Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy
Background: Cerebral palsy (CP) is a prevalent cause of physical disability in children, often resulting from hypoxic-ischemic encephalopathy, with current therapies often failing to address the underlying pathophysiology. This study aimed to investigate the potential synergistic effects of human am...
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Elsevier
2025-06-01
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| Series: | IBRO Neuroscience Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667242124001234 |
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| author | Yu Zhou Xu-Huan Li Lu-Na He Li-Na Wang Jing Zang Dong-Ming Wang Jing Gao Xue-feng Yu |
| author_facet | Yu Zhou Xu-Huan Li Lu-Na He Li-Na Wang Jing Zang Dong-Ming Wang Jing Gao Xue-feng Yu |
| author_sort | Yu Zhou |
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| description | Background: Cerebral palsy (CP) is a prevalent cause of physical disability in children, often resulting from hypoxic-ischemic encephalopathy, with current therapies often failing to address the underlying pathophysiology. This study aimed to investigate the potential synergistic effects of human amnion-derived mesenchymal stem cells (hAMSCs) combined with scalp acupuncture in a rat model of CP. Methods: Twenty male Sprague-Dawley rats were randomly divided into four groups: Sham, CP, hAMSCs, and hAMSCs+scalp acupuncture (hAMSCs+AP). The CP model was induced via left common carotid artery ligation. hAMSCs were administered through tail vein injection, followed by scalp acupuncture at Baihui (GV20) and Qubin (GB7) points. Neurobehavioral function was assessed using the Bederson score, and brain tissues were analyzed using hematoxylin and eosin (H&E) staining, TUNEL staining, and RT-qPCR for apoptosis-related genes. Results: The CP group exhibited significant neurobehavioral deficits and increased apoptosis. Both hAMSCs and hAMSCs+AP treatments improved neurobehavioral function and reduced apoptosis. The combination therapy further decreased apoptosis levels, normalized mRNA expression of Bax, Caspase 9, and Caspase 3, and alleviated histological damage. Conclusions: The combination of hAMSCs and scalp acupuncture provides a promising treatment for CP, potentially alleviating brain damage through apoptosis regulation. Further studies are required to elucidate the detailed mechanisms and assess clinical feasibility and safety. |
| format | Article |
| id | doaj-art-fcbe1165b3cf44da9480857f680fde6b |
| institution | Kabale University |
| issn | 2667-2421 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | IBRO Neuroscience Reports |
| spelling | doaj-art-fcbe1165b3cf44da9480857f680fde6b2025-01-29T05:02:22ZengElsevierIBRO Neuroscience Reports2667-24212025-06-0118263269Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsyYu Zhou0Xu-Huan Li1Lu-Na He2Li-Na Wang3Jing Zang4Dong-Ming Wang5Jing Gao6Xue-feng Yu7Department of Pediatric Rehabilitation, Huai’an Maternal and Child Health Care Hospital Affiliated to Yangzhou University Huai’an, Jiangsu 223021, ChinaDepartment of Orthopedics, Affiliated Rehabilitation Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330003, ChinaDepartment of Pediatric Rehabilitation, Huai’an Maternal and Child Health Care Hospital Affiliated to Yangzhou University Huai’an, Jiangsu 223021, ChinaDepartment of Pediatric Rehabilitation, Huai’an Maternal and Child Health Care Hospital Affiliated to Yangzhou University Huai’an, Jiangsu 223021, ChinaDepartment of Pediatric Rehabilitation, Huai’an Maternal and Child Health Care Hospital Affiliated to Yangzhou University Huai’an, Jiangsu 223021, ChinaDepartment of Pediatric Rehabilitation, Huai’an Maternal and Child Health Care Hospital Affiliated to Yangzhou University Huai’an, Jiangsu 223021, ChinaDepartment of Pediatric Rehabilitation, Huai’an Maternal and Child Health Care Hospital Affiliated to Yangzhou University Huai’an, Jiangsu 223021, China; Correspondence to: Department of Child Rehabilitation Division, Huai'an Maternal and Child Health Care Center Huai’an, Jiangsu 223021, China.Department of Orthopedics, Affiliated Rehabilitation Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330003, China; Department of Orthopedics, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330003, China; Corresponding author at: Department of Pediatric Rehabilitation, Huai’an Maternal and Child Health Care Hospital Affiliated to Yangzhou University Huai’an, Jiangsu 223021, China.Background: Cerebral palsy (CP) is a prevalent cause of physical disability in children, often resulting from hypoxic-ischemic encephalopathy, with current therapies often failing to address the underlying pathophysiology. This study aimed to investigate the potential synergistic effects of human amnion-derived mesenchymal stem cells (hAMSCs) combined with scalp acupuncture in a rat model of CP. Methods: Twenty male Sprague-Dawley rats were randomly divided into four groups: Sham, CP, hAMSCs, and hAMSCs+scalp acupuncture (hAMSCs+AP). The CP model was induced via left common carotid artery ligation. hAMSCs were administered through tail vein injection, followed by scalp acupuncture at Baihui (GV20) and Qubin (GB7) points. Neurobehavioral function was assessed using the Bederson score, and brain tissues were analyzed using hematoxylin and eosin (H&E) staining, TUNEL staining, and RT-qPCR for apoptosis-related genes. Results: The CP group exhibited significant neurobehavioral deficits and increased apoptosis. Both hAMSCs and hAMSCs+AP treatments improved neurobehavioral function and reduced apoptosis. The combination therapy further decreased apoptosis levels, normalized mRNA expression of Bax, Caspase 9, and Caspase 3, and alleviated histological damage. Conclusions: The combination of hAMSCs and scalp acupuncture provides a promising treatment for CP, potentially alleviating brain damage through apoptosis regulation. Further studies are required to elucidate the detailed mechanisms and assess clinical feasibility and safety.http://www.sciencedirect.com/science/article/pii/S2667242124001234Cerebral palsyHypoxic-ischemic encephalopathyMesenchymal stem cellsScalp acupunctureApoptosis |
| spellingShingle | Yu Zhou Xu-Huan Li Lu-Na He Li-Na Wang Jing Zang Dong-Ming Wang Jing Gao Xue-feng Yu Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy IBRO Neuroscience Reports Cerebral palsy Hypoxic-ischemic encephalopathy Mesenchymal stem cells Scalp acupuncture Apoptosis |
| title | Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy |
| title_full | Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy |
| title_fullStr | Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy |
| title_full_unstemmed | Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy |
| title_short | Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy |
| title_sort | combined therapy of human amnion derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy |
| topic | Cerebral palsy Hypoxic-ischemic encephalopathy Mesenchymal stem cells Scalp acupuncture Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2667242124001234 |
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