In Situ Aqueous Spice Extract-Based Antifungal Lock Strategy for Salvage of Foley’s Catheter Biofouled with <i>Candida albicans</i> Biofilm Gel

In Situ Aqueous Spice Extract-Based Antifungal Lock Strategy for Salvage of Foley’s Catheter Biofouled with <i>Candida albicans</i> Biofilm Gel

<i>Candida</i> forms a gel-like biofilm in the Foley’s catheter (FC) causing tenacious biofouling and severe urinary tract infections (UTIs). For the first time, a spice extract-based antifungal lock therapy (ALT) has been developed to inhibit the <i>Candida albicans</i> gel...

Full description

Saved in:
Bibliographic Details
Main Authors: Bindu Sadanandan, Vaniyamparambath Vijayalakshmi, Kalidas Shetty, Adithya Rathish, Harshala Shivkumar, Malavika Gundreddy, Nikhil Kumar Kagganti Narendra, Nethra Machamada Devaiah
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Gels
Subjects:
antifungal lock therapy
in situ
Foley’s catheter
gel
biofilm
spice extract
Online Access:https://www.mdpi.com/2310-2861/11/1/23
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<i>Candida</i> forms a gel-like biofilm in the Foley’s catheter (FC) causing tenacious biofouling and severe urinary tract infections (UTIs). For the first time, a spice extract-based antifungal lock therapy (ALT) has been developed to inhibit the <i>Candida albicans</i> gel matrix in FC. Aqueous extracts of garlic, clove, and Indian gooseberry were used as ALT lock solutions and tested against biofilm-forming multidrug-resistant clinical isolates of <i>C. albicans</i>. Reduction in the gel matrices formation in the catheter was confirmed by Point inoculation, MTT assay, CFU, and SEM analysis at 12 and 24 h of incubation. Garlic was effective in controlling both <i>C. albicans</i> M207 and <i>C. albicans</i> S470; however, clove and gooseberry effectively controlled the latter. As evidenced by CFU assay, there were 82.85% and 99.68% reductions in the growth of <i>C. albicans</i> M207 and S470, respectively, at 24 h of incubation. SEM revealed a switch from the biofilm to the yeast mode and a drastic reduction in cell numbers, with mostly clumped or lysed cells. The study will provide an impetus to the development of novel spice extract-based ALT, reducing the selection pressure on the pathogen and lowering antimicrobial resistance. Further research in this area has the potential to leverage clinical applications.
ISSN:2310-2861

Similar Items