Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart Disease
Red wine grape pomace (RWGP), a winemaking by-product rich in phenolics, flavonoids, and dietary fiber, has shown promise in mitigating cardiovascular disease (CVD), however, its mechanisms of action remain incompletely understood. This study comprehensively profiled the phenolic composition of RWGP...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/14/5/574 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850127509865103360 |
|---|---|
| author | Katherine Rivera Leticia González Laura Parra Juan E. Oyarzún Alina Concepción-Alvarez Adriano Costa de Camargo Raquel Bridi Attilio Rigotti Marcelo E. Andia |
| author_facet | Katherine Rivera Leticia González Laura Parra Juan E. Oyarzún Alina Concepción-Alvarez Adriano Costa de Camargo Raquel Bridi Attilio Rigotti Marcelo E. Andia |
| author_sort | Katherine Rivera |
| collection | DOAJ |
| description | Red wine grape pomace (RWGP), a winemaking by-product rich in phenolics, flavonoids, and dietary fiber, has shown promise in mitigating cardiovascular disease (CVD), however, its mechanisms of action remain incompletely understood. This study comprehensively profiled the phenolic composition of RWGP—including free, esterified, etherified, and insoluble-bound fractions—and evaluated the effects of RWGP dietary supplementation on gut barrier integrity, inflammation, oxidative stress, and survival in SR-B1<sup>−/−</sup>ApoE-R61<sup>h/h</sup> mice, a model of diet-induced lethal ischemic heart disease. RWGP supplementation significantly improved survival rates and restored gut barrier function, as evidenced by lower plasma FITC-dextran and LPS levels, increased circulating ZO-1 levels, and reduced histopathological colon damage. In addition, RWGP reduced pro-inflammatory cytokines (IL-1<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>β</mi></semantics></math></inline-formula>) and showed a trend toward attenuating systemic oxidative stress (TBARS). Analysis of phenolic compounds indicated a significant presence of insoluble-bound phenolics. Nevertheless, the beneficial effects observed are likely attributable to the synergistic actions of RWGP’s complex phytochemical and fiber composition. These results highlight RWGP’s potential as a sustainable, gut-targeted functional food ingredient for CVD prevention and management. |
| format | Article |
| id | doaj-art-ffaab53a8bfb4beaa5244e714e1c4a4e |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-ffaab53a8bfb4beaa5244e714e1c4a4e2025-08-20T02:33:39ZengMDPI AGAntioxidants2076-39212025-05-0114557410.3390/antiox14050574Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart DiseaseKatherine Rivera0Leticia González1Laura Parra2Juan E. Oyarzún3Alina Concepción-Alvarez4Adriano Costa de Camargo5Raquel Bridi6Attilio Rigotti7Marcelo E. Andia8Doctoral Program in Medical Sciences, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago 8331010, ChileRadiology Department and Biomedical Imaging Center, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileDoctoral Program in Medical Sciences, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago 8331010, ChileRadiology Department and Biomedical Imaging Center, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileNutrition and Food Technology Institute, University of Chile, Santiago 7830490, ChileNutrition and Food Technology Institute, University of Chile, Santiago 7830490, ChileDepartamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380494, ChileCentro de Nutrición Molecular y Enfermedades Crónicas, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago 8331010, ChileRadiology Department and Biomedical Imaging Center, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 7820436, ChileRed wine grape pomace (RWGP), a winemaking by-product rich in phenolics, flavonoids, and dietary fiber, has shown promise in mitigating cardiovascular disease (CVD), however, its mechanisms of action remain incompletely understood. This study comprehensively profiled the phenolic composition of RWGP—including free, esterified, etherified, and insoluble-bound fractions—and evaluated the effects of RWGP dietary supplementation on gut barrier integrity, inflammation, oxidative stress, and survival in SR-B1<sup>−/−</sup>ApoE-R61<sup>h/h</sup> mice, a model of diet-induced lethal ischemic heart disease. RWGP supplementation significantly improved survival rates and restored gut barrier function, as evidenced by lower plasma FITC-dextran and LPS levels, increased circulating ZO-1 levels, and reduced histopathological colon damage. In addition, RWGP reduced pro-inflammatory cytokines (IL-1<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>β</mi></semantics></math></inline-formula>) and showed a trend toward attenuating systemic oxidative stress (TBARS). Analysis of phenolic compounds indicated a significant presence of insoluble-bound phenolics. Nevertheless, the beneficial effects observed are likely attributable to the synergistic actions of RWGP’s complex phytochemical and fiber composition. These results highlight RWGP’s potential as a sustainable, gut-targeted functional food ingredient for CVD prevention and management.https://www.mdpi.com/2076-3921/14/5/574phenolic compoundsred wine grape pomaceendotoxemiagut permeabilityischemic heart diseasegut–heart axis |
| spellingShingle | Katherine Rivera Leticia González Laura Parra Juan E. Oyarzún Alina Concepción-Alvarez Adriano Costa de Camargo Raquel Bridi Attilio Rigotti Marcelo E. Andia Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart Disease Antioxidants phenolic compounds red wine grape pomace endotoxemia gut permeability ischemic heart disease gut–heart axis |
| title | Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart Disease |
| title_full | Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart Disease |
| title_fullStr | Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart Disease |
| title_full_unstemmed | Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart Disease |
| title_short | Red Wine Grape Pomace Restores Gut Barrier Function and Improves Survival in Diet-Induced Ischemic Heart Disease |
| title_sort | red wine grape pomace restores gut barrier function and improves survival in diet induced ischemic heart disease |
| topic | phenolic compounds red wine grape pomace endotoxemia gut permeability ischemic heart disease gut–heart axis |
| url | https://www.mdpi.com/2076-3921/14/5/574 |
| work_keys_str_mv | AT katherinerivera redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease AT leticiagonzalez redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease AT lauraparra redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease AT juaneoyarzun redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease AT alinaconcepcionalvarez redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease AT adrianocostadecamargo redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease AT raquelbridi redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease AT attiliorigotti redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease AT marceloeandia redwinegrapepomacerestoresgutbarrierfunctionandimprovessurvivalindietinducedischemicheartdisease |