Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon Carcinomas
Expression of the γ2 chain at the invasive front of different tumors has indicated an important role for laminin-5 in cell migration during tumor invasion and tissue remodeling. As there is considerable need for reliable invasion and prognostic markers we evaluated the correlation of laminin-5 γ2 ch...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2001/137404 |
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| author | C. Lenander J. K. Habermann Å. Öst B. Nilsson H. Schimmelpenning K. Tryggvason G. Auer |
| author_facet | C. Lenander J. K. Habermann Å. Öst B. Nilsson H. Schimmelpenning K. Tryggvason G. Auer |
| author_sort | C. Lenander |
| collection | DOAJ |
| description | Expression of the γ2 chain at the invasive front of different tumors has indicated an important role for laminin-5 in cell migration during tumor invasion and tissue remodeling. As there is considerable need for reliable invasion and prognostic markers we evaluated the correlation of laminin-5 γ2 chain expression with clinicopathologic parameters and patient survival in 93 primary colon carcinomas. Epithelial cells of normal mucosa were consistently negative for staining. In contrast, positive cytoplasmic staining was observed in 89 tumors (96%). Twenty-four (26%) cases were scored as sparse, 34 (37%) as moderate, and 31 (33%) as frequent γ2 chain expression. There was a significant association of laminin-5 γ2 chain expression and local invasiveness of colon carcinomas according to Dukes stage (A-C) (p = 0.001) and tumor budding (p < 0.001). A statistical significance could also be noted in decreasing tumor differentiation (p < 0.001) and correlation to tumor size (p = 0.032). No correlation was observed to tumor site. Univariate analysis identified laminin-5 (p = 0.010), tumor differentiation (p = 0.006) and Dukes grade (p < 0.001) as significant variables in predicting prognosis. However, by multivariate analyses, this study could not demonstrate that laminin-5 γ2 chain expression is an independent predictive factor for survival. The results indicate that laminin-5 γ2 chain expression is up-regulated during the progression of human colon cancer and that it plays a role in the aggressiveness of these tumors. Demonstration of laminin-5 γ2 chain positivity also facilitates detection of individual cells or minor cell clusters invading the surrounding stroma. |
| format | Article |
| id | doaj-art-fefa58e4d4fb4d14be8258c05ff5f88e |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-fefa58e4d4fb4d14be8258c05ff5f88e2025-02-03T05:57:36ZengWileyAnalytical Cellular Pathology0921-89121878-36512001-01-0122420120910.1155/2001/137404Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon CarcinomasC. Lenander0J. K. Habermann1Å. Öst2B. Nilsson3H. Schimmelpenning4K. Tryggvason5G. Auer6Department of Surgery, Ersta Hospital, Stockholm, SwedenDepartment of Surgery, Medical University of Lübeck, GermanyMedilab patologi, Täby, SwedenDepartment of Oncology, Epidemiological Unit, Radiumhemmet, Karolinska Hospital, Stockholm, SwedenDepartment of Surgery, Medical University of Lübeck, GermanyDivision of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, SwedenDivision of Cellular and Molecular Analysis, Department of Oncology and Pathology, Karolinska Institutet, Stockholm, SwedenExpression of the γ2 chain at the invasive front of different tumors has indicated an important role for laminin-5 in cell migration during tumor invasion and tissue remodeling. As there is considerable need for reliable invasion and prognostic markers we evaluated the correlation of laminin-5 γ2 chain expression with clinicopathologic parameters and patient survival in 93 primary colon carcinomas. Epithelial cells of normal mucosa were consistently negative for staining. In contrast, positive cytoplasmic staining was observed in 89 tumors (96%). Twenty-four (26%) cases were scored as sparse, 34 (37%) as moderate, and 31 (33%) as frequent γ2 chain expression. There was a significant association of laminin-5 γ2 chain expression and local invasiveness of colon carcinomas according to Dukes stage (A-C) (p = 0.001) and tumor budding (p < 0.001). A statistical significance could also be noted in decreasing tumor differentiation (p < 0.001) and correlation to tumor size (p = 0.032). No correlation was observed to tumor site. Univariate analysis identified laminin-5 (p = 0.010), tumor differentiation (p = 0.006) and Dukes grade (p < 0.001) as significant variables in predicting prognosis. However, by multivariate analyses, this study could not demonstrate that laminin-5 γ2 chain expression is an independent predictive factor for survival. The results indicate that laminin-5 γ2 chain expression is up-regulated during the progression of human colon cancer and that it plays a role in the aggressiveness of these tumors. Demonstration of laminin-5 γ2 chain positivity also facilitates detection of individual cells or minor cell clusters invading the surrounding stroma.http://dx.doi.org/10.1155/2001/137404 |
| spellingShingle | C. Lenander J. K. Habermann Å. Öst B. Nilsson H. Schimmelpenning K. Tryggvason G. Auer Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon Carcinomas Analytical Cellular Pathology |
| title | Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon Carcinomas |
| title_full | Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon Carcinomas |
| title_fullStr | Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon Carcinomas |
| title_full_unstemmed | Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon Carcinomas |
| title_short | Laminin-5 γ2 Chain Expression Correlates with Unfavorable Prognosis in Colon Carcinomas |
| title_sort | laminin 5 γ2 chain expression correlates with unfavorable prognosis in colon carcinomas |
| url | http://dx.doi.org/10.1155/2001/137404 |
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