Optimization of fermentation process of high-yield sterol Candida tropicalis and preparation of sterol-rich modified milk
In this study, using defatted milk powder and walnut oil as main raw materials, the freeze-dried sterol powder was prepared with high-yield sterol Candida tropicalis 1253, Lactobacillus bulgaricus and Streptococcus thermophilus as fermentation strains. On this basis, the sterol-rich modified milk wa...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2024-08-01
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| Series: | Zhongguo niangzao |
| Subjects: | |
| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-8-133.pdf |
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| Summary: | In this study, using defatted milk powder and walnut oil as main raw materials, the freeze-dried sterol powder was prepared with high-yield sterol Candida tropicalis 1253, Lactobacillus bulgaricus and Streptococcus thermophilus as fermentation strains. On this basis, the sterol-rich modified milk was prepared. Using sterol yield as evaluation index, the fermentation process of strain 1253 was optimized by single factor tests and response surface tests. The quality and nutrition of sterol-rich modified milk was evaluated comprehensively, and the key differential metabolites and metabolic pathways were analyzed. The results showed that the optimum fermentation technology was culture time 97 h, temperature 30 ℃, inoculum 9.5%, walnut oil addition 4%. Under the optimized conditions, the sterol yield was 0.90 mg/g. The sensory score of sterol-enriched modified milk was (86.44±2.06) and the sterol content was (22.7±0.6) mg/g. Key differential metabolites such as pyridoxal, cholesterol, gamma-aminobutyric acid (GABA), acteoside and citric acid formed the characteristic flavors through enrichment of vitamin B<sub>6</sub> metabolism, pentose phosphate pathway, ethoxylate and dicarboxylate metabolism, fructose and mannose metabolism, and citric acid cycle. |
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| ISSN: | 0254-5071 |