Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran
This descriptive study was accomplished on 83 K. pneumoniae strains isolated from two hospitals in Tehran, Iran. Antibiotic susceptibility tests were performed by disc diffusion and broth microdilution methods. ESBLs, MBL, Amp-C, and KPC producing strains were detected by phenotypic confirmatory tes...
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Wiley
2014-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2014/726179 |
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| author | Ali Hashemi Fatemeh Fallah Soroor Erfanimanesh Parastu Hamedani Shadi Alimehr Hossein Goudarzi |
| author_facet | Ali Hashemi Fatemeh Fallah Soroor Erfanimanesh Parastu Hamedani Shadi Alimehr Hossein Goudarzi |
| author_sort | Ali Hashemi |
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| description | This descriptive study was accomplished on 83 K. pneumoniae strains isolated from two hospitals in Tehran, Iran. Antibiotic susceptibility tests were performed by disc diffusion and broth microdilution methods. ESBLs, MBL, Amp-C, and KPC producing strains were detected by phenotypic confirmatory test, combination disk diffusion test (CDDT), Amp-C detection kit, and modified Hodge test, respectively. OXA-48, NDM-1, and CTX-M-15 genes were detected by PCR and sequencing methods. The outer membrane porins such as OmpK35 and OmpK36 were analysed by SDS-PAGE, PCR, and sequencing methods. From 83 K. pneumoniae isolates, 48 (57.5%), 3 (3.5%), 23 (28%), and 5 (6%) were ESBL, MBL, Amp-C, and KPC positive, respectively. The CTX-M-15 gene was detected in 30 (62.5%) and OXA-48 gene was found in 2 (4.1%) of the 48 ESBL-producing isolates. Two isolates harboured both OXA-48 and CTX-M-15; NDM-1 gene was not detected in this study. Outer membrane porin, OmpK35, was detected in 30 (62.5%) of 48 ESBL-producing isolates while OmpK36 was found in 35 (72.91%) of 48 ESBL-producing isolates. In this study, fosfomycin and tigecycline were more effective than other antibiotics. The high prevalence of β-lactamase-producing K. pneumoniae detected in this study is of great concern, which requires infection control measures including antibacterial management and identification of β-lactamases-producing isolates. |
| format | Article |
| id | doaj-art-fd33b923a2d643c0ab4b216898c63215 |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-fd33b923a2d643c0ab4b216898c632152025-02-03T05:48:27ZengWileyScientifica2090-908X2014-01-01201410.1155/2014/726179726179Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in IranAli Hashemi0Fatemeh Fallah1Soroor Erfanimanesh2Parastu Hamedani3Shadi Alimehr4Hossein Goudarzi5Department of Microbiology, Shahid Beheshti University of Medical Sciences, P.O. Box 19857-17443, Tehran, IranPediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, Shahid Beheshti University of Medical Sciences, P.O. Box 19857-17443, Tehran, IranDepartment of Microbiology, Shahid Beheshti University of Medical Sciences, P.O. Box 19857-17443, Tehran, IranPediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, Shahid Beheshti University of Medical Sciences, P.O. Box 19857-17443, Tehran, IranThis descriptive study was accomplished on 83 K. pneumoniae strains isolated from two hospitals in Tehran, Iran. Antibiotic susceptibility tests were performed by disc diffusion and broth microdilution methods. ESBLs, MBL, Amp-C, and KPC producing strains were detected by phenotypic confirmatory test, combination disk diffusion test (CDDT), Amp-C detection kit, and modified Hodge test, respectively. OXA-48, NDM-1, and CTX-M-15 genes were detected by PCR and sequencing methods. The outer membrane porins such as OmpK35 and OmpK36 were analysed by SDS-PAGE, PCR, and sequencing methods. From 83 K. pneumoniae isolates, 48 (57.5%), 3 (3.5%), 23 (28%), and 5 (6%) were ESBL, MBL, Amp-C, and KPC positive, respectively. The CTX-M-15 gene was detected in 30 (62.5%) and OXA-48 gene was found in 2 (4.1%) of the 48 ESBL-producing isolates. Two isolates harboured both OXA-48 and CTX-M-15; NDM-1 gene was not detected in this study. Outer membrane porin, OmpK35, was detected in 30 (62.5%) of 48 ESBL-producing isolates while OmpK36 was found in 35 (72.91%) of 48 ESBL-producing isolates. In this study, fosfomycin and tigecycline were more effective than other antibiotics. The high prevalence of β-lactamase-producing K. pneumoniae detected in this study is of great concern, which requires infection control measures including antibacterial management and identification of β-lactamases-producing isolates.http://dx.doi.org/10.1155/2014/726179 |
| spellingShingle | Ali Hashemi Fatemeh Fallah Soroor Erfanimanesh Parastu Hamedani Shadi Alimehr Hossein Goudarzi Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran Scientifica |
| title | Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran |
| title_full | Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran |
| title_fullStr | Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran |
| title_full_unstemmed | Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran |
| title_short | Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran |
| title_sort | detection of β lactamases and outer membrane porins among klebsiella pneumoniae strains isolated in iran |
| url | http://dx.doi.org/10.1155/2014/726179 |
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