Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesis
A novel selenium- (Se-) hyperaccumulating plant, Cardamine hupingshanesis, accumulating Se as a form of SeCys2, was discovered in Enshi, Hubei, China, which could not be explained by present selenocysteine methyltransferase (SMT) theory. However, it is interesting to investigate if rhizosphere bacte...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/108562 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551436223774720 |
|---|---|
| author | Xinzhao Tong Linxi Yuan Lei Luo Xuebin Yin |
| author_facet | Xinzhao Tong Linxi Yuan Lei Luo Xuebin Yin |
| author_sort | Xinzhao Tong |
| collection | DOAJ |
| description | A novel selenium- (Se-) hyperaccumulating plant, Cardamine hupingshanesis, accumulating Se as a form of SeCys2, was discovered in Enshi, Hubei, China, which could not be explained by present selenocysteine methyltransferase (SMT) theory. However, it is interesting to investigate if rhizosphere bacteria play some roles during SeCys2 accumulation. Here, one Se-tolerant rhizosphere strain, Microbacterium oxydans, was isolated from C. hupingshanesis. Phylogenetic analysis and 16S rRNA gene sequences determined the strain as a kind of Gram positive bacillus and belonged to the family Brevibacterium frigoritolerans. Furthermore, Se tolerance test indicated the strain could grow in extreme high Se level of 15.0 mg Se L−1. When exposed to 1.5 mg Se L−1, SeCys2 was the predominant Se species in the bacteria, consistent with the Se species in C. hupingshanesis. This coincidence might reveal that this strain played some positive effect in SeCys2 accumulation of C. hupingshanesis. Moreover, when exposed to 1.5 mg Se L−1 or 15.0 mg Se L−1, As absorption diminished in the logarithmic phase. In contrast, As absorption increased when exposed to 7.5 mg Se L−1, indicating As metabolism processes could be affected by Se on this strain. The present study provided a sight on the role of rhizosphere bacteria during Se accumulation for Se-hyperaccumulating plant. |
| format | Article |
| id | doaj-art-ff5910c3b24e4c899d43c7ef7f32194b |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ff5910c3b24e4c899d43c7ef7f32194b2025-02-03T06:01:29ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/108562108562Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesisXinzhao Tong0Linxi Yuan1Lei Luo2Xuebin Yin3Advanced Lab for Selenium and Human Health, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou, Jiangsu 215123, ChinaAdvanced Lab for Selenium and Human Health, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou, Jiangsu 215123, ChinaJiangsu Bio-Engineering Centre on Selenium, Suzhou, Jiangsu 215123, ChinaAdvanced Lab for Selenium and Human Health, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou, Jiangsu 215123, ChinaA novel selenium- (Se-) hyperaccumulating plant, Cardamine hupingshanesis, accumulating Se as a form of SeCys2, was discovered in Enshi, Hubei, China, which could not be explained by present selenocysteine methyltransferase (SMT) theory. However, it is interesting to investigate if rhizosphere bacteria play some roles during SeCys2 accumulation. Here, one Se-tolerant rhizosphere strain, Microbacterium oxydans, was isolated from C. hupingshanesis. Phylogenetic analysis and 16S rRNA gene sequences determined the strain as a kind of Gram positive bacillus and belonged to the family Brevibacterium frigoritolerans. Furthermore, Se tolerance test indicated the strain could grow in extreme high Se level of 15.0 mg Se L−1. When exposed to 1.5 mg Se L−1, SeCys2 was the predominant Se species in the bacteria, consistent with the Se species in C. hupingshanesis. This coincidence might reveal that this strain played some positive effect in SeCys2 accumulation of C. hupingshanesis. Moreover, when exposed to 1.5 mg Se L−1 or 15.0 mg Se L−1, As absorption diminished in the logarithmic phase. In contrast, As absorption increased when exposed to 7.5 mg Se L−1, indicating As metabolism processes could be affected by Se on this strain. The present study provided a sight on the role of rhizosphere bacteria during Se accumulation for Se-hyperaccumulating plant.http://dx.doi.org/10.1155/2014/108562 |
| spellingShingle | Xinzhao Tong Linxi Yuan Lei Luo Xuebin Yin Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesis The Scientific World Journal |
| title | Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesis |
| title_full | Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesis |
| title_fullStr | Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesis |
| title_full_unstemmed | Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesis |
| title_short | Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesis |
| title_sort | characterization of a selenium tolerant rhizosphere strain from a novel se hyperaccumulating plant cardamine hupingshanesis |
| url | http://dx.doi.org/10.1155/2014/108562 |
| work_keys_str_mv | AT xinzhaotong characterizationofaseleniumtolerantrhizospherestrainfromanovelsehyperaccumulatingplantcardaminehupingshanesis AT linxiyuan characterizationofaseleniumtolerantrhizospherestrainfromanovelsehyperaccumulatingplantcardaminehupingshanesis AT leiluo characterizationofaseleniumtolerantrhizospherestrainfromanovelsehyperaccumulatingplantcardaminehupingshanesis AT xuebinyin characterizationofaseleniumtolerantrhizospherestrainfromanovelsehyperaccumulatingplantcardaminehupingshanesis |