Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome

Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome

Abstract Extracellular vesicles (EVs) in plasma are composed of exosomes, microvesicles, and apoptotic bodies. We report a plasma EV enrichment strategy using magnetic beads called Mag-Net. Proteomic interrogation of this plasma EV fraction enables the detection of proteins that are beyond the dynam...

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Main Authors: Christine C. Wu, Kristine A. Tsantilas, Jea Park, Deanna Plubell, Justin A. Sanders, Previn Naicker, Ireshyn Govender, Sindisiwe Buthelezi, Stoyan Stoychev, Justin Jordaan, Gennifer Merrihew, Eric Huang, Edward D. Parker, Michael Riffle, Andrew N. Hoofnagle, William S. Noble, Kathleen L. Poston, Thomas J. Montine, Michael J. MacCoss
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-60595-7
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https://doi.org/10.1038/s41467-025-60595-7

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