Anthocyanin Colorimetric Strip for Volatile Amine Determination

Food freshness is one of the main concerns of consumers. Food spoilage is mainly caused by contamination and microbial growth in which the latter produces volatile amines in the process. Several methods have been used to determine volatile amines to indicate food freshness, and indicator films are d...

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Main Authors: Ricarl Irish F. Agunos, Danilet Vi M. Mendoza, Michael Angelo S. Rivera
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:International Journal of Food Science
Online Access:http://dx.doi.org/10.1155/2020/1672851
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author Ricarl Irish F. Agunos
Danilet Vi M. Mendoza
Michael Angelo S. Rivera
author_facet Ricarl Irish F. Agunos
Danilet Vi M. Mendoza
Michael Angelo S. Rivera
author_sort Ricarl Irish F. Agunos
collection DOAJ
description Food freshness is one of the main concerns of consumers. Food spoilage is mainly caused by contamination and microbial growth in which the latter produces volatile amines in the process. Several methods have been used to determine volatile amines to indicate food freshness, and indicator films are deemed as the most time-efficient and economical. In this study, anthocyanin was extracted from mangosteen rind as a natural dye indicator and was incorporated in a chitosan/PVA polymer matrix. The film with different concentrations of anthocyanin extract (5%, 15%, and 25%) was prepared and tested for their sensitivity to 136 ppm ammonia vapor followed by colorimetric analysis using ImageJ software. The film with 25% anthocyanin yielded the most visible color change upon exposure to ammonia vapor. The color changed from pink to yellowish-brown within 14 minutes of exposure. The RGB-converted images of the film with 25% anthocyanin extract showed gradual loss of red coloration being replaced by cyan spots. FTIR spectra showed incorporation of anthocyanin to the chitosan/PVA matrix with the decrease in the intensity of the C-N stretching peak. Thermogravimetric analysis showed that the film has high thermal stability with onset temperature of 310.43°C. Thus, the film developed is an excellent candidate for optimization and production of a thermally stable amine detector for food products.
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series International Journal of Food Science
spelling doaj-art-fd750ac224f64445954565172b020c0d2025-02-03T06:46:41ZengWileyInternational Journal of Food Science2356-70152314-57652020-01-01202010.1155/2020/16728511672851Anthocyanin Colorimetric Strip for Volatile Amine DeterminationRicarl Irish F. Agunos0Danilet Vi M. Mendoza1Michael Angelo S. Rivera2Chemistry and Environmental Science Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology, Cabanatuan City 3100, PhilippinesChemistry and Environmental Science Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology, Cabanatuan City 3100, PhilippinesChemistry and Environmental Science Department, College of Arts and Sciences, Nueva Ecija University of Science and Technology, Cabanatuan City 3100, PhilippinesFood freshness is one of the main concerns of consumers. Food spoilage is mainly caused by contamination and microbial growth in which the latter produces volatile amines in the process. Several methods have been used to determine volatile amines to indicate food freshness, and indicator films are deemed as the most time-efficient and economical. In this study, anthocyanin was extracted from mangosteen rind as a natural dye indicator and was incorporated in a chitosan/PVA polymer matrix. The film with different concentrations of anthocyanin extract (5%, 15%, and 25%) was prepared and tested for their sensitivity to 136 ppm ammonia vapor followed by colorimetric analysis using ImageJ software. The film with 25% anthocyanin yielded the most visible color change upon exposure to ammonia vapor. The color changed from pink to yellowish-brown within 14 minutes of exposure. The RGB-converted images of the film with 25% anthocyanin extract showed gradual loss of red coloration being replaced by cyan spots. FTIR spectra showed incorporation of anthocyanin to the chitosan/PVA matrix with the decrease in the intensity of the C-N stretching peak. Thermogravimetric analysis showed that the film has high thermal stability with onset temperature of 310.43°C. Thus, the film developed is an excellent candidate for optimization and production of a thermally stable amine detector for food products.http://dx.doi.org/10.1155/2020/1672851
spellingShingle Ricarl Irish F. Agunos
Danilet Vi M. Mendoza
Michael Angelo S. Rivera
Anthocyanin Colorimetric Strip for Volatile Amine Determination
International Journal of Food Science
title Anthocyanin Colorimetric Strip for Volatile Amine Determination
title_full Anthocyanin Colorimetric Strip for Volatile Amine Determination
title_fullStr Anthocyanin Colorimetric Strip for Volatile Amine Determination
title_full_unstemmed Anthocyanin Colorimetric Strip for Volatile Amine Determination
title_short Anthocyanin Colorimetric Strip for Volatile Amine Determination
title_sort anthocyanin colorimetric strip for volatile amine determination
url http://dx.doi.org/10.1155/2020/1672851
work_keys_str_mv AT ricarlirishfagunos anthocyanincolorimetricstripforvolatileaminedetermination
AT daniletvimmendoza anthocyanincolorimetricstripforvolatileaminedetermination
AT michaelangelosrivera anthocyanincolorimetricstripforvolatileaminedetermination