Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest Ethiopia
Background. Food handlers with untrimmed finger nails could contribute or serve as a vehicle for the transmission of food poisoning pathogens. Objectives. This study was conducted to determine the prevalence of bacteria and intestinal parasites among food handlers and antibiotic susceptibility profi...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/6532014 |
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| author | Abeba Mengist Yibeltal Aschale Alemayehu Reta |
| author_facet | Abeba Mengist Yibeltal Aschale Alemayehu Reta |
| author_sort | Abeba Mengist |
| collection | DOAJ |
| description | Background. Food handlers with untrimmed finger nails could contribute or serve as a vehicle for the transmission of food poisoning pathogens. Objectives. This study was conducted to determine the prevalence of bacteria and intestinal parasites among food handlers and antibiotic susceptibility profile of the isolated bacteria in Debre Markos University, Ethiopia. Materials and Methods. This laboratory-based cross-sectional study involved 220 food handlers working in food service establishments in Debre Markos University between 1st January 2015 to 31th June 2016. Subjects’ finger nail specimens of both hands were examined microscopically for intestinal parasites. For bacterial isolation, samples were cultured and bacterial species were identified following standard laboratory procedures. Antimicrobial susceptibility test was performed for all bacterial isolates by using Kirby-Bauer disk diffusion method. Results. Of the total 220 subjects examined, 29.5% showed positive culture for different bacterial species from their fingernail contents. Coagulase-negative Staphylococcus was the predominant bacteria species (12.3%) followed by Staphylococcus aureus (5%), E. coli (2.7%), Klebsiella species (2.7%), Enterococcus species (1.8%), Pseudomonas aeruginosa (1.8%), Proteus species (1.4%), Citrobacter species (1.4%), and Serratia species (0.9%). None of the food handlers showed positive culture for Shigella and salmonella and parasites in respect of their finger nail specimens. Isolation of bacteria in finger nail has significant association with finger nail status (P=0.044) and inverse relation with service years (P=0.048). All Staphylococcus aureus and coagulase-negative Staphylococcus species isolates were uniformly susceptible to vancomycin. Only one (9.1%) of Staphylococcus aureus isolates was resistant for methicillin. Conclusion. To prevent the food poisoning pathogens, implementation and adherence to infection are the key practices, specially food handlers with long finger nail harbor food debris, microbial contaminations, and allergens. |
| format | Article |
| id | doaj-art-fe7477def7414178b7d690012d790263 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-fe7477def7414178b7d690012d7902632025-02-03T01:00:28ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932018-01-01201810.1155/2018/65320146532014Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest EthiopiaAbeba Mengist0Yibeltal Aschale1Alemayehu Reta2Department of Medical Laboratory Science, College of Medical and Health Sciences, Debre Markos University, Debre Markos, EthiopiaDepartment of Medical Laboratory Science, College of Medical and Health Sciences, Debre Markos University, Debre Markos, EthiopiaDepartment of Medical Laboratory Science, College of Medical and Health Sciences, Debre Markos University, Debre Markos, EthiopiaBackground. Food handlers with untrimmed finger nails could contribute or serve as a vehicle for the transmission of food poisoning pathogens. Objectives. This study was conducted to determine the prevalence of bacteria and intestinal parasites among food handlers and antibiotic susceptibility profile of the isolated bacteria in Debre Markos University, Ethiopia. Materials and Methods. This laboratory-based cross-sectional study involved 220 food handlers working in food service establishments in Debre Markos University between 1st January 2015 to 31th June 2016. Subjects’ finger nail specimens of both hands were examined microscopically for intestinal parasites. For bacterial isolation, samples were cultured and bacterial species were identified following standard laboratory procedures. Antimicrobial susceptibility test was performed for all bacterial isolates by using Kirby-Bauer disk diffusion method. Results. Of the total 220 subjects examined, 29.5% showed positive culture for different bacterial species from their fingernail contents. Coagulase-negative Staphylococcus was the predominant bacteria species (12.3%) followed by Staphylococcus aureus (5%), E. coli (2.7%), Klebsiella species (2.7%), Enterococcus species (1.8%), Pseudomonas aeruginosa (1.8%), Proteus species (1.4%), Citrobacter species (1.4%), and Serratia species (0.9%). None of the food handlers showed positive culture for Shigella and salmonella and parasites in respect of their finger nail specimens. Isolation of bacteria in finger nail has significant association with finger nail status (P=0.044) and inverse relation with service years (P=0.048). All Staphylococcus aureus and coagulase-negative Staphylococcus species isolates were uniformly susceptible to vancomycin. Only one (9.1%) of Staphylococcus aureus isolates was resistant for methicillin. Conclusion. To prevent the food poisoning pathogens, implementation and adherence to infection are the key practices, specially food handlers with long finger nail harbor food debris, microbial contaminations, and allergens.http://dx.doi.org/10.1155/2018/6532014 |
| spellingShingle | Abeba Mengist Yibeltal Aschale Alemayehu Reta Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest Ethiopia Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest Ethiopia |
| title_full | Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest Ethiopia |
| title_fullStr | Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest Ethiopia |
| title_full_unstemmed | Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest Ethiopia |
| title_short | Bacterial and Parasitic Assessment from Fingernails in Debre Markos, Northwest Ethiopia |
| title_sort | bacterial and parasitic assessment from fingernails in debre markos northwest ethiopia |
| url | http://dx.doi.org/10.1155/2018/6532014 |
| work_keys_str_mv | AT abebamengist bacterialandparasiticassessmentfromfingernailsindebremarkosnorthwestethiopia AT yibeltalaschale bacterialandparasiticassessmentfromfingernailsindebremarkosnorthwestethiopia AT alemayehureta bacterialandparasiticassessmentfromfingernailsindebremarkosnorthwestethiopia |