Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress
Abiotic stresses comprise all nonliving factors, such as soil salinity, drought, extreme temperatures, and metal toxicity, posing a serious threat to agriculture and affecting the plant production around the world. Peanut (Arachis hypogaea L.) is one of the most important crops for vegetable oil, pr...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2020/6524093 |
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| author | Hao Zhang Xiaobo Zhao Quanxi Sun Caixia Yan Juan Wang Cuiling Yuan Chunjuan Li Shihua Shan Fengzhen Liu |
| author_facet | Hao Zhang Xiaobo Zhao Quanxi Sun Caixia Yan Juan Wang Cuiling Yuan Chunjuan Li Shihua Shan Fengzhen Liu |
| author_sort | Hao Zhang |
| collection | DOAJ |
| description | Abiotic stresses comprise all nonliving factors, such as soil salinity, drought, extreme temperatures, and metal toxicity, posing a serious threat to agriculture and affecting the plant production around the world. Peanut (Arachis hypogaea L.) is one of the most important crops for vegetable oil, proteins, minerals, and vitamins in the world. Therefore, it is of importance to understand the molecular mechanism of peanut against salt stress. Six transcriptome sequencing libraries including 24-hour salt treatments and control samples were constructed from the young leaves of peanut. A comprehensive analysis between two groups detected 3,425 differentially expressed genes (DEGs) including 2,013 upregulated genes and 1,412 downregulated genes. Of these DEGs, 141 transcription factors (TFs) mainly consisting of MYB, AP2/ERF, WRKY, bHLH, and HSF were identified in response to salinity stress. Further, GO categories of the DEGs highly related to regulation of cell growth, cell periphery, sustained external encapsulating structure, cell wall organization or biogenesis, antioxidant activity, and peroxidase activity were significantly enriched for upregulated DEGs. The function of downregulated DEGs was mainly enriched in regulation of metabolic processes, oxidoreductase activity, and catalytic activity. Fourteen DEGs with response to salt tolerance were validated by real-time PCR. Taken together, the identification of DEGs’ response to salt tolerance of cultivated peanut will provide a solid foundation for improving salt-tolerant peanut genetic manipulation in the future. |
| format | Article |
| id | doaj-art-ff2c5269f6564990a217758c34f308c2 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-ff2c5269f6564990a217758c34f308c22025-02-03T06:05:13ZengWileyInternational Journal of Genomics2314-436X2314-43782020-01-01202010.1155/2020/65240936524093Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt StressHao Zhang0Xiaobo Zhao1Quanxi Sun2Caixia Yan3Juan Wang4Cuiling Yuan5Chunjuan Li6Shihua Shan7Fengzhen Liu8State Key Laboratory of Crop Biology and College of Agronomy, Shandong Agricultural University, Tai’an, Shandong 271018, ChinaShandong Peanut Research Institute, Qingdao, Shandong 266000, ChinaShandong Peanut Research Institute, Qingdao, Shandong 266000, ChinaShandong Peanut Research Institute, Qingdao, Shandong 266000, ChinaShandong Peanut Research Institute, Qingdao, Shandong 266000, ChinaShandong Peanut Research Institute, Qingdao, Shandong 266000, ChinaShandong Peanut Research Institute, Qingdao, Shandong 266000, ChinaShandong Peanut Research Institute, Qingdao, Shandong 266000, ChinaState Key Laboratory of Crop Biology and College of Agronomy, Shandong Agricultural University, Tai’an, Shandong 271018, ChinaAbiotic stresses comprise all nonliving factors, such as soil salinity, drought, extreme temperatures, and metal toxicity, posing a serious threat to agriculture and affecting the plant production around the world. Peanut (Arachis hypogaea L.) is one of the most important crops for vegetable oil, proteins, minerals, and vitamins in the world. Therefore, it is of importance to understand the molecular mechanism of peanut against salt stress. Six transcriptome sequencing libraries including 24-hour salt treatments and control samples were constructed from the young leaves of peanut. A comprehensive analysis between two groups detected 3,425 differentially expressed genes (DEGs) including 2,013 upregulated genes and 1,412 downregulated genes. Of these DEGs, 141 transcription factors (TFs) mainly consisting of MYB, AP2/ERF, WRKY, bHLH, and HSF were identified in response to salinity stress. Further, GO categories of the DEGs highly related to regulation of cell growth, cell periphery, sustained external encapsulating structure, cell wall organization or biogenesis, antioxidant activity, and peroxidase activity were significantly enriched for upregulated DEGs. The function of downregulated DEGs was mainly enriched in regulation of metabolic processes, oxidoreductase activity, and catalytic activity. Fourteen DEGs with response to salt tolerance were validated by real-time PCR. Taken together, the identification of DEGs’ response to salt tolerance of cultivated peanut will provide a solid foundation for improving salt-tolerant peanut genetic manipulation in the future.http://dx.doi.org/10.1155/2020/6524093 |
| spellingShingle | Hao Zhang Xiaobo Zhao Quanxi Sun Caixia Yan Juan Wang Cuiling Yuan Chunjuan Li Shihua Shan Fengzhen Liu Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress International Journal of Genomics |
| title | Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress |
| title_full | Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress |
| title_fullStr | Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress |
| title_full_unstemmed | Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress |
| title_short | Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress |
| title_sort | comparative transcriptome analysis reveals molecular defensive mechanism of arachis hypogaea in response to salt stress |
| url | http://dx.doi.org/10.1155/2020/6524093 |
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