VALIDATION OF METHOD FOR YERSINIA ENTEROCOLITICA ISOLATION FROM RAW MATERIALS OF ANIMAL ORIGIN

Upward trend in the number of human yersiniosis cases, caused by bacterium Yersinia enterocolitica, is globally observed nowadays. This microorganism is widely spread in the environment, able to persist for prolonged periods in animal products and propagate under low temperatures. Basic infection so...

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Main Authors: I. V. Borodkina, N. B. Shadrova, O. V. Pruntova, S. I. Danilchenko
Format: Article
Language:English
Published: Da Vinci Media 2019-06-01
Series:Ветеринария сегодня
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Online Access:https://veterinary.arriah.ru/jour/article/view/418
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author I. V. Borodkina
N. B. Shadrova
O. V. Pruntova
S. I. Danilchenko
author_facet I. V. Borodkina
N. B. Shadrova
O. V. Pruntova
S. I. Danilchenko
author_sort I. V. Borodkina
collection DOAJ
description Upward trend in the number of human yersiniosis cases, caused by bacterium Yersinia enterocolitica, is globally observed nowadays. This microorganism is widely spread in the environment, able to persist for prolonged periods in animal products and propagate under low temperatures. Basic infection sources are meat and meat products. In order to isolate Yersinia enterocolitica from food and feed samples horizontal method for the detection pursuant to GOST ISO 10273-2013 was used. It was noted, that Yersinia enterocolitica isolation is associated with certain difficulties, because the sample contains only small quantities of the agent and only the use of special techniques allows removing the concurrent microflora. It was proposed to use cold enrich­ment (4 ± 1) °C of the test material before conventional technique is started. The technique was validated pursuant to GOST ISO 16140-2011. As a result, it was established that validated method for Yersinia enterocolitica bacteria detection in food products, performed at the Microbiology Laboratory, is specific. The method sensitivity is 10 CFU/cm3. Intralaboratory reproducibility and repeatability were confirmed by relevant tests. Additional culture step at (4 ± 1) °C allows complete inhibition of non-psychrophilic microorganisms’ growth.
format Article
id doaj-art-fec3241ae76643cd85aa9ee437537f53
institution Kabale University
issn 2304-196X
2658-6959
language English
publishDate 2019-06-01
publisher Da Vinci Media
record_format Article
series Ветеринария сегодня
spelling doaj-art-fec3241ae76643cd85aa9ee437537f532025-02-06T09:52:07ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592019-06-0102606510.29326/2304-196X-2019-2-29-60-65411VALIDATION OF METHOD FOR YERSINIA ENTEROCOLITICA ISOLATION FROM RAW MATERIALS OF ANIMAL ORIGINI. V. Borodkina0N. B. Shadrova1O. V. Pruntova2S. I. Danilchenko3FGBI “ARRIAH” Branch in Republic of CrimeaFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI “ARRIAH” Branch in Republic of CrimeaUpward trend in the number of human yersiniosis cases, caused by bacterium Yersinia enterocolitica, is globally observed nowadays. This microorganism is widely spread in the environment, able to persist for prolonged periods in animal products and propagate under low temperatures. Basic infection sources are meat and meat products. In order to isolate Yersinia enterocolitica from food and feed samples horizontal method for the detection pursuant to GOST ISO 10273-2013 was used. It was noted, that Yersinia enterocolitica isolation is associated with certain difficulties, because the sample contains only small quantities of the agent and only the use of special techniques allows removing the concurrent microflora. It was proposed to use cold enrich­ment (4 ± 1) °C of the test material before conventional technique is started. The technique was validated pursuant to GOST ISO 16140-2011. As a result, it was established that validated method for Yersinia enterocolitica bacteria detection in food products, performed at the Microbiology Laboratory, is specific. The method sensitivity is 10 CFU/cm3. Intralaboratory reproducibility and repeatability were confirmed by relevant tests. Additional culture step at (4 ± 1) °C allows complete inhibition of non-psychrophilic microorganisms’ growth.https://veterinary.arriah.ru/jour/article/view/418yersiniosisyersinia enterocoliticapsychrophilic microorganismscold enrichment
spellingShingle I. V. Borodkina
N. B. Shadrova
O. V. Pruntova
S. I. Danilchenko
VALIDATION OF METHOD FOR YERSINIA ENTEROCOLITICA ISOLATION FROM RAW MATERIALS OF ANIMAL ORIGIN
Ветеринария сегодня
yersiniosis
yersinia enterocolitica
psychrophilic microorganisms
cold enrichment
title VALIDATION OF METHOD FOR YERSINIA ENTEROCOLITICA ISOLATION FROM RAW MATERIALS OF ANIMAL ORIGIN
title_full VALIDATION OF METHOD FOR YERSINIA ENTEROCOLITICA ISOLATION FROM RAW MATERIALS OF ANIMAL ORIGIN
title_fullStr VALIDATION OF METHOD FOR YERSINIA ENTEROCOLITICA ISOLATION FROM RAW MATERIALS OF ANIMAL ORIGIN
title_full_unstemmed VALIDATION OF METHOD FOR YERSINIA ENTEROCOLITICA ISOLATION FROM RAW MATERIALS OF ANIMAL ORIGIN
title_short VALIDATION OF METHOD FOR YERSINIA ENTEROCOLITICA ISOLATION FROM RAW MATERIALS OF ANIMAL ORIGIN
title_sort validation of method for yersinia enterocolitica isolation from raw materials of animal origin
topic yersiniosis
yersinia enterocolitica
psychrophilic microorganisms
cold enrichment
url https://veterinary.arriah.ru/jour/article/view/418
work_keys_str_mv AT ivborodkina validationofmethodforyersiniaenterocoliticaisolationfromrawmaterialsofanimalorigin
AT nbshadrova validationofmethodforyersiniaenterocoliticaisolationfromrawmaterialsofanimalorigin
AT ovpruntova validationofmethodforyersiniaenterocoliticaisolationfromrawmaterialsofanimalorigin
AT sidanilchenko validationofmethodforyersiniaenterocoliticaisolationfromrawmaterialsofanimalorigin