The relationship between deltamethrin-induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptome
BackgroundDeltamethrin (DM) is a broad-spectrum insecticide that is widely used to control agricultural pests. Recently, DM has posed a potential threat to the health of infants and young children, and this is because of the environmental and food pollution that is caused by the extensive use of DM....
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1526705/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832590858272112640 |
|---|---|
| author | Chunyu Liu Yantong Guo Xue Zhang Hongsong Chen Maomao Han Han Wang Jieyao Niu Jinfei He Jingfeng Yang Wu Dong Jiangdong Xue |
| author_facet | Chunyu Liu Yantong Guo Xue Zhang Hongsong Chen Maomao Han Han Wang Jieyao Niu Jinfei He Jingfeng Yang Wu Dong Jiangdong Xue |
| author_sort | Chunyu Liu |
| collection | DOAJ |
| description | BackgroundDeltamethrin (DM) is a broad-spectrum insecticide that is widely used to control agricultural pests. Recently, DM has posed a potential threat to the health of infants and young children, and this is because of the environmental and food pollution that is caused by the extensive use of DM.MethodsIn this study, zebrafish (Danio rerio) embryos were used as experimental animals to quantify the behavioral changes of larvae induced by DM and explore the relationship between DM and acetylcholinesterase activity.ResultsThe results showed that DM caused an increase in coiling movement, heart rate, and apoptosis in the brain in early zebrafish embryos or larvae. It also caused a decrease in the expression of acetylcholinesterase-associated genes and the activity of acetylcholinesterase, which also led to an increase in the acetylcholinesterase content. The transcriptome data also showed that low concentration DM induced acetylcholine-related gene signaling pathways. The above results suggest that low doses of DM may induce neurodegeneration because DM exposure inhibits acetylcholinesterase, leading to brain cell apoptosis and behavioral changes in the zebrafish embryos or larvae. Micro-injection of zebrafish embryos at the 2–4 cell stage knocked down or overexpressed the acetylcholinesterase gene showed that the behavior and enzyme activity of zebrafish had some effects.ConclusionThis study explored the relationship between acetylcholinesterase and the change in zebrafish behavior caused by DM exposure to provide a basis for the treatment of DM poisoning in the aquaculture. |
| format | Article |
| id | doaj-art-ff694d1a0d914f8698e3bd3b1292d656 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-ff694d1a0d914f8698e3bd3b1292d6562025-01-23T06:56:42ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.15267051526705The relationship between deltamethrin-induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptomeChunyu LiuYantong GuoXue ZhangHongsong ChenMaomao HanHan WangJieyao NiuJinfei HeJingfeng YangWu DongJiangdong XueBackgroundDeltamethrin (DM) is a broad-spectrum insecticide that is widely used to control agricultural pests. Recently, DM has posed a potential threat to the health of infants and young children, and this is because of the environmental and food pollution that is caused by the extensive use of DM.MethodsIn this study, zebrafish (Danio rerio) embryos were used as experimental animals to quantify the behavioral changes of larvae induced by DM and explore the relationship between DM and acetylcholinesterase activity.ResultsThe results showed that DM caused an increase in coiling movement, heart rate, and apoptosis in the brain in early zebrafish embryos or larvae. It also caused a decrease in the expression of acetylcholinesterase-associated genes and the activity of acetylcholinesterase, which also led to an increase in the acetylcholinesterase content. The transcriptome data also showed that low concentration DM induced acetylcholine-related gene signaling pathways. The above results suggest that low doses of DM may induce neurodegeneration because DM exposure inhibits acetylcholinesterase, leading to brain cell apoptosis and behavioral changes in the zebrafish embryos or larvae. Micro-injection of zebrafish embryos at the 2–4 cell stage knocked down or overexpressed the acetylcholinesterase gene showed that the behavior and enzyme activity of zebrafish had some effects.ConclusionThis study explored the relationship between acetylcholinesterase and the change in zebrafish behavior caused by DM exposure to provide a basis for the treatment of DM poisoning in the aquaculture.https://www.frontiersin.org/articles/10.3389/fvets.2024.1526705/fulldeltamethrinzebrafish embryosneurotoxicitybehavioral changesacetylcholinesterase |
| spellingShingle | Chunyu Liu Yantong Guo Xue Zhang Hongsong Chen Maomao Han Han Wang Jieyao Niu Jinfei He Jingfeng Yang Wu Dong Jiangdong Xue The relationship between deltamethrin-induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptome Frontiers in Veterinary Science deltamethrin zebrafish embryos neurotoxicity behavioral changes acetylcholinesterase |
| title | The relationship between deltamethrin-induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptome |
| title_full | The relationship between deltamethrin-induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptome |
| title_fullStr | The relationship between deltamethrin-induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptome |
| title_full_unstemmed | The relationship between deltamethrin-induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptome |
| title_short | The relationship between deltamethrin-induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptome |
| title_sort | relationship between deltamethrin induced behavioral changes and acetylcholinesterase activity in zebrafish embryos or larvae based on transcriptome |
| topic | deltamethrin zebrafish embryos neurotoxicity behavioral changes acetylcholinesterase |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1526705/full |
| work_keys_str_mv | AT chunyuliu therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT yantongguo therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT xuezhang therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT hongsongchen therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT maomaohan therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT hanwang therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT jieyaoniu therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT jinfeihe therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT jingfengyang therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT wudong therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT jiangdongxue therelationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT chunyuliu relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT yantongguo relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT xuezhang relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT hongsongchen relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT maomaohan relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT hanwang relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT jieyaoniu relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT jinfeihe relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT jingfengyang relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT wudong relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome AT jiangdongxue relationshipbetweendeltamethrininducedbehavioralchangesandacetylcholinesteraseactivityinzebrafishembryosorlarvaebasedontranscriptome |