Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil
The bacteriological quality of crabs from three different mangroves (Itaóca, Suruí, and Piedade) from Rio de Janeiro state, Brazil, was investigated using conventional and molecular methods. The results revealed high counts for total coliforms in meat and hepatopancreas samples. PCR analyses identif...
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Wiley
2016-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2016/7825031 |
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| author | M. C. N. Carvalho M. M. Jayme G. S. Arenazio F. V. Araújo S. G. F. Leite E. M. Del Aguila |
| author_facet | M. C. N. Carvalho M. M. Jayme G. S. Arenazio F. V. Araújo S. G. F. Leite E. M. Del Aguila |
| author_sort | M. C. N. Carvalho |
| collection | DOAJ |
| description | The bacteriological quality of crabs from three different mangroves (Itaóca, Suruí, and Piedade) from Rio de Janeiro state, Brazil, was investigated using conventional and molecular methods. The results revealed high counts for total coliforms in meat and hepatopancreas samples. PCR analyses identified 25 Escherichia coli colonies in the Itaóca, Piedade, and Suruí samples, detecting 13 enterotoxigenic colonies and 9 enteroaggregative colonies. Respectively, 12, 11, and 21 Vibrio parahaemolyticus strains were detected in the Itaóca, Piedade, and Suruí samples. Two V. cholerae strains were detected in the Piedade samples. The E. coli strains isolated in the present study showed resistance to gentamicin. E. coli strains from the Piedade samples showed 33% resistance to chloramphenicol and the strains also showed multiresistance to several antimicrobial agents with a MAR index ranging from 0.12 to 0.31. Vibrio strains from Piedade, Itaóca, and Suruí showed 86%, 78%, and 85% resistance, respectively, to ampicillin. The isolated Vibrio strains showed multiresistance to several antimicrobial agents, with a MAR index ranging from 0.12 to 0.25. The presence of these organisms in crab meat is an indication of microbial contamination, which may pose health risks to consumers when improperly cooked. |
| format | Article |
| id | doaj-art-ff0eaa7d7dfa44349f711bb0846fd554 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-ff0eaa7d7dfa44349f711bb0846fd5542025-02-03T01:00:29ZengWileyInternational Journal of Microbiology1687-918X1687-91982016-01-01201610.1155/2016/78250317825031Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, BrazilM. C. N. Carvalho0M. M. Jayme1G. S. Arenazio2F. V. Araújo3S. G. F. Leite4E. M. Del Aguila5Instituto de Química, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira 149, Bloco A, Cidade Universitária, 21949-909 Rio de Janeiro, RJ, BrazilFaculdade de Ciências Médicas, Universidade do Estado do Rio do Janeiro (UERJ), Avenida Professor Manoel de Abreu 444, 2° andar, Vila Isabel, 20550-170 Rio de Janeiro, RJ, BrazilFaculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro (FFP-UERJ), Rua Dr. Francisco Portela 1470, Patronato, 24435-005 São Gonçalo, RJ, BrazilFaculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro (FFP-UERJ), Rua Dr. Francisco Portela 1470, Patronato, 24435-005 São Gonçalo, RJ, BrazilEscola de Química, Universidade Federal do Rio de Janeiro (UFRJ), Avenida Athos da Silveira 149, Bloco E, Cidade Universitária, 21949-909 Rio de Janeiro, RJ, BrazilInstituto de Química, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira 149, Bloco A, Cidade Universitária, 21949-909 Rio de Janeiro, RJ, BrazilThe bacteriological quality of crabs from three different mangroves (Itaóca, Suruí, and Piedade) from Rio de Janeiro state, Brazil, was investigated using conventional and molecular methods. The results revealed high counts for total coliforms in meat and hepatopancreas samples. PCR analyses identified 25 Escherichia coli colonies in the Itaóca, Piedade, and Suruí samples, detecting 13 enterotoxigenic colonies and 9 enteroaggregative colonies. Respectively, 12, 11, and 21 Vibrio parahaemolyticus strains were detected in the Itaóca, Piedade, and Suruí samples. Two V. cholerae strains were detected in the Piedade samples. The E. coli strains isolated in the present study showed resistance to gentamicin. E. coli strains from the Piedade samples showed 33% resistance to chloramphenicol and the strains also showed multiresistance to several antimicrobial agents with a MAR index ranging from 0.12 to 0.31. Vibrio strains from Piedade, Itaóca, and Suruí showed 86%, 78%, and 85% resistance, respectively, to ampicillin. The isolated Vibrio strains showed multiresistance to several antimicrobial agents, with a MAR index ranging from 0.12 to 0.25. The presence of these organisms in crab meat is an indication of microbial contamination, which may pose health risks to consumers when improperly cooked.http://dx.doi.org/10.1155/2016/7825031 |
| spellingShingle | M. C. N. Carvalho M. M. Jayme G. S. Arenazio F. V. Araújo S. G. F. Leite E. M. Del Aguila Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil International Journal of Microbiology |
| title | Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil |
| title_full | Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil |
| title_fullStr | Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil |
| title_full_unstemmed | Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil |
| title_short | Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil |
| title_sort | microbiological quality assessment by pcr and its antibiotic susceptibility in mangrove crabs ucides cordatus from guanabara bay rio de janeiro brazil |
| url | http://dx.doi.org/10.1155/2016/7825031 |
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