaochun Zhang, Zhijie Xing, Jing Ke

“…These findings suggest β-elemene as a potential adjunct therapy for osteosarcoma, providing novel therapeutic strategies to overcome chemotherapy resistance and improve patient outcomes.…”
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Significance of siderophore-producing cyanobacteria on enhancing iron uptake potentiality of maize plants grown under iron-deficiency by Mandees Bakr Brick, Mervat H. Hussein, Amr M. Mowafy, Ragaa A. Hamouda, Amr M. Ayyad, Dina A. Refaay

“…Findings of this study present novel visions of cyanobacteria producing siderophores as an ecofriendly alternative candidate to synthetic iron chelators and their role in plant stress management.…”
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