Beneficial effects of AOS-iron supplementation on intestinal structure and microbiota in IDA rats
The objective of this study was to investigate the effects of agar oligosaccharide-iron (AOS-iron) on intestinal tissue pathology and microbiota in IDA rats induced by a low-iron diet, further to find the relationship between intestinal microbiota and iron metabolic disorders. After 4 weeks of AOS-i...
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Tsinghua University Press
2021-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302030152X |
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| author | Hong He Hui Teng Qun Huang Dan He Fengping An Lei Chen Hongbo Song |
| author_facet | Hong He Hui Teng Qun Huang Dan He Fengping An Lei Chen Hongbo Song |
| author_sort | Hong He |
| collection | DOAJ |
| description | The objective of this study was to investigate the effects of agar oligosaccharide-iron (AOS-iron) on intestinal tissue pathology and microbiota in IDA rats induced by a low-iron diet, further to find the relationship between intestinal microbiota and iron metabolic disorders. After 4 weeks of AOS-iron supplementation, the fecal iron content of IDA rats markedly increased in a dose-dependent manner, only the damaged cecum and colon tissues in medium-dose (MD) and high-dose (HD) groups were repaired to the baseline, while the diversity of gut microbiota was improved even at low dose (LD). Furthermore, the supplementation of AOS-iron altered the composition of gut microbiota. At the genus level, the beneficial microbiota was enriched in AOS-iron groups, but the relative abundance of potential opportunistic pathogens obviously reduced compared to that in the anemia model (AM) group. Spearman’s correlation analysis revealed that biochemical parameters, including blood metabolic parameters, iron contents, body weight, GSH-PX and T-AOC activity, were positively correlated with SMB53, Anaerotruncus, Anaerostipes and Coprobacillus but negatively correlated with Morganella, Fusobacterium and Serratia. These findings indicated that AOS-iron effectively repaired the damaged intestinal tissue and ameliorated iron metabolic disorders by regulating gut microbiota desirably, which could provide references for the treatment of IDA. |
| format | Article |
| id | doaj-art-fd9708eb3e9647ad9539122bbb502ca2 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fd9708eb3e9647ad9539122bbb502ca22025-02-03T06:51:28ZengTsinghua University PressFood Science and Human Wellness2213-45302021-01-011012331Beneficial effects of AOS-iron supplementation on intestinal structure and microbiota in IDA ratsHong He0Hui Teng1Qun Huang2Dan He3Fengping An4Lei Chen5Hongbo Song6College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Corresponding authors.College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fuzhou 350002, China; Corresponding authors.The objective of this study was to investigate the effects of agar oligosaccharide-iron (AOS-iron) on intestinal tissue pathology and microbiota in IDA rats induced by a low-iron diet, further to find the relationship between intestinal microbiota and iron metabolic disorders. After 4 weeks of AOS-iron supplementation, the fecal iron content of IDA rats markedly increased in a dose-dependent manner, only the damaged cecum and colon tissues in medium-dose (MD) and high-dose (HD) groups were repaired to the baseline, while the diversity of gut microbiota was improved even at low dose (LD). Furthermore, the supplementation of AOS-iron altered the composition of gut microbiota. At the genus level, the beneficial microbiota was enriched in AOS-iron groups, but the relative abundance of potential opportunistic pathogens obviously reduced compared to that in the anemia model (AM) group. Spearman’s correlation analysis revealed that biochemical parameters, including blood metabolic parameters, iron contents, body weight, GSH-PX and T-AOC activity, were positively correlated with SMB53, Anaerotruncus, Anaerostipes and Coprobacillus but negatively correlated with Morganella, Fusobacterium and Serratia. These findings indicated that AOS-iron effectively repaired the damaged intestinal tissue and ameliorated iron metabolic disorders by regulating gut microbiota desirably, which could provide references for the treatment of IDA.http://www.sciencedirect.com/science/article/pii/S221345302030152XAOS-ironIron deficiency anemiaRatsIntestinal tissue pathologyGut microbiota |
| spellingShingle | Hong He Hui Teng Qun Huang Dan He Fengping An Lei Chen Hongbo Song Beneficial effects of AOS-iron supplementation on intestinal structure and microbiota in IDA rats Food Science and Human Wellness AOS-iron Iron deficiency anemia Rats Intestinal tissue pathology Gut microbiota |
| title | Beneficial effects of AOS-iron supplementation on intestinal structure and microbiota in IDA rats |
| title_full | Beneficial effects of AOS-iron supplementation on intestinal structure and microbiota in IDA rats |
| title_fullStr | Beneficial effects of AOS-iron supplementation on intestinal structure and microbiota in IDA rats |
| title_full_unstemmed | Beneficial effects of AOS-iron supplementation on intestinal structure and microbiota in IDA rats |
| title_short | Beneficial effects of AOS-iron supplementation on intestinal structure and microbiota in IDA rats |
| title_sort | beneficial effects of aos iron supplementation on intestinal structure and microbiota in ida rats |
| topic | AOS-iron Iron deficiency anemia Rats Intestinal tissue pathology Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S221345302030152X |
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