An Enhanced GC/MS Procedure for the Identification of Proteins in Paint Microsamples
The chemical characterization of materials used in works of art is extremely useful for gaining a better knowledge of the artistic heritage and to guarantee its preservation. A derivatization GC/MS procedure for the identification of proteins in a microsample from painted works of art has been optim...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/6032084 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556170128130048 |
|---|---|
| author | D. Fico E. Margapoti A. Pennetta G. E. De Benedetto |
| author_facet | D. Fico E. Margapoti A. Pennetta G. E. De Benedetto |
| author_sort | D. Fico |
| collection | DOAJ |
| description | The chemical characterization of materials used in works of art is extremely useful for gaining a better knowledge of the artistic heritage and to guarantee its preservation. A derivatization GC/MS procedure for the identification of proteins in a microsample from painted works of art has been optimized. The amino acid fraction is derivatized using anhydrous dimethylformamide (DMF) as solvent instead of pyridine (Py), commonly used to facilitate the reaction. Although pyridine is often considered a silylation catalyst, there are many instances in which silylation reactions actually are slower in pyridine than other solvents. In addition, pyridine also may have other undesirable effects such as the promotion of secondary products and other chromatographic anomalies. Using DMF, the formation of artifacts is limited and the derivatization yield of hydrophilic amino acids such as proline and hydroxyproline has improved, thus making the identification of organic paint media more straightforward. The method has been validated and successfully applied to identify the binder of the sample taken from the pictorial cycle of the 12th century monastery of Santa Maria delle Cerrate (Lecce, Italy), thus highlighting the use of eggs as a binding medium. |
| format | Article |
| id | doaj-art-fd39049885b446cbb42d02da2ac9843a |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-fd39049885b446cbb42d02da2ac9843a2025-02-03T05:46:13ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/60320846032084An Enhanced GC/MS Procedure for the Identification of Proteins in Paint MicrosamplesD. Fico0E. Margapoti1A. Pennetta2G. E. De Benedetto3Laboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, 73100 Lecce, ItalyIBAM-CNR, Sede di Lecce, Via per Monteroni, 73100 Lecce, ItalyLaboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, 73100 Lecce, ItalyLaboratorio di Spettrometria di Massa Analitica ed Isotopica, Dipartimento di Beni Culturali, Università del Salento, 73100 Lecce, ItalyThe chemical characterization of materials used in works of art is extremely useful for gaining a better knowledge of the artistic heritage and to guarantee its preservation. A derivatization GC/MS procedure for the identification of proteins in a microsample from painted works of art has been optimized. The amino acid fraction is derivatized using anhydrous dimethylformamide (DMF) as solvent instead of pyridine (Py), commonly used to facilitate the reaction. Although pyridine is often considered a silylation catalyst, there are many instances in which silylation reactions actually are slower in pyridine than other solvents. In addition, pyridine also may have other undesirable effects such as the promotion of secondary products and other chromatographic anomalies. Using DMF, the formation of artifacts is limited and the derivatization yield of hydrophilic amino acids such as proline and hydroxyproline has improved, thus making the identification of organic paint media more straightforward. The method has been validated and successfully applied to identify the binder of the sample taken from the pictorial cycle of the 12th century monastery of Santa Maria delle Cerrate (Lecce, Italy), thus highlighting the use of eggs as a binding medium.http://dx.doi.org/10.1155/2018/6032084 |
| spellingShingle | D. Fico E. Margapoti A. Pennetta G. E. De Benedetto An Enhanced GC/MS Procedure for the Identification of Proteins in Paint Microsamples Journal of Analytical Methods in Chemistry |
| title | An Enhanced GC/MS Procedure for the Identification of Proteins in Paint Microsamples |
| title_full | An Enhanced GC/MS Procedure for the Identification of Proteins in Paint Microsamples |
| title_fullStr | An Enhanced GC/MS Procedure for the Identification of Proteins in Paint Microsamples |
| title_full_unstemmed | An Enhanced GC/MS Procedure for the Identification of Proteins in Paint Microsamples |
| title_short | An Enhanced GC/MS Procedure for the Identification of Proteins in Paint Microsamples |
| title_sort | enhanced gc ms procedure for the identification of proteins in paint microsamples |
| url | http://dx.doi.org/10.1155/2018/6032084 |
| work_keys_str_mv | AT dfico anenhancedgcmsprocedurefortheidentificationofproteinsinpaintmicrosamples AT emargapoti anenhancedgcmsprocedurefortheidentificationofproteinsinpaintmicrosamples AT apennetta anenhancedgcmsprocedurefortheidentificationofproteinsinpaintmicrosamples AT gedebenedetto anenhancedgcmsprocedurefortheidentificationofproteinsinpaintmicrosamples AT dfico enhancedgcmsprocedurefortheidentificationofproteinsinpaintmicrosamples AT emargapoti enhancedgcmsprocedurefortheidentificationofproteinsinpaintmicrosamples AT apennetta enhancedgcmsprocedurefortheidentificationofproteinsinpaintmicrosamples AT gedebenedetto enhancedgcmsprocedurefortheidentificationofproteinsinpaintmicrosamples |