Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh Pandemic
CTXΦ is a lysogenic filamentous phage that carries the genes encoding cholera toxin (<i>ctxAB</i>), the main virulence factor of <i>Vibrio cholerae</i>. The toxigenic conversion of environmental <i>V. cholerae</i> strains through CTXΦ lysogenic infection is crucia...
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2024-09-01
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| author | Xiaorui Li Yu Han Wenxuan Zhao Yue Xiao Siyu Huang Zhenpeng Li Fenxia Fan Weili Liang Biao Kan |
| author_facet | Xiaorui Li Yu Han Wenxuan Zhao Yue Xiao Siyu Huang Zhenpeng Li Fenxia Fan Weili Liang Biao Kan |
| author_sort | Xiaorui Li |
| collection | DOAJ |
| description | CTXΦ is a lysogenic filamentous phage that carries the genes encoding cholera toxin (<i>ctxAB</i>), the main virulence factor of <i>Vibrio cholerae</i>. The toxigenic conversion of environmental <i>V. cholerae</i> strains through CTXΦ lysogenic infection is crucial for the emergence of new pathogenic clones. A special allelic form of CTXΦ, called pre-CTXΦ, is a precursor of CTXΦ and without <i>ctxAB</i>. Different members of the pre-CTXΦ and CTXΦ families are distinguished by the sequence of the transcriptional repressor-coding gene <i>rstR</i>. Multiple <i>rstR</i> alleles can coexist within a single strain, demonstrating the diverse structure and complex genomic integration patterns of CTXΦ/pre-CTXΦ prophage on the chromosome. Exploration of the diversity and co-integration patterns of CTXΦ/pre-CTXΦ prophages in <i>V. cholerae</i> can help to understand the evolution of this phage family. In this study, 21 <i>V. cholerae</i> strains, which were shown to carry the CTXΦ/pre-CTXΦ prophages as opposed to typical CTX<sup>ET</sup>Φ-RS1 structure, were selected from approximately 1000 strains with diverse genomes. We identified two CTXΦ members and six pre-CTXΦ members with distinct <i>rstR</i> alleles, revealing complex chromosomal DNA integration patterns and arrangements of different prophages in these strains. Promoter activity assays showed that the transcriptional repressor RstR protected against CTXΦ superinfection by preventing the replication and integration of CTXΦ/pre-CTXΦ phages containing the same <i>rstR</i> allele, supporting the co-integration of the diverse CTXΦ/pre-CTXΦ members observed. The numbers and types of prophages and their co-integration arrangements in serogroup O139 strains were more complex than those in serogroup O1 strains. Also, these CTXΦ/pre-CTXΦ members were shown to present the bloom period of the CTXΦ/pre-CTXΦ family during wave 2 of the seventh cholera pandemic. Together, these analyses deepen our comprehension of the genetic variation of CTXΦ and pre-CTXΦ and provide insights into the evolution of the CTXΦ/pre-CTXΦ family in the seventh cholera pandemic. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-09-01 |
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| spelling | doaj-art-feb531539c29425f9ec4815de6559cb52025-02-03T13:27:43ZengMDPI AGMicroorganisms2076-26072024-09-011210193510.3390/microorganisms12101935Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh PandemicXiaorui Li0Yu Han1Wenxuan Zhao2Yue Xiao3Siyu Huang4Zhenpeng Li5Fenxia Fan6Weili Liang7Biao Kan8National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changbai Road 155, Changping District, Beijing 102206, ChinaCTXΦ is a lysogenic filamentous phage that carries the genes encoding cholera toxin (<i>ctxAB</i>), the main virulence factor of <i>Vibrio cholerae</i>. The toxigenic conversion of environmental <i>V. cholerae</i> strains through CTXΦ lysogenic infection is crucial for the emergence of new pathogenic clones. A special allelic form of CTXΦ, called pre-CTXΦ, is a precursor of CTXΦ and without <i>ctxAB</i>. Different members of the pre-CTXΦ and CTXΦ families are distinguished by the sequence of the transcriptional repressor-coding gene <i>rstR</i>. Multiple <i>rstR</i> alleles can coexist within a single strain, demonstrating the diverse structure and complex genomic integration patterns of CTXΦ/pre-CTXΦ prophage on the chromosome. Exploration of the diversity and co-integration patterns of CTXΦ/pre-CTXΦ prophages in <i>V. cholerae</i> can help to understand the evolution of this phage family. In this study, 21 <i>V. cholerae</i> strains, which were shown to carry the CTXΦ/pre-CTXΦ prophages as opposed to typical CTX<sup>ET</sup>Φ-RS1 structure, were selected from approximately 1000 strains with diverse genomes. We identified two CTXΦ members and six pre-CTXΦ members with distinct <i>rstR</i> alleles, revealing complex chromosomal DNA integration patterns and arrangements of different prophages in these strains. Promoter activity assays showed that the transcriptional repressor RstR protected against CTXΦ superinfection by preventing the replication and integration of CTXΦ/pre-CTXΦ phages containing the same <i>rstR</i> allele, supporting the co-integration of the diverse CTXΦ/pre-CTXΦ members observed. The numbers and types of prophages and their co-integration arrangements in serogroup O139 strains were more complex than those in serogroup O1 strains. Also, these CTXΦ/pre-CTXΦ members were shown to present the bloom period of the CTXΦ/pre-CTXΦ family during wave 2 of the seventh cholera pandemic. Together, these analyses deepen our comprehension of the genetic variation of CTXΦ and pre-CTXΦ and provide insights into the evolution of the CTXΦ/pre-CTXΦ family in the seventh cholera pandemic.https://www.mdpi.com/2076-2607/12/10/1935<i>V. cholerae</i>CTXΦpre-CTXΦRstRgenome structure |
| spellingShingle | Xiaorui Li Yu Han Wenxuan Zhao Yue Xiao Siyu Huang Zhenpeng Li Fenxia Fan Weili Liang Biao Kan Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh Pandemic Microorganisms <i>V. cholerae</i> CTXΦ pre-CTXΦ RstR genome structure |
| title | Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh Pandemic |
| title_full | Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh Pandemic |
| title_fullStr | Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh Pandemic |
| title_full_unstemmed | Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh Pandemic |
| title_short | Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in <em>Vibrio cholerae</em> from Seventh Pandemic |
| title_sort | diversity and complexity of ctxφ and pre ctxφ families in em vibrio cholerae em from seventh pandemic |
| topic | <i>V. cholerae</i> CTXΦ pre-CTXΦ RstR genome structure |
| url | https://www.mdpi.com/2076-2607/12/10/1935 |
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