Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver Transplantation
Recent investigations have indicated that reactive metabolites and AGE-RAGE-mediated inflammation might play an important role in the pathogenesis of ischemia-reperfusion injury in liver transplantation. In this observational clinical study, 150 patients were enrolled following liver transplantation...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/501430 |
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| author | Thorsten Brenner Thomas H. Fleming David Spranz Peter Schemmer Thomas Bruckner Florian Uhle Eike O. Martin Markus A. Weigand Stefan Hofer |
| author_facet | Thorsten Brenner Thomas H. Fleming David Spranz Peter Schemmer Thomas Bruckner Florian Uhle Eike O. Martin Markus A. Weigand Stefan Hofer |
| author_sort | Thorsten Brenner |
| collection | DOAJ |
| description | Recent investigations have indicated that reactive metabolites and AGE-RAGE-mediated inflammation might play an important role in the pathogenesis of ischemia-reperfusion injury in liver transplantation. In this observational clinical study, 150 patients were enrolled following liver transplantation from deceased donors. The occurrence of short-term complications within 10 days of transplantation was documented. Blood samples were collected prior to transplantation, immediately after transplantation, and at consecutive time points, for a total of seven days after transplantation. Plasma levels of methylglyoxal were determined using HPLC, whereas plasma levels of L-arginine, asymmetric dimethylarginine, advanced glycation endproducts-carboxylmethyllysine, soluble receptor for advanced glycation endproducts, and total antioxidant capacity were measured by ELISA. Patients following liver transplantation were shown to suffer from increased RAGE-associated inflammation with an AGE load mainly dependent upon reactive carbonyl species-derived AGEs. In contrast, carboxylmethyllysine-derived AGEs were of a minor importance. As assessed by the ratio of L-arginine/asymmetric dimethylarginine, the bioavailability of nitric oxide was shown to be reduced in hepatic IRI, especially in those patients suffering from perfusion disorders following liver transplantation. For the early identification of patients at high risk of perfusion disorders, the implementation of asymmetric dimethylarginine measurements in routine diagnostics following liver transplantation from deceased donors should be taken into consideration. |
| format | Article |
| id | doaj-art-fd96454478a9484a9427aa4ae19deae1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-fd96454478a9484a9427aa4ae19deae12025-02-03T01:01:58ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/501430501430Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver TransplantationThorsten Brenner0Thomas H. Fleming1David Spranz2Peter Schemmer3Thomas Bruckner4Florian Uhle5Eike O. Martin6Markus A. Weigand7Stefan Hofer8Department of Anesthesiology, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, GermanyDepartment of Medicine I and Clinical Chemistry, University of Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, GermanyDepartment of Anesthesiology, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, GermanyDepartment of General and Transplant Surgery, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, GermanyInstitute of Medical Biometry and Informatics, University of Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, GermanyDepartment of Anesthesiology and Intensive Care Medicine, University of Gießen, Rudolf-Buchheim-Straße 7, 35392 Gießen, GermanyDepartment of Anesthesiology, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, GermanyDepartment of Anesthesiology and Intensive Care Medicine, University of Gießen, Rudolf-Buchheim-Straße 7, 35392 Gießen, GermanyDepartment of Anesthesiology, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, GermanyRecent investigations have indicated that reactive metabolites and AGE-RAGE-mediated inflammation might play an important role in the pathogenesis of ischemia-reperfusion injury in liver transplantation. In this observational clinical study, 150 patients were enrolled following liver transplantation from deceased donors. The occurrence of short-term complications within 10 days of transplantation was documented. Blood samples were collected prior to transplantation, immediately after transplantation, and at consecutive time points, for a total of seven days after transplantation. Plasma levels of methylglyoxal were determined using HPLC, whereas plasma levels of L-arginine, asymmetric dimethylarginine, advanced glycation endproducts-carboxylmethyllysine, soluble receptor for advanced glycation endproducts, and total antioxidant capacity were measured by ELISA. Patients following liver transplantation were shown to suffer from increased RAGE-associated inflammation with an AGE load mainly dependent upon reactive carbonyl species-derived AGEs. In contrast, carboxylmethyllysine-derived AGEs were of a minor importance. As assessed by the ratio of L-arginine/asymmetric dimethylarginine, the bioavailability of nitric oxide was shown to be reduced in hepatic IRI, especially in those patients suffering from perfusion disorders following liver transplantation. For the early identification of patients at high risk of perfusion disorders, the implementation of asymmetric dimethylarginine measurements in routine diagnostics following liver transplantation from deceased donors should be taken into consideration.http://dx.doi.org/10.1155/2013/501430 |
| spellingShingle | Thorsten Brenner Thomas H. Fleming David Spranz Peter Schemmer Thomas Bruckner Florian Uhle Eike O. Martin Markus A. Weigand Stefan Hofer Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver Transplantation Mediators of Inflammation |
| title | Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver Transplantation |
| title_full | Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver Transplantation |
| title_fullStr | Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver Transplantation |
| title_full_unstemmed | Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver Transplantation |
| title_short | Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver Transplantation |
| title_sort | reactive metabolites and age rage mediated inflammation in patients following liver transplantation |
| url | http://dx.doi.org/10.1155/2013/501430 |
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