Metabolomics of related C3 and C4 Flaveria species indicate differences in the operation of photorespiration under fluctuating light
Abstract C3 photosynthesis can be complemented with a C4 carbon concentrating mechanism (CCM) to minimize photorespiratory losses. C4 photosynthesis is often more efficient than C3 under steady‐state conditions. However, the C4 CCM depends on inter‐cellular metabolite concentration gradients, which...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-10-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.70012 |
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| _version_ | 1832541451103240192 |
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| author | Xinyu Fu Urte Schlüter Kaila Smith Andreas P. M. Weber Berkley J. Walker |
| author_facet | Xinyu Fu Urte Schlüter Kaila Smith Andreas P. M. Weber Berkley J. Walker |
| author_sort | Xinyu Fu |
| collection | DOAJ |
| description | Abstract C3 photosynthesis can be complemented with a C4 carbon concentrating mechanism (CCM) to minimize photorespiratory losses. C4 photosynthesis is often more efficient than C3 under steady‐state conditions. However, the C4 CCM depends on inter‐cellular metabolite concentration gradients, which must increase following increases in light intensity and could decrease rates of C4 photosynthesis under fluctuating light. Additionally, incomplete flux through photorespiration could prove beneficial to C4 assimilation during light induction of the CCM. Here, we compare metabolic profiles in the closely related C3 Flaveria robusta and C4 Flaveria bidentis during a light transient from low to high light to determine if these non‐steady state accumulation patterns provide insight to the induction of the metabolite gradients needed to drive C4 intermediate transport and if there is incomplete cycling of photorespiratory intermediates. In these C3 and C4 species, metabolite steady‐state pool sizes suggest that C4 transport acids maintain concentration gradients across the bundle sheath and mesophyll cell types under these light fluctuations. However, there was incomplete flux through photorespiration in the C4 F. bidentis, which could reduce photorespiratory CO2 loss via glycine decarboxylation and help maintain higher rates of assimilation during following induction periods. |
| format | Article |
| id | doaj-art-fdd3f2b8763a416ea8ebfa78caa1a522 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-fdd3f2b8763a416ea8ebfa78caa1a5222025-02-04T08:31:56ZengWileyPlant Direct2475-44552024-10-01810n/an/a10.1002/pld3.70012Metabolomics of related C3 and C4 Flaveria species indicate differences in the operation of photorespiration under fluctuating lightXinyu Fu0Urte Schlüter1Kaila Smith2Andreas P. M. Weber3Berkley J. Walker4Department of Energy‐Plant Research Laboratory Michigan State University East Lansing Michigan USAHeinrich‐Heine‐University Düsseldorf Düsseldorf GermanyDepartment of Energy‐Plant Research Laboratory Michigan State University East Lansing Michigan USAHeinrich‐Heine‐University Düsseldorf Düsseldorf GermanyDepartment of Energy‐Plant Research Laboratory Michigan State University East Lansing Michigan USAAbstract C3 photosynthesis can be complemented with a C4 carbon concentrating mechanism (CCM) to minimize photorespiratory losses. C4 photosynthesis is often more efficient than C3 under steady‐state conditions. However, the C4 CCM depends on inter‐cellular metabolite concentration gradients, which must increase following increases in light intensity and could decrease rates of C4 photosynthesis under fluctuating light. Additionally, incomplete flux through photorespiration could prove beneficial to C4 assimilation during light induction of the CCM. Here, we compare metabolic profiles in the closely related C3 Flaveria robusta and C4 Flaveria bidentis during a light transient from low to high light to determine if these non‐steady state accumulation patterns provide insight to the induction of the metabolite gradients needed to drive C4 intermediate transport and if there is incomplete cycling of photorespiratory intermediates. In these C3 and C4 species, metabolite steady‐state pool sizes suggest that C4 transport acids maintain concentration gradients across the bundle sheath and mesophyll cell types under these light fluctuations. However, there was incomplete flux through photorespiration in the C4 F. bidentis, which could reduce photorespiratory CO2 loss via glycine decarboxylation and help maintain higher rates of assimilation during following induction periods.https://doi.org/10.1002/pld3.70012C3 photosynthesisC4 photosynthesislight fluctuationsmetabolism |
| spellingShingle | Xinyu Fu Urte Schlüter Kaila Smith Andreas P. M. Weber Berkley J. Walker Metabolomics of related C3 and C4 Flaveria species indicate differences in the operation of photorespiration under fluctuating light Plant Direct C3 photosynthesis C4 photosynthesis light fluctuations metabolism |
| title | Metabolomics of related C3 and C4 Flaveria species indicate differences in the operation of photorespiration under fluctuating light |
| title_full | Metabolomics of related C3 and C4 Flaveria species indicate differences in the operation of photorespiration under fluctuating light |
| title_fullStr | Metabolomics of related C3 and C4 Flaveria species indicate differences in the operation of photorespiration under fluctuating light |
| title_full_unstemmed | Metabolomics of related C3 and C4 Flaveria species indicate differences in the operation of photorespiration under fluctuating light |
| title_short | Metabolomics of related C3 and C4 Flaveria species indicate differences in the operation of photorespiration under fluctuating light |
| title_sort | metabolomics of related c3 and c4 flaveria species indicate differences in the operation of photorespiration under fluctuating light |
| topic | C3 photosynthesis C4 photosynthesis light fluctuations metabolism |
| url | https://doi.org/10.1002/pld3.70012 |
| work_keys_str_mv | AT xinyufu metabolomicsofrelatedc3andc4flaveriaspeciesindicatedifferencesintheoperationofphotorespirationunderfluctuatinglight AT urteschluter metabolomicsofrelatedc3andc4flaveriaspeciesindicatedifferencesintheoperationofphotorespirationunderfluctuatinglight AT kailasmith metabolomicsofrelatedc3andc4flaveriaspeciesindicatedifferencesintheoperationofphotorespirationunderfluctuatinglight AT andreaspmweber metabolomicsofrelatedc3andc4flaveriaspeciesindicatedifferencesintheoperationofphotorespirationunderfluctuatinglight AT berkleyjwalker metabolomicsofrelatedc3andc4flaveriaspeciesindicatedifferencesintheoperationofphotorespirationunderfluctuatinglight |