Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential
Extensive use of pyrethroid insecticides poses significant risks to both ecological ecosystems and human beings. Herein, Pseudomonas aeruginosa PAO1 exhibited exceptional degradation capabilities towards a range of pyrethroid family insecticides including etofenprox, bifenthrin, tetramethrin, D-cype...
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Elsevier
2025-01-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412024008080 |
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| author | Hui Liu Wen-Juan Chen Zeling Xu Shao-Fang Chen Haoran Song Yaohua Huang Kalpana Bhatt Sandhya Mishra Mohamed A. Ghorab Lian-Hui Zhang Shaohua Chen |
| author_facet | Hui Liu Wen-Juan Chen Zeling Xu Shao-Fang Chen Haoran Song Yaohua Huang Kalpana Bhatt Sandhya Mishra Mohamed A. Ghorab Lian-Hui Zhang Shaohua Chen |
| author_sort | Hui Liu |
| collection | DOAJ |
| description | Extensive use of pyrethroid insecticides poses significant risks to both ecological ecosystems and human beings. Herein, Pseudomonas aeruginosa PAO1 exhibited exceptional degradation capabilities towards a range of pyrethroid family insecticides including etofenprox, bifenthrin, tetramethrin, D-cypermethrin, allethrin, and permethrin, with a degradation efficiency reaching over 84 % within 36 h (50 mg·L-1). Strain PAO1 demonstrated effective soil bioremediation by removing etofenprox across different concentrations (25–100 mg·kg−1), with a degradation efficiency over 77 % within 15 days. Additionally, 16S rDNA high-throughput sequencing analysis revealed that introduction of strain PAO1 and etofenprox had a notable impact on the soil microbial community. Strain PAO1 displayed a synergistic effect with local degrading bacteria or flora to degrade etofenprox. UPLC-MS/MS analysis identified 2-(4-ethoxyphenyl) propan-2-ol and 3-phenoxybenzoic acid as the major metabolites of etofenprox biodegradation. A new esterase gene (estA) containing conserved motif (GDSL) and catalytic triad (Ser38, Asp310 and His313) was cloned from strain PAO1. Enzyme activity and gene knockout experiments confirmed the pivotal role of estA in pyrethroid biodegradation. The findings from this study shed a new light on elucidating the degradation mechanism of P. aeruginosa PAO1 and present a useful agent for development of effective pyrethroid bioremediation strategies. |
| format | Article |
| id | doaj-art-fdcbd0bbaf654c0a92323568b7b1d872 |
| institution | Kabale University |
| issn | 0160-4120 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj-art-fdcbd0bbaf654c0a92323568b7b1d8722025-01-24T04:44:06ZengElsevierEnvironment International0160-41202025-01-01195109221Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potentialHui Liu0Wen-Juan Chen1Zeling Xu2Shao-Fang Chen3Haoran Song4Yaohua Huang5Kalpana Bhatt6Sandhya Mishra7Mohamed A. Ghorab8Lian-Hui Zhang9Shaohua Chen10State Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaEnvironmental Technologies Division, CSIR-National Botanical Research Institute (NBRI), Rana Pratap Marg, Lucknow 226001, IndiaWildlife Toxicology Lab, Department of Animal Science, Institute for Integrative Toxicology (IIT), Michigan State University, East Lansing, MI 48824, USA; Department of Molecular Biosciences, School of Veterinary Medicine, University of California Davis, Davis, CA 95616-8741, USAState Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, China; Corresponding authors.State Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, South China Agricultural University, Guangzhou 510642, China; Corresponding authors.Extensive use of pyrethroid insecticides poses significant risks to both ecological ecosystems and human beings. Herein, Pseudomonas aeruginosa PAO1 exhibited exceptional degradation capabilities towards a range of pyrethroid family insecticides including etofenprox, bifenthrin, tetramethrin, D-cypermethrin, allethrin, and permethrin, with a degradation efficiency reaching over 84 % within 36 h (50 mg·L-1). Strain PAO1 demonstrated effective soil bioremediation by removing etofenprox across different concentrations (25–100 mg·kg−1), with a degradation efficiency over 77 % within 15 days. Additionally, 16S rDNA high-throughput sequencing analysis revealed that introduction of strain PAO1 and etofenprox had a notable impact on the soil microbial community. Strain PAO1 displayed a synergistic effect with local degrading bacteria or flora to degrade etofenprox. UPLC-MS/MS analysis identified 2-(4-ethoxyphenyl) propan-2-ol and 3-phenoxybenzoic acid as the major metabolites of etofenprox biodegradation. A new esterase gene (estA) containing conserved motif (GDSL) and catalytic triad (Ser38, Asp310 and His313) was cloned from strain PAO1. Enzyme activity and gene knockout experiments confirmed the pivotal role of estA in pyrethroid biodegradation. The findings from this study shed a new light on elucidating the degradation mechanism of P. aeruginosa PAO1 and present a useful agent for development of effective pyrethroid bioremediation strategies.http://www.sciencedirect.com/science/article/pii/S0160412024008080BiodegradationPyrethroidsEtofenproxEsterasePseudomonas aeruginosa |
| spellingShingle | Hui Liu Wen-Juan Chen Zeling Xu Shao-Fang Chen Haoran Song Yaohua Huang Kalpana Bhatt Sandhya Mishra Mohamed A. Ghorab Lian-Hui Zhang Shaohua Chen Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential Environment International Biodegradation Pyrethroids Etofenprox Esterase Pseudomonas aeruginosa |
| title | Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential |
| title_full | Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential |
| title_fullStr | Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential |
| title_full_unstemmed | Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential |
| title_short | Unraveling the degradation mechanism of multiple pyrethroid insecticides by Pseudomonas aeruginosa and its environmental bioremediation potential |
| title_sort | unraveling the degradation mechanism of multiple pyrethroid insecticides by pseudomonas aeruginosa and its environmental bioremediation potential |
| topic | Biodegradation Pyrethroids Etofenprox Esterase Pseudomonas aeruginosa |
| url | http://www.sciencedirect.com/science/article/pii/S0160412024008080 |
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