The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats
Objective. To investigate the effects of low dose ethanol feeding in diabetic rats and analyze its underlying mechanisms. Methods. Male Sprague-Dawley rats were divided into 4 groups: control (Con), diabetes at 4 weeks (DM4W), diabetes at 8 weeks (DM8W), and EtOH + DM8W. After 8 weeks, hemodynamic p...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/3834283 |
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| author | Ying Yu Xian-Jie Jia Wei-ping Zhang Ting-ting Fang Jie Hu Shan-Feng Ma Qin Gao |
| author_facet | Ying Yu Xian-Jie Jia Wei-ping Zhang Ting-ting Fang Jie Hu Shan-Feng Ma Qin Gao |
| author_sort | Ying Yu |
| collection | DOAJ |
| description | Objective. To investigate the effects of low dose ethanol feeding in diabetic rats and analyze its underlying mechanisms. Methods. Male Sprague-Dawley rats were divided into 4 groups: control (Con), diabetes at 4 weeks (DM4W), diabetes at 8 weeks (DM8W), and EtOH + DM8W. After 8 weeks, hemodynamic parameters were recorded and heart weight/body weight (H/B) and hydroxyproline (Hp) content in myocardium were measured. Morphology of collagen in myocardial tissue was observed with Masson’s trichrome staining method and collagen volume fraction (CVF) was analysed. The mRNA expression of ALDH2 was assessed with Real-Time PCR. The protein expressions of p-JNK and JNK were evaluated using western blot. Results. In contrast to Con group, there was no difference in hemodynamic parameters in DM4W group, but mean arterial pressure and heart rate were decreased in DM8W group, and the ratios of H/B, Hp, and CVF were markedly increased. ALDH2 mRNA expression was decreased, while the ratio of p-JNK/JNK were increased. Compared with DM8W group, the above indexes were improved in EtOH + DM8W group. Conclusion. With low dose ethanol intervention, enhanced ALDH2 expression can antagonize the happening of myocardial fibrosis in diabetic rats, which may be relevant with downregulating the JNK pathway. |
| format | Article |
| id | doaj-art-fddba461a86c42e489e99065b4562c05 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-fddba461a86c42e489e99065b4562c052025-02-03T01:31:50ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/38342833834283The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic RatsYing Yu0Xian-Jie Jia1Wei-ping Zhang2Ting-ting Fang3Jie Hu4Shan-Feng Ma5Qin Gao6Department of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, ChinaDepartment of Epidemiology and Statistics, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, ChinaDepartment of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, ChinaDepartment of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, ChinaDepartment of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, ChinaDepartment of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, ChinaDepartment of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, ChinaObjective. To investigate the effects of low dose ethanol feeding in diabetic rats and analyze its underlying mechanisms. Methods. Male Sprague-Dawley rats were divided into 4 groups: control (Con), diabetes at 4 weeks (DM4W), diabetes at 8 weeks (DM8W), and EtOH + DM8W. After 8 weeks, hemodynamic parameters were recorded and heart weight/body weight (H/B) and hydroxyproline (Hp) content in myocardium were measured. Morphology of collagen in myocardial tissue was observed with Masson’s trichrome staining method and collagen volume fraction (CVF) was analysed. The mRNA expression of ALDH2 was assessed with Real-Time PCR. The protein expressions of p-JNK and JNK were evaluated using western blot. Results. In contrast to Con group, there was no difference in hemodynamic parameters in DM4W group, but mean arterial pressure and heart rate were decreased in DM8W group, and the ratios of H/B, Hp, and CVF were markedly increased. ALDH2 mRNA expression was decreased, while the ratio of p-JNK/JNK were increased. Compared with DM8W group, the above indexes were improved in EtOH + DM8W group. Conclusion. With low dose ethanol intervention, enhanced ALDH2 expression can antagonize the happening of myocardial fibrosis in diabetic rats, which may be relevant with downregulating the JNK pathway.http://dx.doi.org/10.1155/2016/3834283 |
| spellingShingle | Ying Yu Xian-Jie Jia Wei-ping Zhang Ting-ting Fang Jie Hu Shan-Feng Ma Qin Gao The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats Journal of Diabetes Research |
| title | The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats |
| title_full | The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats |
| title_fullStr | The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats |
| title_full_unstemmed | The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats |
| title_short | The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats |
| title_sort | protective effect of low dose ethanol on myocardial fibrosis through downregulating the jnk signaling pathway in diabetic rats |
| url | http://dx.doi.org/10.1155/2016/3834283 |
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