Effect of Ribavirin on Hepatitis A Virus Replication In Vitro
The effect of ribavirin on fetal Rhesus monkey kidney cells (FRhK-4) acutely or chronically infected with hepatitis A virus was studied. The effect of ribavirin on hepatitis A virus yield as detected by radioimmunoassay in acutely infected FRhK-4 cells was dependent on hepatitis A virus inoculum dos...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1992-01-01
|
| Series: | Canadian Journal of Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/1992/531837 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560916970864640 |
|---|---|
| author | Rabindra K Chaudhary Anton P Andonov |
| author_facet | Rabindra K Chaudhary Anton P Andonov |
| author_sort | Rabindra K Chaudhary |
| collection | DOAJ |
| description | The effect of ribavirin on fetal Rhesus monkey kidney cells (FRhK-4) acutely or chronically infected with hepatitis A virus was studied. The effect of ribavirin on hepatitis A virus yield as detected by radioimmunoassay in acutely infected FRhK-4 cells was dependent on hepatitis A virus inoculum dose. Treatment with 100 μg/mL ribavirin completely inhibited hepatitis A virus growth in cultures infected with 100 to 800 tissue culture infectious dose 50 (TCID50) hepatitis A virus, but inocula of 800 to 1600 TCID50 resulted in limited production of virus. The effect was time dependent and required more than 96 h of treatment to inhibit the virus completely. Ribavirin was less effective in treating cells persistently infected with hepatitis A virus, although there was significant inhibition of hepatitis A virus (82%) in persistently infected cells as well. Ribavirin had some inhibitory effect on cell growth; treatment with 25, 50 or 100 μg/mL ribavirin reduced cell growth by approximately 0, 20 and 40%, respectively. |
| format | Article |
| id | doaj-art-ff3c0c660da24f1dac22b6a18f88d720 |
| institution | Kabale University |
| issn | 1180-2332 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases |
| spelling | doaj-art-ff3c0c660da24f1dac22b6a18f88d7202025-02-03T01:26:31ZengWileyCanadian Journal of Infectious Diseases1180-23321992-01-0132677010.1155/1992/531837Effect of Ribavirin on Hepatitis A Virus Replication In VitroRabindra K Chaudhary0Anton P Andonov1Laboratory for Viral Hepatitis, National Laboratory for Special Pathogens, Bureau of Microbiology, Laboratory Centre for Disease Control, Health and Welfare Canada, Ottawa, Ontario, CanadaLaboratory for Viral Hepatitis, National Laboratory for Special Pathogens, Bureau of Microbiology, Laboratory Centre for Disease Control, Health and Welfare Canada, Ottawa, Ontario, CanadaThe effect of ribavirin on fetal Rhesus monkey kidney cells (FRhK-4) acutely or chronically infected with hepatitis A virus was studied. The effect of ribavirin on hepatitis A virus yield as detected by radioimmunoassay in acutely infected FRhK-4 cells was dependent on hepatitis A virus inoculum dose. Treatment with 100 μg/mL ribavirin completely inhibited hepatitis A virus growth in cultures infected with 100 to 800 tissue culture infectious dose 50 (TCID50) hepatitis A virus, but inocula of 800 to 1600 TCID50 resulted in limited production of virus. The effect was time dependent and required more than 96 h of treatment to inhibit the virus completely. Ribavirin was less effective in treating cells persistently infected with hepatitis A virus, although there was significant inhibition of hepatitis A virus (82%) in persistently infected cells as well. Ribavirin had some inhibitory effect on cell growth; treatment with 25, 50 or 100 μg/mL ribavirin reduced cell growth by approximately 0, 20 and 40%, respectively.http://dx.doi.org/10.1155/1992/531837 |
| spellingShingle | Rabindra K Chaudhary Anton P Andonov Effect of Ribavirin on Hepatitis A Virus Replication In Vitro Canadian Journal of Infectious Diseases |
| title | Effect of Ribavirin on Hepatitis A Virus Replication In Vitro |
| title_full | Effect of Ribavirin on Hepatitis A Virus Replication In Vitro |
| title_fullStr | Effect of Ribavirin on Hepatitis A Virus Replication In Vitro |
| title_full_unstemmed | Effect of Ribavirin on Hepatitis A Virus Replication In Vitro |
| title_short | Effect of Ribavirin on Hepatitis A Virus Replication In Vitro |
| title_sort | effect of ribavirin on hepatitis a virus replication in vitro |
| url | http://dx.doi.org/10.1155/1992/531837 |
| work_keys_str_mv | AT rabindrakchaudhary effectofribavirinonhepatitisavirusreplicationinvitro AT antonpandonov effectofribavirinonhepatitisavirusreplicationinvitro |