Diversity and Plant Growth-Promoting Properties of <i>Rhodiola rosea</i> Root Endophytic Bacteria
Plants inhabiting environments with suboptimal growth conditions often have a more pronounced capacity to attract and sustain microbial communities that improve nutrient absorption and expand abiotic stress tolerance. <i>Rhodiola rosea</i> L. is a succulent plant of the <i>Crassula...
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2024-12-01
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| author | Inga Tamošiūnė Muhammad Fahad Hakim Odeta Buzaitė Vidmantas Stanys Jurgita Vinskienė Elena Andriūnaitė Danas Baniulis |
| author_facet | Inga Tamošiūnė Muhammad Fahad Hakim Odeta Buzaitė Vidmantas Stanys Jurgita Vinskienė Elena Andriūnaitė Danas Baniulis |
| author_sort | Inga Tamošiūnė |
| collection | DOAJ |
| description | Plants inhabiting environments with suboptimal growth conditions often have a more pronounced capacity to attract and sustain microbial communities that improve nutrient absorption and expand abiotic stress tolerance. <i>Rhodiola rosea</i> L. is a succulent plant of the <i>Crassulaceae</i> family adapted to survive in sandy or rocky soils or dry tundra. The aim of the present study was to investigate the diversity and plant growth-stimulating potential of <i>R. rosea</i> endophytic microbiota. Metataxonomic analysis of the bacterial diversity in the rhizome of <i>R. rosea</i> revealed 108 families. Among these, three families were found exclusively in the core microbiome of 1-year-old plants, while nine families were unique to the core microbiome of mature plants grown in the field for more than 4 years. Seventy-three endophytic bacteria isolates were obtained from the rhizome of <i>R. rosea</i> plants and were assigned into 14 distinct bacterial genera of Firmicutes (26%) or Proteobacteria (74%) phyla. Screening for functional genes related to the nitrogen cycle, phosphorus mineralisation or dissolution, and traits associated with nitrogen fixation (56% of isolates), siderophore production (40%), inorganic phosphorus solubilisation (30%), and production of indole-related compounds (51%) led to the classification of the isolates into 16 distinct clusters. Co-cultivation of 45 selected isolates with germinating Arabidopsis seedlings revealed 18 and 5 isolates that resulted in more than a 20% increase in root or shoot growth, respectively. The study results established the complexity of the succulent <i>R. rosea</i> endophytic microbiome and identified isolates for potential plant growth-stimulating applications. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-fd5b52e587e949938d89a646a7bca4c12025-01-24T13:42:17ZengMDPI AGMicroorganisms2076-26072024-12-011311310.3390/microorganisms13010013Diversity and Plant Growth-Promoting Properties of <i>Rhodiola rosea</i> Root Endophytic BacteriaInga Tamošiūnė0Muhammad Fahad Hakim1Odeta Buzaitė2Vidmantas Stanys3Jurgita Vinskienė4Elena Andriūnaitė5Danas Baniulis6Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, 54333 Babtai, Kaunas reg., LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, 54333 Babtai, Kaunas reg., LithuaniaDepartment of Biochemistry, Vytautas Magnus University, Universiteto Str. 10, 53361 Akademija, Kaunas reg., LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, 54333 Babtai, Kaunas reg., LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, 54333 Babtai, Kaunas reg., LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, 54333 Babtai, Kaunas reg., LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, 54333 Babtai, Kaunas reg., LithuaniaPlants inhabiting environments with suboptimal growth conditions often have a more pronounced capacity to attract and sustain microbial communities that improve nutrient absorption and expand abiotic stress tolerance. <i>Rhodiola rosea</i> L. is a succulent plant of the <i>Crassulaceae</i> family adapted to survive in sandy or rocky soils or dry tundra. The aim of the present study was to investigate the diversity and plant growth-stimulating potential of <i>R. rosea</i> endophytic microbiota. Metataxonomic analysis of the bacterial diversity in the rhizome of <i>R. rosea</i> revealed 108 families. Among these, three families were found exclusively in the core microbiome of 1-year-old plants, while nine families were unique to the core microbiome of mature plants grown in the field for more than 4 years. Seventy-three endophytic bacteria isolates were obtained from the rhizome of <i>R. rosea</i> plants and were assigned into 14 distinct bacterial genera of Firmicutes (26%) or Proteobacteria (74%) phyla. Screening for functional genes related to the nitrogen cycle, phosphorus mineralisation or dissolution, and traits associated with nitrogen fixation (56% of isolates), siderophore production (40%), inorganic phosphorus solubilisation (30%), and production of indole-related compounds (51%) led to the classification of the isolates into 16 distinct clusters. Co-cultivation of 45 selected isolates with germinating Arabidopsis seedlings revealed 18 and 5 isolates that resulted in more than a 20% increase in root or shoot growth, respectively. The study results established the complexity of the succulent <i>R. rosea</i> endophytic microbiome and identified isolates for potential plant growth-stimulating applications.https://www.mdpi.com/2076-2607/13/1/13metataxonomic analysismicrobiomeplant-associated bacteriarhizomesucculent plant |
| spellingShingle | Inga Tamošiūnė Muhammad Fahad Hakim Odeta Buzaitė Vidmantas Stanys Jurgita Vinskienė Elena Andriūnaitė Danas Baniulis Diversity and Plant Growth-Promoting Properties of <i>Rhodiola rosea</i> Root Endophytic Bacteria Microorganisms metataxonomic analysis microbiome plant-associated bacteria rhizome succulent plant |
| title | Diversity and Plant Growth-Promoting Properties of <i>Rhodiola rosea</i> Root Endophytic Bacteria |
| title_full | Diversity and Plant Growth-Promoting Properties of <i>Rhodiola rosea</i> Root Endophytic Bacteria |
| title_fullStr | Diversity and Plant Growth-Promoting Properties of <i>Rhodiola rosea</i> Root Endophytic Bacteria |
| title_full_unstemmed | Diversity and Plant Growth-Promoting Properties of <i>Rhodiola rosea</i> Root Endophytic Bacteria |
| title_short | Diversity and Plant Growth-Promoting Properties of <i>Rhodiola rosea</i> Root Endophytic Bacteria |
| title_sort | diversity and plant growth promoting properties of i rhodiola rosea i root endophytic bacteria |
| topic | metataxonomic analysis microbiome plant-associated bacteria rhizome succulent plant |
| url | https://www.mdpi.com/2076-2607/13/1/13 |
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