Isolation and molecular characterization of New Delhi metallo-beta-lactamase-1 producing superbug in Bangladesh
Introduction: New Delhi metallo-beta-lactamse-1 (NDM-1) producing superbugs create a global public health problem because of their resistance to most antibiotics. This study was conducted to determine the presence of MBL producers, including NDM-1 producers, in Bangladesh, along with the antimicrob...
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The Journal of Infection in Developing Countries
2013-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/2493 |
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| author | Refath Farzana SM Shamsuzzaman Kazi Zulfiquer Mamun |
| author_facet | Refath Farzana SM Shamsuzzaman Kazi Zulfiquer Mamun |
| author_sort | Refath Farzana |
| collection | DOAJ |
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Introduction: New Delhi metallo-beta-lactamse-1 (NDM-1) producing superbugs create a global public health problem because of their resistance to most antibiotics. This study was conducted to determine the presence of MBL producers, including NDM-1 producers, in Bangladesh, along with the antimicrobial resistance patterns of these organisms.
Methodology: Thirty-five isolates resistant to imipenem by disk diffusion technique were investigated for MBL production. Minimum inhibitory concentration (MIC) of imipenem was determined by agar dilution method. MBL producers were phenotypically detected by double disk synergy test and combined disk assay. Gene encoding blaIMP-1, blaIMP-2, blaVIM-1, blaVIM-2, blaNDM-1 and class 1 integron was identified by PCR.
Results: Thirty-one (88.57%) MBL producers were detected by PCR, 24 (68.57%) by double disk synergy test, and 30 (85.71%) by combined disk assay. Eight (22.86%) were positive for blaNDM-1, 13 (37.15%) for blaVIM-1, 21 (60.00%) for blaVIM-2, 18 (51.43%) for blaIMP-1, and 9 (25.71%) for blaIMP-2. More than one blaMBL was present in 23 (65.71%) of the isolates. MIC of imipenem of MBL producers ranged from ≥256 µg/ml to ≤8 µg/ml. All the NDM-1 producing isolates carried class 1 integron. NDM-1 producers were 100% resistant to amoxicillin, cephradine, cefuroxime, ceftazidime, cefotaxime, ceftriaxone, aztreonam, gentamicin and piperacillin, 87.5% to amikacin, 75% to ciprofloxacin, and 62.5% to co-trimoxazole and the combination of tazobactam and piperacillin. All were sensitive to colistin.
Conclusion: The results of this study provide insight into the presence of blaMBL, including blaNDM-1, in Bangladesh. Urgent epidemiological monitoring of MBL producers in Bangladesh may combat their rapid dissemination.
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| format | Article |
| id | doaj-art-fdc656d2a5eb403ca0c4e4a4d3160825 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2013-03-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-fdc656d2a5eb403ca0c4e4a4d31608252025-08-20T02:14:15ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802013-03-0170310.3855/jidc.2493Isolation and molecular characterization of New Delhi metallo-beta-lactamase-1 producing superbug in BangladeshRefath Farzana0SM Shamsuzzaman1Kazi Zulfiquer Mamun2Dhaka Medical College, Department of Microbiology, Dhaka, BangladeshDhaka Medical College, Department of Microbiology, Dhaka, BangladeshDhaka Medical College, Department of Microbiology, Dhaka, Bangladesh Introduction: New Delhi metallo-beta-lactamse-1 (NDM-1) producing superbugs create a global public health problem because of their resistance to most antibiotics. This study was conducted to determine the presence of MBL producers, including NDM-1 producers, in Bangladesh, along with the antimicrobial resistance patterns of these organisms. Methodology: Thirty-five isolates resistant to imipenem by disk diffusion technique were investigated for MBL production. Minimum inhibitory concentration (MIC) of imipenem was determined by agar dilution method. MBL producers were phenotypically detected by double disk synergy test and combined disk assay. Gene encoding blaIMP-1, blaIMP-2, blaVIM-1, blaVIM-2, blaNDM-1 and class 1 integron was identified by PCR. Results: Thirty-one (88.57%) MBL producers were detected by PCR, 24 (68.57%) by double disk synergy test, and 30 (85.71%) by combined disk assay. Eight (22.86%) were positive for blaNDM-1, 13 (37.15%) for blaVIM-1, 21 (60.00%) for blaVIM-2, 18 (51.43%) for blaIMP-1, and 9 (25.71%) for blaIMP-2. More than one blaMBL was present in 23 (65.71%) of the isolates. MIC of imipenem of MBL producers ranged from ≥256 µg/ml to ≤8 µg/ml. All the NDM-1 producing isolates carried class 1 integron. NDM-1 producers were 100% resistant to amoxicillin, cephradine, cefuroxime, ceftazidime, cefotaxime, ceftriaxone, aztreonam, gentamicin and piperacillin, 87.5% to amikacin, 75% to ciprofloxacin, and 62.5% to co-trimoxazole and the combination of tazobactam and piperacillin. All were sensitive to colistin. Conclusion: The results of this study provide insight into the presence of blaMBL, including blaNDM-1, in Bangladesh. Urgent epidemiological monitoring of MBL producers in Bangladesh may combat their rapid dissemination. https://jidc.org/index.php/journal/article/view/2493metallo-beta-lactamaseNew Delhi metallo-beta-lactamase-1Bangladeshmolecular characterizationantimicrobial susceptibility patterns |
| spellingShingle | Refath Farzana SM Shamsuzzaman Kazi Zulfiquer Mamun Isolation and molecular characterization of New Delhi metallo-beta-lactamase-1 producing superbug in Bangladesh Journal of Infection in Developing Countries metallo-beta-lactamase New Delhi metallo-beta-lactamase-1 Bangladesh molecular characterization antimicrobial susceptibility patterns |
| title | Isolation and molecular characterization of New Delhi metallo-beta-lactamase-1 producing superbug in Bangladesh |
| title_full | Isolation and molecular characterization of New Delhi metallo-beta-lactamase-1 producing superbug in Bangladesh |
| title_fullStr | Isolation and molecular characterization of New Delhi metallo-beta-lactamase-1 producing superbug in Bangladesh |
| title_full_unstemmed | Isolation and molecular characterization of New Delhi metallo-beta-lactamase-1 producing superbug in Bangladesh |
| title_short | Isolation and molecular characterization of New Delhi metallo-beta-lactamase-1 producing superbug in Bangladesh |
| title_sort | isolation and molecular characterization of new delhi metallo beta lactamase 1 producing superbug in bangladesh |
| topic | metallo-beta-lactamase New Delhi metallo-beta-lactamase-1 Bangladesh molecular characterization antimicrobial susceptibility patterns |
| url | https://jidc.org/index.php/journal/article/view/2493 |
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