Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α
Obesity is primarily due to excessive energy intake and lipid accumulation, leading to type 2 diabetes. Studies showed radish seed extract (RSE) can impede weight gain in mice, but the mechanism was unclear. We hypothesized that RSE inhibits obesity by stimulating adipocyte browning. Radish seeds we...
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SAGE Publishing
2025-02-01
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| Series: | Journal of Evidence-Based Integrative Medicine |
| Online Access: | https://doi.org/10.1177/2515690X251316760 |
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| author | Yao-Chien Wang MD Yu-An Hsu PhD Sheng-Chun Lin MD Li-Shan Chien MS Jamie Jiin Yi Chen MD Ming Yen Wu MD Hui-Ju Lin MD, PhD Chih-Sheng Chen MD, PhD Yi-Qi Huang MS Yu-Chi Tsai PhD Lei Wan PhD |
| author_facet | Yao-Chien Wang MD Yu-An Hsu PhD Sheng-Chun Lin MD Li-Shan Chien MS Jamie Jiin Yi Chen MD Ming Yen Wu MD Hui-Ju Lin MD, PhD Chih-Sheng Chen MD, PhD Yi-Qi Huang MS Yu-Chi Tsai PhD Lei Wan PhD |
| author_sort | Yao-Chien Wang MD |
| collection | DOAJ |
| description | Obesity is primarily due to excessive energy intake and lipid accumulation, leading to type 2 diabetes. Studies showed radish seed extract (RSE) can impede weight gain in mice, but the mechanism was unclear. We hypothesized that RSE inhibits obesity by stimulating adipocyte browning. Radish seeds were water-extracted, yielding a sulforaphene (SE) concentration of 1.381 ± 0.005 mg/g RSE. In 3T3-L1 adipocyte differentiation experiments, RSE and SE increased the expression of beige adipocyte markers uncoupling protein 1 (UCP1) and peroxisome proliferator-activated receptor-γ coactivator 1-α (PGC1α). In C57BL/6 mice, RSE and SE mitigated weight increase, averted fatty liver, and diminished fat accumulation. In the adipose tissue, we also noted the enhanced browning of white adipocytes through elevated expression of UCP1 and PGC1α. Increased mitochondrial numbers in treated adipocytes supported this effect. Additionally, RSE and SE improved glucose homeostasis and insulin sensitivity in high-fat diet-fed mice, indicating RSE's potential to prevent obesity and diabetes by enhancing adipocyte thermogenesis. |
| format | Article |
| id | doaj-art-fe88ad2f2cb54151aa2fc3beb14ec35c |
| institution | Kabale University |
| issn | 2515-690X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Evidence-Based Integrative Medicine |
| spelling | doaj-art-fe88ad2f2cb54151aa2fc3beb14ec35c2025-02-05T09:03:31ZengSAGE PublishingJournal of Evidence-Based Integrative Medicine2515-690X2025-02-013010.1177/2515690X251316760Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-αYao-Chien Wang MD0Yu-An Hsu PhD1Sheng-Chun Lin MD2Li-Shan Chien MS3Jamie Jiin Yi Chen MD4Ming Yen Wu MD5Hui-Ju Lin MD, PhD6Chih-Sheng Chen MD, PhD7Yi-Qi Huang MS8Yu-Chi Tsai PhD9Lei Wan PhD10 Department of Emergency Medicine, , Taichung, Taiwan School of Chinese Medicine, , Taichung, Taiwan Graduate Institute of Biomedical Sciences, , Taichung, Taiwan Graduate Institute of Biomedical Sciences, , Taichung, Taiwan School of Chinese Medicine, , Taichung, Taiwan School of Chinese Medicine, , Taichung, Taiwan Eye Center, , , Taichung, Taiwan Division of Chinese Medicine, , Taichung, Taiwan School of Chinese Medicine, , Taichung, Taiwan School of Chinese Medicine, , Taichung, Taiwan Department of Medical Laboratory Science and Biotechnology, , Taichung, TaiwanObesity is primarily due to excessive energy intake and lipid accumulation, leading to type 2 diabetes. Studies showed radish seed extract (RSE) can impede weight gain in mice, but the mechanism was unclear. We hypothesized that RSE inhibits obesity by stimulating adipocyte browning. Radish seeds were water-extracted, yielding a sulforaphene (SE) concentration of 1.381 ± 0.005 mg/g RSE. In 3T3-L1 adipocyte differentiation experiments, RSE and SE increased the expression of beige adipocyte markers uncoupling protein 1 (UCP1) and peroxisome proliferator-activated receptor-γ coactivator 1-α (PGC1α). In C57BL/6 mice, RSE and SE mitigated weight increase, averted fatty liver, and diminished fat accumulation. In the adipose tissue, we also noted the enhanced browning of white adipocytes through elevated expression of UCP1 and PGC1α. Increased mitochondrial numbers in treated adipocytes supported this effect. Additionally, RSE and SE improved glucose homeostasis and insulin sensitivity in high-fat diet-fed mice, indicating RSE's potential to prevent obesity and diabetes by enhancing adipocyte thermogenesis.https://doi.org/10.1177/2515690X251316760 |
| spellingShingle | Yao-Chien Wang MD Yu-An Hsu PhD Sheng-Chun Lin MD Li-Shan Chien MS Jamie Jiin Yi Chen MD Ming Yen Wu MD Hui-Ju Lin MD, PhD Chih-Sheng Chen MD, PhD Yi-Qi Huang MS Yu-Chi Tsai PhD Lei Wan PhD Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Journal of Evidence-Based Integrative Medicine |
| title | Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α |
| title_full | Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α |
| title_fullStr | Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α |
| title_full_unstemmed | Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α |
| title_short | Radish Seed Exerts Anti-Diabetic and Obesity-Reducing Effects in Mice by Promoting the Activation of Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α |
| title_sort | radish seed exerts anti diabetic and obesity reducing effects in mice by promoting the activation of uncoupling protein 1 and peroxisome proliferator activated receptor γ coactivator 1 α |
| url | https://doi.org/10.1177/2515690X251316760 |
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