Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolates
Abstracts Background: Proteus mirabilis are the most prevalent uropathogens associated with urinary tract infections (UTIs). Infection occurs only when the pathogenicity of the bacteria surpasses the host’s regular defensive systems. A lifetime incidence of between 50% and 60% among adult women, inc...
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Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Medical Journal of Babylon |
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| Online Access: | https://doi.org/10.4103/MJBL.MJBL_494_23 |
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| author | Kadhim Jwad Jamin Hussein O. M. Al-Dahmosi Sura Ihsan Abed Jabuk |
| author_facet | Kadhim Jwad Jamin Hussein O. M. Al-Dahmosi Sura Ihsan Abed Jabuk |
| author_sort | Kadhim Jwad Jamin |
| collection | DOAJ |
| description | Abstracts Background: Proteus mirabilis are the most prevalent uropathogens associated with urinary tract infections (UTIs). Infection occurs only when the pathogenicity of the bacteria surpasses the host’s regular defensive systems. A lifetime incidence of between 50% and 60% among adult women, increasing with age. Materials and Methods: Collected 450 (325 male and 125 female patients) with UTIs. These patients ranged in age from (17 to 70) years from four Iraqi hospitals (Merjan Hospital, Shomali General Hospital, Al-Hilla Surgical Teaching Hospital, and Babylon Hospital for Maternal and Pediatrics) from the period from July 2021 to the end of October 2022. The identification of bacterial isolates were carried out using standard bacteriological procedures. The antibiotics susceptibility test was carried out by using 12 antibiotics from different classes The molecular identification was done by using polymerase chain reaction amplification of the three bla CTX-M gene (bla CTX-MI, bla CTX-MIV, bla CTX-MII(. Results: The percentage of samples positive for P. mirabilis based on culture, biochemical, and Gram stain results was 70 (15.5%) of the total 450 samples. The percentage of P. mirabilis isolated from male patients was 21 (30%), whereas the percentage of P. mirabilis isolated from female patients was 49 (70%) of the total 70 isolates. The result of antibiotic susceptibility showed the high resistance against nitrofurantoin (92.8%), while the higher resistance was against amikacin (10%), imipenem (4.2%), aztreonam (2.8%), and meropenem (1.4%). The result of the current study showed the percentage of bacterial sample positive to the bla CTX-MI, bla CTX-MII, bla CTX-MIV was 9 (12.85), 56 (80), and 40 (57), respectively, among 70 bacteria isolates. Conclusions: The result of the study showed the prevalence of antibiotic resistant gene and multidrug resistant among P. mirabilis isolated from UTIs. |
| format | Article |
| id | doaj-art-fd73be7beaa640b39eeaed72155a9699 |
| institution | Kabale University |
| issn | 1812-156X 2312-6760 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Babylon |
| spelling | doaj-art-fd73be7beaa640b39eeaed72155a96992025-01-25T10:14:50ZengWolters Kluwer Medknow PublicationsMedical Journal of Babylon1812-156X2312-67602024-01-0121113714310.4103/MJBL.MJBL_494_23Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolatesKadhim Jwad JaminHussein O. M. Al-DahmosiSura Ihsan Abed JabukAbstracts Background: Proteus mirabilis are the most prevalent uropathogens associated with urinary tract infections (UTIs). Infection occurs only when the pathogenicity of the bacteria surpasses the host’s regular defensive systems. A lifetime incidence of between 50% and 60% among adult women, increasing with age. Materials and Methods: Collected 450 (325 male and 125 female patients) with UTIs. These patients ranged in age from (17 to 70) years from four Iraqi hospitals (Merjan Hospital, Shomali General Hospital, Al-Hilla Surgical Teaching Hospital, and Babylon Hospital for Maternal and Pediatrics) from the period from July 2021 to the end of October 2022. The identification of bacterial isolates were carried out using standard bacteriological procedures. The antibiotics susceptibility test was carried out by using 12 antibiotics from different classes The molecular identification was done by using polymerase chain reaction amplification of the three bla CTX-M gene (bla CTX-MI, bla CTX-MIV, bla CTX-MII(. Results: The percentage of samples positive for P. mirabilis based on culture, biochemical, and Gram stain results was 70 (15.5%) of the total 450 samples. The percentage of P. mirabilis isolated from male patients was 21 (30%), whereas the percentage of P. mirabilis isolated from female patients was 49 (70%) of the total 70 isolates. The result of antibiotic susceptibility showed the high resistance against nitrofurantoin (92.8%), while the higher resistance was against amikacin (10%), imipenem (4.2%), aztreonam (2.8%), and meropenem (1.4%). The result of the current study showed the percentage of bacterial sample positive to the bla CTX-MI, bla CTX-MII, bla CTX-MIV was 9 (12.85), 56 (80), and 40 (57), respectively, among 70 bacteria isolates. Conclusions: The result of the study showed the prevalence of antibiotic resistant gene and multidrug resistant among P. mirabilis isolated from UTIs.https://doi.org/10.4103/MJBL.MJBL_494_23antibiotics susceptibilityproteus mirabilisurinary tract infections |
| spellingShingle | Kadhim Jwad Jamin Hussein O. M. Al-Dahmosi Sura Ihsan Abed Jabuk Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolates Medical Journal of Babylon antibiotics susceptibility proteus mirabilis urinary tract infections |
| title | Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolates |
| title_full | Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolates |
| title_fullStr | Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolates |
| title_full_unstemmed | Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolates |
| title_short | Molecular study of blaCTX-M profile among uropathogenic Proteus mirabilis isolates |
| title_sort | molecular study of blactx m profile among uropathogenic proteus mirabilis isolates |
| topic | antibiotics susceptibility proteus mirabilis urinary tract infections |
| url | https://doi.org/10.4103/MJBL.MJBL_494_23 |
| work_keys_str_mv | AT kadhimjwadjamin molecularstudyofblactxmprofileamonguropathogenicproteusmirabilisisolates AT husseinomaldahmosi molecularstudyofblactxmprofileamonguropathogenicproteusmirabilisisolates AT suraihsanabedjabuk molecularstudyofblactxmprofileamonguropathogenicproteusmirabilisisolates |