Antibiofilm Activity of Curcumin and Piperine and Their Synergistic Effects with Antifungals against Candida albicans Clinical Isolates
Background. Candidiasis is the common name for diseases caused by yeast of the genus Candida. Candida albicans is one of the most implicated species in superficial and invasive candidiasis. Antifungals, polyenes, and azoles have been used to treat candidiasis. However, due to the development of anti...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2024/2025557 |
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| author | Ulrich Joël Tsopmene Christian Ramsès Tokam Kuaté Prudence Ngalula Kayoka-Kabongo Borel Ndezo Bisso Anisel Metopa Clautilde Teugwa Mofor Jean Paul Dzoyem |
| author_facet | Ulrich Joël Tsopmene Christian Ramsès Tokam Kuaté Prudence Ngalula Kayoka-Kabongo Borel Ndezo Bisso Anisel Metopa Clautilde Teugwa Mofor Jean Paul Dzoyem |
| author_sort | Ulrich Joël Tsopmene |
| collection | DOAJ |
| description | Background. Candidiasis is the common name for diseases caused by yeast of the genus Candida. Candida albicans is one of the most implicated species in superficial and invasive candidiasis. Antifungals, polyenes, and azoles have been used to treat candidiasis. However, due to the development of antifungal resistance, research of natural substances with potential antifungal effects at low concentrations or combined is also a possibility. Methods. The broth microdilution method was used to evaluate the antifungal activity. The biofilm formation was assessed using the microtiter plate method. The antibiofilm activities were assessed using micro plaque tetrazolium salt assay (MTT). The combination effect of antifungal with natural substances was made using the checkerboard method. Results. Among our isolates, clotrimazole was the most resistant, but amphotericin B was the most effective antifungal. The biofilm was formed by all isolates of C. albicans. Curcumin and piperine displayed antibiofilm activity with minimum biofilm inhibitory concentration (MBIC) and minimum eradicating concentration (MBEC) ranging from 64 to 1024 μg/mL and 256 to 2048 μg/mL. In combination, piperine presented double synergistic effects compared to curcumin with all antifungals tested. Curcumin shows more synergistic effect when combined with polyenes than with azoles. However, piperine shows a more synergistic effect when combined with azoles compared to polyenes. Conclusion. C. albicans was susceptible to curcumin and piperine both on planktonic cells and biofilm. The combination of curcumin and piperine with antifungals has shown synergistic effects against multiresistant clinical isolates of Candida albicans representing an alternative drug research for the treatment of clinical candidiasis. |
| format | Article |
| id | doaj-art-fe5e33336dce40e492bf7afe56873df7 |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-fe5e33336dce40e492bf7afe56873df72025-02-03T07:23:42ZengWileyScientifica2090-908X2024-01-01202410.1155/2024/2025557Antibiofilm Activity of Curcumin and Piperine and Their Synergistic Effects with Antifungals against Candida albicans Clinical IsolatesUlrich Joël Tsopmene0Christian Ramsès Tokam Kuaté1Prudence Ngalula Kayoka-Kabongo2Borel Ndezo Bisso3Anisel Metopa4Clautilde Teugwa Mofor5Jean Paul Dzoyem6Department of BiochemistryDepartment of BiochemistryDepartment of Agriculture and Animal HealthDepartment of BiochemistryDepartment of BiochemistryDepartment of BiochemistryDepartment of BiochemistryBackground. Candidiasis is the common name for diseases caused by yeast of the genus Candida. Candida albicans is one of the most implicated species in superficial and invasive candidiasis. Antifungals, polyenes, and azoles have been used to treat candidiasis. However, due to the development of antifungal resistance, research of natural substances with potential antifungal effects at low concentrations or combined is also a possibility. Methods. The broth microdilution method was used to evaluate the antifungal activity. The biofilm formation was assessed using the microtiter plate method. The antibiofilm activities were assessed using micro plaque tetrazolium salt assay (MTT). The combination effect of antifungal with natural substances was made using the checkerboard method. Results. Among our isolates, clotrimazole was the most resistant, but amphotericin B was the most effective antifungal. The biofilm was formed by all isolates of C. albicans. Curcumin and piperine displayed antibiofilm activity with minimum biofilm inhibitory concentration (MBIC) and minimum eradicating concentration (MBEC) ranging from 64 to 1024 μg/mL and 256 to 2048 μg/mL. In combination, piperine presented double synergistic effects compared to curcumin with all antifungals tested. Curcumin shows more synergistic effect when combined with polyenes than with azoles. However, piperine shows a more synergistic effect when combined with azoles compared to polyenes. Conclusion. C. albicans was susceptible to curcumin and piperine both on planktonic cells and biofilm. The combination of curcumin and piperine with antifungals has shown synergistic effects against multiresistant clinical isolates of Candida albicans representing an alternative drug research for the treatment of clinical candidiasis.http://dx.doi.org/10.1155/2024/2025557 |
| spellingShingle | Ulrich Joël Tsopmene Christian Ramsès Tokam Kuaté Prudence Ngalula Kayoka-Kabongo Borel Ndezo Bisso Anisel Metopa Clautilde Teugwa Mofor Jean Paul Dzoyem Antibiofilm Activity of Curcumin and Piperine and Their Synergistic Effects with Antifungals against Candida albicans Clinical Isolates Scientifica |
| title | Antibiofilm Activity of Curcumin and Piperine and Their Synergistic Effects with Antifungals against Candida albicans Clinical Isolates |
| title_full | Antibiofilm Activity of Curcumin and Piperine and Their Synergistic Effects with Antifungals against Candida albicans Clinical Isolates |
| title_fullStr | Antibiofilm Activity of Curcumin and Piperine and Their Synergistic Effects with Antifungals against Candida albicans Clinical Isolates |
| title_full_unstemmed | Antibiofilm Activity of Curcumin and Piperine and Their Synergistic Effects with Antifungals against Candida albicans Clinical Isolates |
| title_short | Antibiofilm Activity of Curcumin and Piperine and Their Synergistic Effects with Antifungals against Candida albicans Clinical Isolates |
| title_sort | antibiofilm activity of curcumin and piperine and their synergistic effects with antifungals against candida albicans clinical isolates |
| url | http://dx.doi.org/10.1155/2024/2025557 |
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