Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans
Abstract The Balkan Peninsula hosts a great proportion of Europe’s biodiversity, and this is well illustrated by amphibian richness and endemism. Among them, the yellow-bellied toad Bombina variegata has been a model in ecology and evolution, but several aspects of its phylogeography and ta...
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| Format: | Article |
| Language: | English |
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Pensoft
2025-01-01
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| Series: | Vertebrate Zoology |
| Online Access: | https://vertebrate-zoology.arphahub.com/article/138687/download/pdf/ |
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| author | Christophe Dufresnes Simeon Lukanov Sven Gippner Johanna Ambu Ilias Strachinis Dragan Arsovski Benjamin Monod-Broca Hugo Cayuela Petros Lymberakis Daniele Canestrelli Dan Cogălniceanu Nikolay A. Poyarkov Spartak N. Litvinchuk Tomasz Suchan Mathieu Denoël Daniel Jablonski |
| author_facet | Christophe Dufresnes Simeon Lukanov Sven Gippner Johanna Ambu Ilias Strachinis Dragan Arsovski Benjamin Monod-Broca Hugo Cayuela Petros Lymberakis Daniele Canestrelli Dan Cogălniceanu Nikolay A. Poyarkov Spartak N. Litvinchuk Tomasz Suchan Mathieu Denoël Daniel Jablonski |
| author_sort | Christophe Dufresnes |
| collection | DOAJ |
| description | Abstract The Balkan Peninsula hosts a great proportion of Europe’s biodiversity, and this is well illustrated by amphibian richness and endemism. Among them, the yellow-bellied toad Bombina variegata has been a model in ecology and evolution, but several aspects of its phylogeography and taxonomy remain surprisingly poorly understood. In this study, we combine cytochrome b DNA barcoding data (1238 individuals from 355 localities), mitogenome phylogenetics (17.2 kb), gene-based nuclear phylogenetics (3.7 kb from four gene fragments) and multilocus phylogenomics (4759 loci / ~554 kb obtained by double digest Restriction Associated DNA sequencing; ddRAD-seq) to re-assess the diversification of B. variegata, and revisit its nomenclatural history to assign scientific names to phylogeographic lineages. The analyses support four major lineages, one assigned to B. v. variegata (Carpathians and northwestern ranges), one assigned to B. v. pachypus (Apennine Peninsula), and two assigned to B. v. scabra (Dinarides, Hellenides and Balkanides vs. the Rhodope mountains). Spatiotemporal patterns of diversification suggest a role for a Late Miocene marine incursion in the Pannonian Plain (Paratethys) as the initial trigger of divergence, followed by a vicariance event in the Apennines and a “sky island” process of Pleistocene differentiation in the Balkan Peninsula. As it reached the Dinarides during the Late Pleistocene, B. v. variegata potentially hybridized with B. v. scabra and captured its mitochondrial DNA, which resulted in a massive cyto-nuclear discordance across all northwestern European populations. Finally, we show that the two lineages of B. v. scabra significantly differ in morphology and ventral coloration patterns, and describe the Rhodope lineage as a new subspecies. |
| format | Article |
| id | doaj-art-ff9f722b1bee45bbaa9deb267097b158 |
| institution | Kabale University |
| issn | 2625-8498 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Pensoft |
| record_format | Article |
| series | Vertebrate Zoology |
| spelling | doaj-art-ff9f722b1bee45bbaa9deb267097b1582025-01-25T08:31:59ZengPensoftVertebrate Zoology2625-84982025-01-017513010.3897/vz.75.e138687138687Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the BalkansChristophe Dufresnes0Simeon Lukanov1Sven Gippner2Johanna Ambu3Ilias Strachinis4Dragan Arsovski5Benjamin Monod-Broca6Hugo Cayuela7Petros Lymberakis8Daniele Canestrelli9Dan Cogălniceanu10Nikolay A. Poyarkov11Spartak N. Litvinchuk12Tomasz Suchan13Mathieu Denoël14Daniel Jablonski15Sorbonne UniversitéInstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of SciencesTechnical University of BraunschweigNanjing Forestry UniversityAristotle University of ThessalonikiMacedonian Ecological SocietyUniversité Claude Bernard Lyon 1Université Claude Bernard Lyon 1University of CreteUniversity of TusciaUniversity Ovidius ConstanţaLomonosov Moscow State UniversityInstitute of Cytology, Russian Academy of SciencesW. Szafer Institute of Botany, Polish Academy of SciencesUniversity of LiègeComenius University in BratislavaAbstract The Balkan Peninsula hosts a great proportion of Europe’s biodiversity, and this is well illustrated by amphibian richness and endemism. Among them, the yellow-bellied toad Bombina variegata has been a model in ecology and evolution, but several aspects of its phylogeography and taxonomy remain surprisingly poorly understood. In this study, we combine cytochrome b DNA barcoding data (1238 individuals from 355 localities), mitogenome phylogenetics (17.2 kb), gene-based nuclear phylogenetics (3.7 kb from four gene fragments) and multilocus phylogenomics (4759 loci / ~554 kb obtained by double digest Restriction Associated DNA sequencing; ddRAD-seq) to re-assess the diversification of B. variegata, and revisit its nomenclatural history to assign scientific names to phylogeographic lineages. The analyses support four major lineages, one assigned to B. v. variegata (Carpathians and northwestern ranges), one assigned to B. v. pachypus (Apennine Peninsula), and two assigned to B. v. scabra (Dinarides, Hellenides and Balkanides vs. the Rhodope mountains). Spatiotemporal patterns of diversification suggest a role for a Late Miocene marine incursion in the Pannonian Plain (Paratethys) as the initial trigger of divergence, followed by a vicariance event in the Apennines and a “sky island” process of Pleistocene differentiation in the Balkan Peninsula. As it reached the Dinarides during the Late Pleistocene, B. v. variegata potentially hybridized with B. v. scabra and captured its mitochondrial DNA, which resulted in a massive cyto-nuclear discordance across all northwestern European populations. Finally, we show that the two lineages of B. v. scabra significantly differ in morphology and ventral coloration patterns, and describe the Rhodope lineage as a new subspecies.https://vertebrate-zoology.arphahub.com/article/138687/download/pdf/ |
| spellingShingle | Christophe Dufresnes Simeon Lukanov Sven Gippner Johanna Ambu Ilias Strachinis Dragan Arsovski Benjamin Monod-Broca Hugo Cayuela Petros Lymberakis Daniele Canestrelli Dan Cogălniceanu Nikolay A. Poyarkov Spartak N. Litvinchuk Tomasz Suchan Mathieu Denoël Daniel Jablonski Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans Vertebrate Zoology |
| title | Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans |
| title_full | Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans |
| title_fullStr | Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans |
| title_full_unstemmed | Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans |
| title_short | Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans |
| title_sort | historical biogeography and systematics of yellow bellied toads bombina variegata with the description of a new subspecies from the balkans |
| url | https://vertebrate-zoology.arphahub.com/article/138687/download/pdf/ |
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