Multi-Component Characterization and Quality Evaluation Strategy of <i>Sarcandrae</i> Herba by Combining Dual-Column Tandem HPLC Fingerprint and UPLC-Q-TOF-MS/MS
A dual-column tandem mode was used to establish the fingerprints of <i>Sarcandrae</i> herb from different origins, and their chemical compositions were characterized by UPLC-Q-TOF-MS/MS, which provided an experimental basis for the establishment of a rapid and efficient method for the ov...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/8/1825 |
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| Summary: | A dual-column tandem mode was used to establish the fingerprints of <i>Sarcandrae</i> herb from different origins, and their chemical compositions were characterized by UPLC-Q-TOF-MS/MS, which provided an experimental basis for the establishment of a rapid and efficient method for the overall quality control of <i>Sarcandrae</i> herba. For the first time, nine common components were identified from the <i>Sarcandrae</i> herba herbs of 24 origins, which were neochlorogenic acid, chlorogenic acid, 4-caffeoylquinic acid, eleutheroside B1, quercetin-3-O-β-D-glucuronide, neoastilbin, astilbin, isofraxidin, and rosmarinic acid, respectively. A total of 92 compounds were identified by liquid mass spectrometry. The quality of the <i>Sarcandrae</i> herb from 24 origins was analyzed by similarity evaluation, principal component analysis, and cluster analysis, and the chemical components of <i>Sarcandrae</i> herba were identified by UPLC-Q-TOF-MS/MS. The results showed that the overall analysis based on fingerprinting and mass spectrometry could differentiate the origins of the herbs. |
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| ISSN: | 1420-3049 |