Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomized placebo-controlled trial.
<h4>Background</h4>Diabetes mellitus is associated with inadequate delivery of oxygen to tissues. Cellular hypoxia is associated with mitochondrial dysfunction which increases oxidative stress and hyperglycaemia. Hyperbaric oxygenation therapy, which was shown to improve insulin sensitiv...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254619&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832595725856276480 |
|---|---|
| author | Joan Khoo Christoph E Hagemeyer Darren C Henstridge Sumukh Kumble Ting-Yi Wang Rong Xu Linsey Gani Thomas King Shui-Boon Soh Troy Puar Vanessa Au Eberta Tan Tunn-Lin Tay Carmen Kam Eng-Kiong Teo |
| author_facet | Joan Khoo Christoph E Hagemeyer Darren C Henstridge Sumukh Kumble Ting-Yi Wang Rong Xu Linsey Gani Thomas King Shui-Boon Soh Troy Puar Vanessa Au Eberta Tan Tunn-Lin Tay Carmen Kam Eng-Kiong Teo |
| author_sort | Joan Khoo |
| collection | DOAJ |
| description | <h4>Background</h4>Diabetes mellitus is associated with inadequate delivery of oxygen to tissues. Cellular hypoxia is associated with mitochondrial dysfunction which increases oxidative stress and hyperglycaemia. Hyperbaric oxygenation therapy, which was shown to improve insulin sensitivity, is impractical for regular use. We evaluated the effects of water which is stably-enriched with oxygen (ELO water) to increase arterial blood oxygen levels, on mitochondrial function in the presence of normal- or high-glucose environments, and as glucose-lowering therapy in humans.<h4>Methods</h4>We compared arterial blood oxygen levels in Sprague-Dawley rats after 7 days of ad libitum ELO or tap water consumption. Mitochondrial stress testing, and flow cytometry analysis of mitochondrial mass and membrane potential, were performed on human HepG2 cells cultured in four Dulbecco's Modified Eagle Medium media, made with ELO water or regular (control) water, at normal (5.5 mM) or high (25 mM) glucose concentrations. We also randomized 150 adults with type 2 diabetes (mean age 53 years, glycated haemoglobin HbA1c 8.9% [74 mmol/mol], average duration of diabetes 12 years) to drink 1.5 litres daily of bottled ELO water or drinking water.<h4>Results</h4>ELO water raised arterial oxygen tension pO2 significantly (335 ± 26 vs. 188 ± 18 mmHg, p = 0.006) compared with tap water. In cells cultured in control water, mitochondrial mass and membrane potential were both significantly lower at 25 mM glucose compared with 5.5 mM glucose; in contrast, mitochondrial mass and membrane potential did not differ significantly at normal or high glucose concentrations in cells cultured in ELO water. The high-glucose environment induced a greater mitochondrial proton leak in cells cultured in ELO water compared to cells cultured in control medium at similar glucose concentration. In type 2 diabetic adults, HbA1c decreased significantly (p = 0.002) by 0.3 ± 0.7% (4 ± 8 mmol/mol), with ELO water after 12 weeks of treatment but was unchanged with placebo.<h4>Conclusions</h4>ELO water raises arterial blood oxygen levels, appears to have a protective effect on hyperglycaemia-induced reduction in mitochondrial mass and mitochondrial dysfunction, and may be effective adjuvant therapy for type 2 diabetes. |
| format | Article |
| id | doaj-art-fd26675b51944102bed758e92c5e99ba |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-fd26675b51944102bed758e92c5e99ba2025-01-18T05:31:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01167e025461910.1371/journal.pone.0254619Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomized placebo-controlled trial.Joan KhooChristoph E HagemeyerDarren C HenstridgeSumukh KumbleTing-Yi WangRong XuLinsey GaniThomas KingShui-Boon SohTroy PuarVanessa AuEberta TanTunn-Lin TayCarmen KamEng-Kiong Teo<h4>Background</h4>Diabetes mellitus is associated with inadequate delivery of oxygen to tissues. Cellular hypoxia is associated with mitochondrial dysfunction which increases oxidative stress and hyperglycaemia. Hyperbaric oxygenation therapy, which was shown to improve insulin sensitivity, is impractical for regular use. We evaluated the effects of water which is stably-enriched with oxygen (ELO water) to increase arterial blood oxygen levels, on mitochondrial function in the presence of normal- or high-glucose environments, and as glucose-lowering therapy in humans.<h4>Methods</h4>We compared arterial blood oxygen levels in Sprague-Dawley rats after 7 days of ad libitum ELO or tap water consumption. Mitochondrial stress testing, and flow cytometry analysis of mitochondrial mass and membrane potential, were performed on human HepG2 cells cultured in four Dulbecco's Modified Eagle Medium media, made with ELO water or regular (control) water, at normal (5.5 mM) or high (25 mM) glucose concentrations. We also randomized 150 adults with type 2 diabetes (mean age 53 years, glycated haemoglobin HbA1c 8.9% [74 mmol/mol], average duration of diabetes 12 years) to drink 1.5 litres daily of bottled ELO water or drinking water.<h4>Results</h4>ELO water raised arterial oxygen tension pO2 significantly (335 ± 26 vs. 188 ± 18 mmHg, p = 0.006) compared with tap water. In cells cultured in control water, mitochondrial mass and membrane potential were both significantly lower at 25 mM glucose compared with 5.5 mM glucose; in contrast, mitochondrial mass and membrane potential did not differ significantly at normal or high glucose concentrations in cells cultured in ELO water. The high-glucose environment induced a greater mitochondrial proton leak in cells cultured in ELO water compared to cells cultured in control medium at similar glucose concentration. In type 2 diabetic adults, HbA1c decreased significantly (p = 0.002) by 0.3 ± 0.7% (4 ± 8 mmol/mol), with ELO water after 12 weeks of treatment but was unchanged with placebo.<h4>Conclusions</h4>ELO water raises arterial blood oxygen levels, appears to have a protective effect on hyperglycaemia-induced reduction in mitochondrial mass and mitochondrial dysfunction, and may be effective adjuvant therapy for type 2 diabetes.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254619&type=printable |
| spellingShingle | Joan Khoo Christoph E Hagemeyer Darren C Henstridge Sumukh Kumble Ting-Yi Wang Rong Xu Linsey Gani Thomas King Shui-Boon Soh Troy Puar Vanessa Au Eberta Tan Tunn-Lin Tay Carmen Kam Eng-Kiong Teo Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomized placebo-controlled trial. PLoS ONE |
| title | Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomized placebo-controlled trial. |
| title_full | Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomized placebo-controlled trial. |
| title_fullStr | Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomized placebo-controlled trial. |
| title_full_unstemmed | Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomized placebo-controlled trial. |
| title_short | Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomized placebo-controlled trial. |
| title_sort | effects of water stably enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function and as adjuvant therapy for type 2 diabetes in a randomized placebo controlled trial |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254619&type=printable |
| work_keys_str_mv | AT joankhoo effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT christophehagemeyer effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT darrenchenstridge effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT sumukhkumble effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT tingyiwang effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT rongxu effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT linseygani effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT thomasking effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT shuiboonsoh effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT troypuar effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT vanessaau effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT ebertatan effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT tunnlintay effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT carmenkam effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial AT engkiongteo effectsofwaterstablyenrichedwithoxygenasanovelmethodoftissueoxygenationonmitochondrialfunctionandasadjuvanttherapyfortype2diabetesinarandomizedplacebocontrolledtrial |