Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis
Forty Wistar rats were divided into 5 groups, including the control group, the acute pancreatitis group (AP group, induced by intraperitoneal injections of caerulein), and the AP group treated with baicalin, the AP group treated with LPS, and the AP group treated with LPS and baicalin. Pathological...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/26295 |
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| author | Dongbo Xue Weihui Zhang Yingmei Zhang Haiyang Wang Biao Zheng Xingye Shi |
| author_facet | Dongbo Xue Weihui Zhang Yingmei Zhang Haiyang Wang Biao Zheng Xingye Shi |
| author_sort | Dongbo Xue |
| collection | DOAJ |
| description | Forty Wistar rats were divided into 5 groups, including the control group, the acute pancreatitis group (AP group, induced by intraperitoneal injections of caerulein), and the AP group treated with baicalin, the AP group treated with LPS, and the AP group treated with LPS and baicalin. Pathological damage of pancreatic tissue was scored with hematoxylin and eosin (HE) staining. The mRNA expression of TNF-α was measured with semiquantitative RT-PCR, and activation of NF-κB was detected with flow cytometry assay. It was shown in the results that the expression of TNF-α mRNA, activation of NF-κB, and pathological score of AP group were all obviously higher than those of control group (P<.01). In AP group treated with LPS, further rise of these values were observed (P<.01). In the AP group treated with baicalin, activation of NF-κB decreased (P<.05), and expression of TNF-α mRNA also obviously decreased (P<.01), while pancreatic pathological damage was alleviated at the same time (P<.01); similar results were observed in AP group treated with LPS and baicalin (P<.01), which indicated that baicalin might be applied to inhibit NF-κB activating and TNF-α expressing so as to treat AP. |
| format | Article |
| id | doaj-art-fe4860990c264afb958de23d5197f59e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-fe4860990c264afb958de23d5197f59e2025-02-03T01:02:05ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/2629526295Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute PancreatitisDongbo Xue0Weihui Zhang1Yingmei Zhang2Haiyang Wang3Biao Zheng4Xingye Shi5Department of General Surgery, First Clinical College, Harbin Medical University, Harbin 150001, ChinaDepartment of General Surgery, First Clinical College, Harbin Medical University, Harbin 150001, ChinaCentral Laboratory, First Clinical College, Harbin Medical University, Harbin 150001, ChinaDepartment of General Surgery, First Clinical College, Harbin Medical University, Harbin 150001, ChinaDepartment of General Surgery, First Clinical College, Harbin Medical University, Harbin 150001, ChinaDepartment of General Surgery, First Clinical College, Harbin Medical University, Harbin 150001, ChinaForty Wistar rats were divided into 5 groups, including the control group, the acute pancreatitis group (AP group, induced by intraperitoneal injections of caerulein), and the AP group treated with baicalin, the AP group treated with LPS, and the AP group treated with LPS and baicalin. Pathological damage of pancreatic tissue was scored with hematoxylin and eosin (HE) staining. The mRNA expression of TNF-α was measured with semiquantitative RT-PCR, and activation of NF-κB was detected with flow cytometry assay. It was shown in the results that the expression of TNF-α mRNA, activation of NF-κB, and pathological score of AP group were all obviously higher than those of control group (P<.01). In AP group treated with LPS, further rise of these values were observed (P<.01). In the AP group treated with baicalin, activation of NF-κB decreased (P<.05), and expression of TNF-α mRNA also obviously decreased (P<.01), while pancreatic pathological damage was alleviated at the same time (P<.01); similar results were observed in AP group treated with LPS and baicalin (P<.01), which indicated that baicalin might be applied to inhibit NF-κB activating and TNF-α expressing so as to treat AP.http://dx.doi.org/10.1155/MI/2006/26295 |
| spellingShingle | Dongbo Xue Weihui Zhang Yingmei Zhang Haiyang Wang Biao Zheng Xingye Shi Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis Mediators of Inflammation |
| title | Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis |
| title_full | Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis |
| title_fullStr | Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis |
| title_full_unstemmed | Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis |
| title_short | Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis |
| title_sort | adjusting effects of baicalin for nuclear factor κb and tumor necrosis factor α on rats with caerulein induced acute pancreatitis |
| url | http://dx.doi.org/10.1155/MI/2006/26295 |
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