Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem Cells
Background: Root Canal Treatments play a critical role in managing periapical and pulp diseases, but failure to control the inflammation may result in treatment failure. Newer developments have targeted anti-inflammatory bioactive sealers as potential contenders. The objective of this study was to...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
ziauddin University
2025-01-01
|
| Series: | Pakistan Journal of Medicine and Dentistry |
| Subjects: | |
| Online Access: | https://ojs.zu.edu.pk/pjmd/article/view/3420 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594256195223552 |
|---|---|
| author | Ayesha Imtiaz Kiran Nayyar Khadija Akhtar Muhammad Yousuf Ali Naresh kumar Ehsan Ul Haq |
| author_facet | Ayesha Imtiaz Kiran Nayyar Khadija Akhtar Muhammad Yousuf Ali Naresh kumar Ehsan Ul Haq |
| author_sort | Ayesha Imtiaz |
| collection | DOAJ |
| description |
Background: Root Canal Treatments play a critical role in managing periapical and pulp diseases, but failure to control the inflammation may result in treatment failure. Newer developments have targeted anti-inflammatory bioactive sealers as potential contenders. The objective of this study was to explore the anti-inflammatory potential of Endoseal E-MTA (E-MTA), Ketac-Endo (Ketac-E), and Sealapex (S-apex) on cultured Human periodontal ligament stem cells.
Methods: After Study approval (BMU-EC/06-2022) from Baqai Medical University Karachi, this study was conducted from September 2022 to February 2023. Three Sealers as commercially available products were used on the cultured hPDLSCs via in-vitro experiments and treated with serial concentrations of 20ul, 40ul, and 60ul respectively to perform MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] cell viability assay. Expression profiling via Real Time-PCR of anti-inflammatory biometers was determined via RNA extract from 24h MTT assay due to its greater reproducibility. Data were subjected to statistical analysis (ANOVA Testing) to compare the effectiveness of each sealer using SPSS version 22 with a significant p-value<0.05.
Results: E-MTA elicits a noticeably enhanced production of IL-10 (2.97 fold) and TGF-β (3.12 fold) levels than its parent compound MTA, demonstrating enhanced anti-inflammatory and immunomodulatory potential while Ketac-Endo and Sealapex showed lesser relative gene fold values
Conclusion: It has been concluded that E-MTA exhibits more enhanced bioactivity and capacity to improve the RCT outcome due to reduced inflammation and increased tissue regeneration than Ketac-Endo and Sealapex.
|
| format | Article |
| id | doaj-art-ff7bc091babe409485e1de9e24068259 |
| institution | Kabale University |
| issn | 2313-7371 2308-2593 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | ziauddin University |
| record_format | Article |
| series | Pakistan Journal of Medicine and Dentistry |
| spelling | doaj-art-ff7bc091babe409485e1de9e240682592025-01-19T20:18:26Zengziauddin UniversityPakistan Journal of Medicine and Dentistry2313-73712308-25932025-01-0114110.36283/ziun-pjmd14-1/019Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem CellsAyesha Imtiaz0Kiran Nayyar1Khadija Akhtar2Muhammad Yousuf Ali3Naresh kumar4Ehsan Ul Haq5Baqai Medical University, Karachi, Pakistan.de Montmorency College of Dentistry, Lahore, PakistanInstitute Of Dentistry, CMH Lahore Medical College, PakistanKarachi Medical and Dental College (KMU), PakistanBhitai Dental and Medical College mirpurkhas sindh, Pakistan.School of Biochemistry, Free University of Berlin, Germany. Background: Root Canal Treatments play a critical role in managing periapical and pulp diseases, but failure to control the inflammation may result in treatment failure. Newer developments have targeted anti-inflammatory bioactive sealers as potential contenders. The objective of this study was to explore the anti-inflammatory potential of Endoseal E-MTA (E-MTA), Ketac-Endo (Ketac-E), and Sealapex (S-apex) on cultured Human periodontal ligament stem cells. Methods: After Study approval (BMU-EC/06-2022) from Baqai Medical University Karachi, this study was conducted from September 2022 to February 2023. Three Sealers as commercially available products were used on the cultured hPDLSCs via in-vitro experiments and treated with serial concentrations of 20ul, 40ul, and 60ul respectively to perform MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] cell viability assay. Expression profiling via Real Time-PCR of anti-inflammatory biometers was determined via RNA extract from 24h MTT assay due to its greater reproducibility. Data were subjected to statistical analysis (ANOVA Testing) to compare the effectiveness of each sealer using SPSS version 22 with a significant p-value<0.05. Results: E-MTA elicits a noticeably enhanced production of IL-10 (2.97 fold) and TGF-β (3.12 fold) levels than its parent compound MTA, demonstrating enhanced anti-inflammatory and immunomodulatory potential while Ketac-Endo and Sealapex showed lesser relative gene fold values Conclusion: It has been concluded that E-MTA exhibits more enhanced bioactivity and capacity to improve the RCT outcome due to reduced inflammation and increased tissue regeneration than Ketac-Endo and Sealapex. https://ojs.zu.edu.pk/pjmd/article/view/3420Endoseal MTAKetac-EndoSealapexIL-10TGF-ΒTissue Repair |
| spellingShingle | Ayesha Imtiaz Kiran Nayyar Khadija Akhtar Muhammad Yousuf Ali Naresh kumar Ehsan Ul Haq Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem Cells Pakistan Journal of Medicine and Dentistry Endoseal MTA Ketac-Endo Sealapex IL-10 TGF-Β Tissue Repair |
| title | Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem Cells |
| title_full | Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem Cells |
| title_fullStr | Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem Cells |
| title_full_unstemmed | Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem Cells |
| title_short | Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem Cells |
| title_sort | comparative analysis of the anti inflammatory effects of e mta ketac e and sealapex on human periodontal ligament stem cells |
| topic | Endoseal MTA Ketac-Endo Sealapex IL-10 TGF-Β Tissue Repair |
| url | https://ojs.zu.edu.pk/pjmd/article/view/3420 |
| work_keys_str_mv | AT ayeshaimtiaz comparativeanalysisoftheantiinflammatoryeffectsofemtaketaceandsealapexonhumanperiodontalligamentstemcells AT kirannayyar comparativeanalysisoftheantiinflammatoryeffectsofemtaketaceandsealapexonhumanperiodontalligamentstemcells AT khadijaakhtar comparativeanalysisoftheantiinflammatoryeffectsofemtaketaceandsealapexonhumanperiodontalligamentstemcells AT muhammadyousufali comparativeanalysisoftheantiinflammatoryeffectsofemtaketaceandsealapexonhumanperiodontalligamentstemcells AT nareshkumar comparativeanalysisoftheantiinflammatoryeffectsofemtaketaceandsealapexonhumanperiodontalligamentstemcells AT ehsanulhaq comparativeanalysisoftheantiinflammatoryeffectsofemtaketaceandsealapexonhumanperiodontalligamentstemcells |