HLA-G high-expressor 3’UTR markers are linked to gastric cancer development and survival
Abstract Gastric cancer ranks fifth in both world prevalence and lethality, with a 5-year survival of less than 30%. HLA-G, a non-classical class I HLA gene, has emerged as a potential marker for cancer susceptibility and prognosis due to its immunomodulatory properties. Its level of expression is r...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2024-11-01
|
| Series: | Cancer Immunology, Immunotherapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s00262-024-03771-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832571696627843072 |
|---|---|
| author | Christian Vaquero-Yuste Ignacio Juarez Marta Molina-Alejandre Elisa María Molanes-López Alberto Gutiérrez-Calvo Adela López-García Inmaculada Lasa Remedios Gómez Antonio Arnaiz-Villena Jose Manuel Martín-Villa |
| author_facet | Christian Vaquero-Yuste Ignacio Juarez Marta Molina-Alejandre Elisa María Molanes-López Alberto Gutiérrez-Calvo Adela López-García Inmaculada Lasa Remedios Gómez Antonio Arnaiz-Villena Jose Manuel Martín-Villa |
| author_sort | Christian Vaquero-Yuste |
| collection | DOAJ |
| description | Abstract Gastric cancer ranks fifth in both world prevalence and lethality, with a 5-year survival of less than 30%. HLA-G, a non-classical class I HLA gene, has emerged as a potential marker for cancer susceptibility and prognosis due to its immunomodulatory properties. Its level of expression is regulated by polymorphisms in the 3’ untranslated region (3’UTR) polymorphisms, which form various combined haplotypes (UTR-1 to -9). In this study, we examined HLA-G 3’UTR polymorphisms in paired tissue samples from 111 patients with gastric adenocarcinoma and 119 healthy controls. Polymorphism analysis was performed using PCR and Sanger sequencing, followed by statistical analysis using SNPStats software. Survival analysis was conducted using Kaplan–Meier curves and multivariate Cox regression models. High-expressor HLA-G 3’UTR haplotypes (UTR-1 and UTR-6) were significantly associated with gastric cancer susceptibility, indicating a potential role in tumor immune evasion. Additionally, the 14 base pair insertion/deletion polymorphism (14 bp I/D) emerged as a prognostic marker, with D/D genotype carriers showing lower survival rates compared to I/D and I/I genotype carriers. Our study highlights the clinical relevance of HLA-G polymorphisms in gastric cancer, suggesting their potential as prognostic markers and therapeutic targets. Further elucidation of HLA-G-related pathways could lead to personalized treatment strategies and improved patient outcomes in gastric cancer. |
| format | Article |
| id | doaj-art-ff35db722aef44888cbab112f50e9e65 |
| institution | Kabale University |
| issn | 1432-0851 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Springer |
| record_format | Article |
| series | Cancer Immunology, Immunotherapy |
| spelling | doaj-art-ff35db722aef44888cbab112f50e9e652025-02-02T12:26:33ZengSpringerCancer Immunology, Immunotherapy1432-08512024-11-017411610.1007/s00262-024-03771-wHLA-G high-expressor 3’UTR markers are linked to gastric cancer development and survivalChristian Vaquero-Yuste0Ignacio Juarez1Marta Molina-Alejandre2Elisa María Molanes-López3Alberto Gutiérrez-Calvo4Adela López-García5Inmaculada Lasa6Remedios Gómez7Antonio Arnaiz-Villena8Jose Manuel Martín-Villa9Departamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, Universidad Complutense de MadridDepartamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, Universidad Complutense de MadridDepartamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, Universidad Complutense de MadridDepartamento de Estadística e Investigación Operativa, Facultad de Medicina, Universidad Complutense de MadridServicio de Cirugía General y Aparato Digestivo, Hospital Universitario Príncipe de AsturiasServicio de Cirugía General y Aparato Digestivo, Hospital Universitario Príncipe de AsturiasServicio de Cirugía General y Aparato Digestivo, Hospital Universitario Príncipe de AsturiasServicio de Cirugía General y Aparato Digestivo, Hospital Universitario Príncipe de AsturiasDepartamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, Universidad Complutense de MadridDepartamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, Universidad Complutense de MadridAbstract Gastric cancer ranks fifth in both world prevalence and lethality, with a 5-year survival of less than 30%. HLA-G, a non-classical class I HLA gene, has emerged as a potential marker for cancer susceptibility and prognosis due to its immunomodulatory properties. Its level of expression is regulated by polymorphisms in the 3’ untranslated region (3’UTR) polymorphisms, which form various combined haplotypes (UTR-1 to -9). In this study, we examined HLA-G 3’UTR polymorphisms in paired tissue samples from 111 patients with gastric adenocarcinoma and 119 healthy controls. Polymorphism analysis was performed using PCR and Sanger sequencing, followed by statistical analysis using SNPStats software. Survival analysis was conducted using Kaplan–Meier curves and multivariate Cox regression models. High-expressor HLA-G 3’UTR haplotypes (UTR-1 and UTR-6) were significantly associated with gastric cancer susceptibility, indicating a potential role in tumor immune evasion. Additionally, the 14 base pair insertion/deletion polymorphism (14 bp I/D) emerged as a prognostic marker, with D/D genotype carriers showing lower survival rates compared to I/D and I/I genotype carriers. Our study highlights the clinical relevance of HLA-G polymorphisms in gastric cancer, suggesting their potential as prognostic markers and therapeutic targets. Further elucidation of HLA-G-related pathways could lead to personalized treatment strategies and improved patient outcomes in gastric cancer.https://doi.org/10.1007/s00262-024-03771-wHLA-G antigensStomach neoplasmPrognosisHaplotypesClinical relevanceTumor escape |
| spellingShingle | Christian Vaquero-Yuste Ignacio Juarez Marta Molina-Alejandre Elisa María Molanes-López Alberto Gutiérrez-Calvo Adela López-García Inmaculada Lasa Remedios Gómez Antonio Arnaiz-Villena Jose Manuel Martín-Villa HLA-G high-expressor 3’UTR markers are linked to gastric cancer development and survival Cancer Immunology, Immunotherapy HLA-G antigens Stomach neoplasm Prognosis Haplotypes Clinical relevance Tumor escape |
| title | HLA-G high-expressor 3’UTR markers are linked to gastric cancer development and survival |
| title_full | HLA-G high-expressor 3’UTR markers are linked to gastric cancer development and survival |
| title_fullStr | HLA-G high-expressor 3’UTR markers are linked to gastric cancer development and survival |
| title_full_unstemmed | HLA-G high-expressor 3’UTR markers are linked to gastric cancer development and survival |
| title_short | HLA-G high-expressor 3’UTR markers are linked to gastric cancer development and survival |
| title_sort | hla g high expressor 3 utr markers are linked to gastric cancer development and survival |
| topic | HLA-G antigens Stomach neoplasm Prognosis Haplotypes Clinical relevance Tumor escape |
| url | https://doi.org/10.1007/s00262-024-03771-w |
| work_keys_str_mv | AT christianvaqueroyuste hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT ignaciojuarez hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT martamolinaalejandre hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT elisamariamolaneslopez hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT albertogutierrezcalvo hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT adelalopezgarcia hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT inmaculadalasa hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT remediosgomez hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT antonioarnaizvillena hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival AT josemanuelmartinvilla hlaghighexpressor3utrmarkersarelinkedtogastriccancerdevelopmentandsurvival |