Synthesis of nano-liposomes containing Mentha piperita essential oil and investigating their stability, antimicrobial, antioxidant, and antiproliferative properties
Objective(s): Considering the problems of using medicinal plants in the treatment of diseases and the role of nanotechnology in reducing these challenges, the present research was conducted with the aim of preparing nano-liposomes containing Mentha piperita essential oil and investigating their phys...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2025-01-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_25106_b4f6748303b53a2dcac7cff2fcaf7ec3.pdf |
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| author | Mohammad Majdizadeh Sanaz Akbarzadeh Hussein H Al-Turnachy Mahdie Hemati Milad Akhlaghi Bibi Fatemeh Haghiralsadat Fatemeh Oroojalian |
| author_facet | Mohammad Majdizadeh Sanaz Akbarzadeh Hussein H Al-Turnachy Mahdie Hemati Milad Akhlaghi Bibi Fatemeh Haghiralsadat Fatemeh Oroojalian |
| author_sort | Mohammad Majdizadeh |
| collection | DOAJ |
| description | Objective(s): Considering the problems of using medicinal plants in the treatment of diseases and the role of nanotechnology in reducing these challenges, the present research was conducted with the aim of preparing nano-liposomes containing Mentha piperita essential oil and investigating their physicochemical characteristics. Materials and Methods: Four nano-liposome formulations containing essential oil were prepared using cholesterol and phosphatidylcholine by thin layer method. Encapsulation efficiency, size, zeta potential, and essential oil release were measured in all formulations. The appropriate formulation was selected to investigate the morphology of the particles, their interaction between nano-liposomes and essential oil, toxicity, and antioxidant, antibacterial, and antifungal properties. Then, the stability of the selected formulation was checked for 120 days. Results: Formulation F1 was selected with an encapsulation efficiency of 62.12%, nano-particle size of 121 nm, and zeta potential of -21.8 mV. In this formulation, no interaction between nano-liposomes and essential oil was observed, and the spherical shape and two-layer nature of the nanoparticles were confirmed. Nano-liposomes with and without essential oil caused little toxicity to normal HFF cells and in all concentrations compared to free essential oil, they had more toxicity on MCF-7 cancer cells and higher antioxidant properties. The anti-proliferative effects of nano-liposomes on some microorganisms were higher than the free essential oil. Also, there were slight changes in some physicochemical properties of nanoparticles during 120 days. Conclusion: Considering the suitable physicochemical properties of nano-liposomes containing essential oil and their anti-proliferative effects, these nano-systems can be suggested for further research in the field of cancer and microbial diseases. |
| format | Article |
| id | doaj-art-ff51cdbb7e1c4e72b4c59ae4acce2dc2 |
| institution | DOAJ |
| issn | 2322-3049 2322-5904 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj-art-ff51cdbb7e1c4e72b4c59ae4acce2dc22025-08-20T03:09:12ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042025-01-0112112213910.22038/nmj.2024.79435.196025106Synthesis of nano-liposomes containing Mentha piperita essential oil and investigating their stability, antimicrobial, antioxidant, and antiproliferative propertiesMohammad Majdizadeh0Sanaz Akbarzadeh1Hussein H Al-Turnachy2Mahdie Hemati3Milad Akhlaghi4Bibi Fatemeh Haghiralsadat5Fatemeh Oroojalian6Nano-Biotech Foresight Company Biotechnology Campus, Science & Technology Park of Yazd, Yazd, IranDepartment of Chemistry, Yasouj University, Yasouj, IranDepartment of Biology, Faculty of Science, University of Kufa, Iraq5Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranBiotechnology Research Center, International Campus, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranBiotechnology Research Center, International Campus, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, IranNatural Products and Medicinal Plants Research Center North Khorasan University of Medical Sciences Bojnūrd, IranObjective(s): Considering the problems of using medicinal plants in the treatment of diseases and the role of nanotechnology in reducing these challenges, the present research was conducted with the aim of preparing nano-liposomes containing Mentha piperita essential oil and investigating their physicochemical characteristics. Materials and Methods: Four nano-liposome formulations containing essential oil were prepared using cholesterol and phosphatidylcholine by thin layer method. Encapsulation efficiency, size, zeta potential, and essential oil release were measured in all formulations. The appropriate formulation was selected to investigate the morphology of the particles, their interaction between nano-liposomes and essential oil, toxicity, and antioxidant, antibacterial, and antifungal properties. Then, the stability of the selected formulation was checked for 120 days. Results: Formulation F1 was selected with an encapsulation efficiency of 62.12%, nano-particle size of 121 nm, and zeta potential of -21.8 mV. In this formulation, no interaction between nano-liposomes and essential oil was observed, and the spherical shape and two-layer nature of the nanoparticles were confirmed. Nano-liposomes with and without essential oil caused little toxicity to normal HFF cells and in all concentrations compared to free essential oil, they had more toxicity on MCF-7 cancer cells and higher antioxidant properties. The anti-proliferative effects of nano-liposomes on some microorganisms were higher than the free essential oil. Also, there were slight changes in some physicochemical properties of nanoparticles during 120 days. Conclusion: Considering the suitable physicochemical properties of nano-liposomes containing essential oil and their anti-proliferative effects, these nano-systems can be suggested for further research in the field of cancer and microbial diseases.https://nmj.mums.ac.ir/article_25106_b4f6748303b53a2dcac7cff2fcaf7ec3.pdfantioxidantantibacterialbreast canceressential oil.mentha piperitanano-liposomes |
| spellingShingle | Mohammad Majdizadeh Sanaz Akbarzadeh Hussein H Al-Turnachy Mahdie Hemati Milad Akhlaghi Bibi Fatemeh Haghiralsadat Fatemeh Oroojalian Synthesis of nano-liposomes containing Mentha piperita essential oil and investigating their stability, antimicrobial, antioxidant, and antiproliferative properties Nanomedicine Journal antioxidant antibacterial breast cancer essential oil.mentha piperita nano-liposomes |
| title | Synthesis of nano-liposomes containing Mentha piperita essential oil and investigating their stability, antimicrobial, antioxidant, and antiproliferative properties |
| title_full | Synthesis of nano-liposomes containing Mentha piperita essential oil and investigating their stability, antimicrobial, antioxidant, and antiproliferative properties |
| title_fullStr | Synthesis of nano-liposomes containing Mentha piperita essential oil and investigating their stability, antimicrobial, antioxidant, and antiproliferative properties |
| title_full_unstemmed | Synthesis of nano-liposomes containing Mentha piperita essential oil and investigating their stability, antimicrobial, antioxidant, and antiproliferative properties |
| title_short | Synthesis of nano-liposomes containing Mentha piperita essential oil and investigating their stability, antimicrobial, antioxidant, and antiproliferative properties |
| title_sort | synthesis of nano liposomes containing mentha piperita essential oil and investigating their stability antimicrobial antioxidant and antiproliferative properties |
| topic | antioxidant antibacterial breast cancer essential oil.mentha piperita nano-liposomes |
| url | https://nmj.mums.ac.ir/article_25106_b4f6748303b53a2dcac7cff2fcaf7ec3.pdf |
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