Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs
The expression of chemokines (CCL-2 and CXCL-8) and cytokines (IL-1α, IL-1β, IL-6, TNF-α, and IL-10) was evaluated by RT-qPCR in colostrum-deprived pigs vaccinated and challenged with Haemophilus parasuis serovar 5. Two vaccines containing native proteins with affinity to porcine transferrin (NPAP...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/132432 |
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| author | R. Frandoloso S. Martínez-Martínez E. F. Rodríguez-Ferri S. Yubero D. Rodríguez-Lázaro M. Hernández C. B. Gutiérrez-Martín |
| author_facet | R. Frandoloso S. Martínez-Martínez E. F. Rodríguez-Ferri S. Yubero D. Rodríguez-Lázaro M. Hernández C. B. Gutiérrez-Martín |
| author_sort | R. Frandoloso |
| collection | DOAJ |
| description | The expression of chemokines (CCL-2 and CXCL-8) and cytokines (IL-1α, IL-1β, IL-6, TNF-α, and IL-10) was evaluated by RT-qPCR in colostrum-deprived pigs vaccinated and challenged with Haemophilus parasuis serovar 5. Two vaccines containing native proteins with affinity to porcine transferrin (NPAPTim and NPAPTit) were tested, along with two control groups: one inoculated with PBS instead of antigen (challenge group (CHG)), and another one nonimmunized and noninfected (blank group). The use of NPAPTim and NPAPTit resulted in complete protection against H. parasuis (no clinical signs and/or lesions), and both vaccines were capable of avoiding the expression of the proinflammatory molecules to levels similar to physiological values in blank group. However, overexpression of all proinflammatory molecules was observed in CHG group, mainly in the target infection tissues (brain, lungs, and spleen). High expression of CCL-2, CXCL-8, IL-1α, IL-1β, and IL-6 can be considered one of the characteristics of H. parasuis infection by serovar 5. |
| format | Article |
| id | doaj-art-ff759b82c5d8414593c36632ab1234c1 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-ff759b82c5d8414593c36632ab1234c12025-02-03T01:12:23ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/132432132432Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in PigsR. Frandoloso0S. Martínez-Martínez1E. F. Rodríguez-Ferri2S. Yubero3D. Rodríguez-Lázaro4M. Hernández5C. B. Gutiérrez-Martín6Unidad de Microbiología e Inmunología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24007 León, SpainUnidad de Microbiología e Inmunología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24007 León, SpainUnidad de Microbiología e Inmunología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24007 León, SpainUnidad de Microbiología e Inmunología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24007 León, SpainInstituto Tecnológico Agrario de Castilla y León (ITACyL), 47071 Valladolid, SpainInstituto Tecnológico Agrario de Castilla y León (ITACyL), 47071 Valladolid, SpainUnidad de Microbiología e Inmunología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24007 León, SpainThe expression of chemokines (CCL-2 and CXCL-8) and cytokines (IL-1α, IL-1β, IL-6, TNF-α, and IL-10) was evaluated by RT-qPCR in colostrum-deprived pigs vaccinated and challenged with Haemophilus parasuis serovar 5. Two vaccines containing native proteins with affinity to porcine transferrin (NPAPTim and NPAPTit) were tested, along with two control groups: one inoculated with PBS instead of antigen (challenge group (CHG)), and another one nonimmunized and noninfected (blank group). The use of NPAPTim and NPAPTit resulted in complete protection against H. parasuis (no clinical signs and/or lesions), and both vaccines were capable of avoiding the expression of the proinflammatory molecules to levels similar to physiological values in blank group. However, overexpression of all proinflammatory molecules was observed in CHG group, mainly in the target infection tissues (brain, lungs, and spleen). High expression of CCL-2, CXCL-8, IL-1α, IL-1β, and IL-6 can be considered one of the characteristics of H. parasuis infection by serovar 5.http://dx.doi.org/10.1155/2013/132432 |
| spellingShingle | R. Frandoloso S. Martínez-Martínez E. F. Rodríguez-Ferri S. Yubero D. Rodríguez-Lázaro M. Hernández C. B. Gutiérrez-Martín Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs Clinical and Developmental Immunology |
| title | Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs |
| title_full | Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs |
| title_fullStr | Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs |
| title_full_unstemmed | Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs |
| title_short | Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs |
| title_sort | haemophilus parasuis subunit vaccines based on native proteins with affinity to porcine transferrin prevent the expression of proinflammatory chemokines and cytokines in pigs |
| url | http://dx.doi.org/10.1155/2013/132432 |
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